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. 1996 Mar;62(3):1084–1088. doi: 10.1128/aem.62.3.1084-1088.1996

Utilization of individual cellodextrins by three predominant ruminal cellulolytic bacteria.

Y Shi 1, P J Weimer 1
PMCID: PMC167871  PMID: 8975600

Abstract

Growth of the ruminal bacteria Fibrobacter succinogenes S85, Ruminococcus flavefaciens FD-1, and R. albus 7 followed Monod kinetics with respect to concentrations of individual pure cellodextrins (cellobiose, cellotriose, cellotetraose, cellopentaose, and cellohexaose). Under the conditions tested, R. flavefaciens FD-1 possesses the greatest capacity to compete for low concentrations of these cellodextrins.

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Selected References

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  1. Balch W. E., Wolfe R. S. New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere. Appl Environ Microbiol. 1976 Dec;32(6):781–791. doi: 10.1128/aem.32.6.781-791.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bryant M. P. Nutritional requirements of the predominant rumen cellulolytic bacteria. Fed Proc. 1973 Jul;32(7):1809–1813. [PubMed] [Google Scholar]
  3. Chow J. M., Russell J. B. Effect of pH and Monensin on Glucose Transport by Fibrobacter succinogenes, a Cellulolytic Ruminal Bacterium. Appl Environ Microbiol. 1992 Apr;58(4):1115–1120. doi: 10.1128/aem.58.4.1115-1120.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Helaszek C. T., White B. A. Cellobiose uptake and metabolism by Ruminococcus flavefaciens. Appl Environ Microbiol. 1991 Jan;57(1):64–68. doi: 10.1128/aem.57.1.64-68.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hengge R., Boos W. Maltose and lactose transport in Escherichia coli. Examples of two different types of concentrative transport systems. Biochim Biophys Acta. 1983 Aug 11;737(3-4):443–478. doi: 10.1016/0304-4157(83)90009-6. [DOI] [PubMed] [Google Scholar]
  6. Hiltner P., Dehority B. A. Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria. Appl Environ Microbiol. 1983 Sep;46(3):642–648. doi: 10.1128/aem.46.3.642-648.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Huang L., Forsberg C. W., Thomas D. Y. Purification and characterization of a chloride-stimulated cellobiosidase from Bacteroides succinogenes S85. J Bacteriol. 1988 Jul;170(7):2923–2932. doi: 10.1128/jb.170.7.2923-2932.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Latham M. J., Brooker B. E., Pettipher G. L., Harris P. J. Adhesion of Bacteroides succinogenes in pure culture and in the presence of Ruminococcus flavefaciens to cell walls in leaves of perennial ryegrass (Lolium perenne). Appl Environ Microbiol. 1978 Jun;35(6):1166–1173. doi: 10.1128/aem.35.6.1166-1173.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Maas L. K., Glass T. L. Cellobiose uptake by the cellulolytic ruminal anaerobe Fibrobacter (Bacteroides) succinogenes. Can J Microbiol. 1991 Feb;37(2):141–147. doi: 10.1139/m91-021. [DOI] [PubMed] [Google Scholar]
  10. Odenyo A. A., Mackie R. I., Stahl D. A., White B. A. The use of 16S rRNA-targeted oligonucleotide probes to study competition between ruminal fibrolytic bacteria: development of probes for Ruminococcus species and evidence for bacteriocin production. Appl Environ Microbiol. 1994 Oct;60(10):3688–3696. doi: 10.1128/aem.60.10.3688-3696.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rasmussen M. A., Hespell R. B., White B. A., Bothast R. J. Inhibitory Effects of Methylcellulose on Cellulose Degradation by Ruminococcus flavefaciens. Appl Environ Microbiol. 1988 Apr;54(4):890–897. doi: 10.1128/aem.54.4.890-897.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Russell J. B., Dombrowski D. B. Effect of pH on the efficiency of growth by pure cultures of rumen bacteria in continuous culture. Appl Environ Microbiol. 1980 Mar;39(3):604–610. doi: 10.1128/aem.39.3.604-610.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Russell J. B. Fermentation of cellodextrins by cellulolytic and noncellulolytic rumen bacteria. Appl Environ Microbiol. 1985 Mar;49(3):572–576. doi: 10.1128/aem.49.3.572-576.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Thurston B., Dawson K. A., Strobel H. J. Cellobiose versus glucose utilization by the ruminal bacterium Ruminococcus albus. Appl Environ Microbiol. 1993 Aug;59(8):2631–2637. doi: 10.1128/aem.59.8.2631-2637.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wachenheim D. E., Hespell R. B. Responses of Ruminococcus flavefaciens, a Ruminal Cellulolytic Species, to Nutrient Starvation. Appl Environ Microbiol. 1985 Dec;50(6):1361–1367. doi: 10.1128/aem.50.6.1361-1367.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wells J. E., Russell J. B., Shi Y., Weimer P. J. Cellodextrin efflux by the cellulolytic ruminal bacterium Fibrobacter succinogenes and its potential role in the growth of nonadherent bacteria. Appl Environ Microbiol. 1995 May;61(5):1757–1762. doi: 10.1128/aem.61.5.1757-1762.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]

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