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. 1996 Jan;62(1):275–278. doi: 10.1128/aem.62.1.275-278.1996

Detection of specific bacterial cells with 2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate and fast red TR in situ hybridization.

N Yamaguchi 1, S Inaoka 1, K Tani 1, T Kenzaka 1, M Nasu 1
PMCID: PMC167796  PMID: 8572706

Abstract

An in situ hybridization technique with HNPP (2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate) and Fast Red TR was used to detect specific bacterial cells at the single-cell level. By this technique, the fluorescent signals of target bacterial cells were up to eight times more intense than those of standard fluorescence in situ hybridization with mono-fluorescein isothiocyanate-labeled oligonucleotide probes. This novel HNPP-Fast Red TR whole-cell hybridization technique is available for the identification of small or low-rRNA-content bacterial cells in the natural environment.

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Selected References

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