Abstract
PCR was used to detect and specifically identify a gene fragment from Cryptosporidium parvum. An 873-bp region of a 2,359-bp DNA fragment encoding a repetitive oocyst protein of C. parvum was shown to be specifically amplified in C. parvum. An excystation protocol before DNA extraction allowed the differentiation between live and dead Cryptosporidium parvum oocysts.
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Selected References
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- Arrowood M. J., Sterling C. R. Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. J Parasitol. 1987 Apr;73(2):314–319. [PubMed] [Google Scholar]
- Fayer R., Leek R. G. The effects of reducing conditions, medium, pH, temperature, and time on in vitro excystation of Cryptosporidium. J Protozool. 1984 Nov;31(4):567–569. doi: 10.1111/j.1550-7408.1984.tb05504.x. [DOI] [PubMed] [Google Scholar]
- Hayes E. B., Matte T. D., O'Brien T. R., McKinley T. W., Logsdon G. S., Rose J. B., Ungar B. L., Word D. M., Pinsky P. F., Cummings M. L. Large community outbreak of cryptosporidiosis due to contamination of a filtered public water supply. N Engl J Med. 1989 May 25;320(21):1372–1376. doi: 10.1056/NEJM198905253202103. [DOI] [PubMed] [Google Scholar]
- Lally N. C., Baird G. D., McQuay S. J., Wright F., Oliver J. J. A 2359-base pair DNA fragment from Cryptosporidium parvum encoding a repetitive oocyst protein. Mol Biochem Parasitol. 1992 Nov;56(1):69–78. doi: 10.1016/0166-6851(92)90155-d. [DOI] [PubMed] [Google Scholar]
- Olson B. H. The safety of our drinking water: reason for concern but not alarm. N Engl J Med. 1989 May 25;320(21):1413–1414. doi: 10.1056/NEJM198905253202111. [DOI] [PubMed] [Google Scholar]
- Ongerth J. E., Stibbs H. H. Identification of Cryptosporidium oocysts in river water. Appl Environ Microbiol. 1987 Apr;53(4):672–676. doi: 10.1128/aem.53.4.672-676.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rush B. A., Chapman P. A., Ineson R. W. A probable waterborne outbreak of cryptosporidiosis in the Sheffield area. J Med Microbiol. 1990 Aug;32(4):239–242. doi: 10.1099/00222615-32-4-239. [DOI] [PubMed] [Google Scholar]
- Smith H. V., Patterson W. J., Hardie R., Greene L. A., Benton C., Tulloch W., Gilmour R. A., Girdwood R. W., Sharp J. C., Forbes G. I. An outbreak of waterborne cryptosporidiosis caused by post-treatment contamination. Epidemiol Infect. 1989 Dec;103(3):703–715. doi: 10.1017/s0950268800031101. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wagner C., Kimmig P. Cryptosporidium parvum und Giardia lamblia--Vorkommen in Oberflächen- und Trinkwasser--Bedeutung und Nachweisverfahren. Gesundheitswesen. 1992 Nov;54(11):662–665. [PubMed] [Google Scholar]
- Waldman E., Tzipori S., Forsyth J. R. Separation of Cryptosporidium species oocysts from feces by using a percoll discontinuous density gradient. J Clin Microbiol. 1986 Jan;23(1):199–200. doi: 10.1128/jcm.23.1.199-200.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]