Abstract
A class C beta-lactamase has been purified from the culture supernatant of the antarctic psychrophile Psychrobacter immobilis A8. This psychrophilic beta-lactamase displays a low level of thermal stability and a low optimal temperature of activity. In contrast to other cold-adapted enzymes, its level of specific activity is not higher than that of mesophilic class C beta-lactamases.
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Selected References
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- Ambler R. P. The structure of beta-lactamases. Philos Trans R Soc Lond B Biol Sci. 1980 May 16;289(1036):321–331. doi: 10.1098/rstb.1980.0049. [DOI] [PubMed] [Google Scholar]
- Cartwright S. J., Waley S. G. Purification of beta-lactamases by affinity chromatography on phenylboronic acid-agarose. Biochem J. 1984 Jul 15;221(2):505–512. doi: 10.1042/bj2210505. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davail S., Feller G., Narinx E., Gerday C. Cold adaptation of proteins. Purification, characterization, and sequence of the heat-labile subtilisin from the antarctic psychrophile Bacillus TA41. J Biol Chem. 1994 Jul 1;269(26):17448–17453. [PubMed] [Google Scholar]
- Feller G., Lonhienne T., Deroanne C., Libioulle C., Van Beeumen J., Gerday C. Purification, characterization, and nucleotide sequence of the thermolabile alpha-amylase from the antarctic psychrotroph Alteromonas haloplanctis A23. J Biol Chem. 1992 Mar 15;267(8):5217–5221. [PubMed] [Google Scholar]
- Galleni M., Amicosante G., Frère J. M. A survey of the kinetic parameters of class C beta-lactamases. Cephalosporins and other beta-lactam compounds. Biochem J. 1988 Oct 1;255(1):123–129. doi: 10.1042/bj2550123. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Galleni M., Frère J. M. A survey of the kinetic parameters of class C beta-lactamases. Penicillins. Biochem J. 1988 Oct 1;255(1):119–122. doi: 10.1042/bj2550119. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ghuysen J. M. Serine beta-lactamases and penicillin-binding proteins. Annu Rev Microbiol. 1991;45:37–67. doi: 10.1146/annurev.mi.45.100191.000345. [DOI] [PubMed] [Google Scholar]
- Gounot A. M. Bacterial life at low temperature: physiological aspects and biotechnological implications. J Appl Bacteriol. 1991 Nov;71(5):386–397. doi: 10.1111/j.1365-2672.1991.tb03806.x. [DOI] [PubMed] [Google Scholar]
- Inniss W. E. Interaction of temperature and psychrophilic microorganisms. Annu Rev Microbiol. 1975;29:445–465. doi: 10.1146/annurev.mi.29.100175.002305. [DOI] [PubMed] [Google Scholar]
- Lamotte-Brasseur J., Knox J., Kelly J. A., Charlier P., Fonzé E., Dideberg O., Frére J. M. The structures and catalytic mechanisms of active-site serine beta-lactamases. Biotechnol Genet Eng Rev. 1994;12:189–230. doi: 10.1080/02648725.1994.10647912. [DOI] [PubMed] [Google Scholar]
- Lodge J. M., Minchin S. D., Piddock L. J., Busby S. J. Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase. Biochem J. 1990 Dec 15;272(3):627–631. doi: 10.1042/bj2720627. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morita R. Y. Psychrophilic bacteria. Bacteriol Rev. 1975 Jun;39(2):144–167. doi: 10.1128/br.39.2.144-167.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]