Abstract
Linocin M18 is an antilisterial bacteriocin produced by the red smear cheese bacterium Brevibacterium linens M18. Oligonucleotide probes based on the N-terminal amino acid sequence were used to locate its single copy gene, lin, on the chromosomal DNA. The amino acid composition, N-terminal sequence, and molecular mass derived from the nucleotide sequence of an open reading frame of 798 nucleotides coding for 266 amino acids found on a 3-kb BamHI restriction fragment correspond closely to those obtained from the purified protein (N. Valdés-Stauber and S. Scherer, Appl. Environ. Microbiol. 60:3809-3814, 1994). No sequence homology to any protein or nucleotide sequences deposited in databases was found. Comparison of the nucleotide sequence and the N-terminal amino acid sequence derived from the protein suggests that B. linens M18 produces an N-formyl-methionyl-CAC tRNA. A wide taxonomical distribution of the gene within coryneform bacteria has been demonstrated by PCR amplification. The structural gene from linocin M18 is present at least in three Brevibacterium species, five Arthrobacter species, and five Corynebacterium species.
Full Text
The Full Text of this article is available as a PDF (264.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barefoot S. F., Klaenhammer T. R. Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus. Appl Environ Microbiol. 1983 Jun;45(6):1808–1815. doi: 10.1128/aem.45.6.1808-1815.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Diep D. B., Håvarstein L. S., Nissen-Meyer J., Nes I. F. The gene encoding plantaricin A, a bacteriocin from Lactobacillus plantarum C11, is located on the same transcription unit as an agr-like regulatory system. Appl Environ Microbiol. 1994 Jan;60(1):160–166. doi: 10.1128/aem.60.1.160-166.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Flamm R. K., Hinrichs D. J., Thomashow M. F. Introduction of pAM beta 1 into Listeria monocytogenes by conjugation and homology between native L. monocytogenes plasmids. Infect Immun. 1984 Apr;44(1):157–161. doi: 10.1128/iai.44.1.157-161.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grinstead D. A., Barefoot S. F. Jenseniin G, a heat-stable bacteriocin produced by Propionibacterium jensenii P126. Appl Environ Microbiol. 1992 Jan;58(1):215–220. doi: 10.1128/aem.58.1.215-220.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holzapfel W. H., Geisen R., Schillinger U. Biological preservation of foods with reference to protective cultures, bacteriocins and food-grade enzymes. Int J Food Microbiol. 1995 Jan;24(3):343–362. doi: 10.1016/0168-1605(94)00036-6. [DOI] [PubMed] [Google Scholar]
- Jack R. W., Tagg J. R., Ray B. Bacteriocins of gram-positive bacteria. Microbiol Rev. 1995 Jun;59(2):171–200. doi: 10.1128/mr.59.2.171-200.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Joerger M. C., Klaenhammer T. R. Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481. J Bacteriol. 1986 Aug;167(2):439–446. doi: 10.1128/jb.167.2.439-446.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klaenhammer T. R. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol Rev. 1993 Sep;12(1-3):39–85. doi: 10.1111/j.1574-6976.1993.tb00012.x. [DOI] [PubMed] [Google Scholar]
- Liebl W., Ehrmann M., Ludwig W., Schleifer K. H. Transfer of Brevibacterium divaricatum DSM 20297T, "Brevibacterium flavum" DSM 20411, "Brevibacterium lactofermentum" DSM 20412 and DSM 1412, and Corynebacterium glutamicum and their distinction by rRNA gene restriction patterns. Int J Syst Bacteriol. 1991 Apr;41(2):255–260. doi: 10.1099/00207713-41-2-255. [DOI] [PubMed] [Google Scholar]
- Maisnier-Patin S., Richard J. Activity and purification of linenscin OC2, an antibacterial substance produced by Brevibacterium linens OC2, an orange cheese coryneform bacterium. Appl Environ Microbiol. 1995 May;61(5):1847–1852. doi: 10.1128/aem.61.5.1847-1852.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ryser E. T., Maisnier-Patin S., Gratadoux J. J., Richard J. Isolation and identification of cheese-smear bacteria inhibitory to Listeria spp. Int J Food Microbiol. 1994 Feb;21(3):237–246. doi: 10.1016/0168-1605(94)90030-2. [DOI] [PubMed] [Google Scholar]
- Valdes-Stauber N., Götz H., Busse M. Antagonistic effect of coryneform bacteria from red smear cheese against Listeria species. Int J Food Microbiol. 1991 Jun;13(2):119–130. doi: 10.1016/0168-1605(91)90054-s. [DOI] [PubMed] [Google Scholar]
- Valdés-Stauber N., Scherer S. Isolation and characterization of Linocin M18, a bacteriocin produced by Brevibacterium linens. Appl Environ Microbiol. 1994 Oct;60(10):3809–3814. doi: 10.1128/aem.60.10.3809-3814.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]