Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1996 Apr;62(4):1424–1427. doi: 10.1128/aem.62.4.1424-1427.1996

Detection of infectious enteroviruses by an integrated cell culture-PCR procedure.

K A Reynolds 1, C P Gerba 1, I L Pepper 1
PMCID: PMC167909  PMID: 8919804

Abstract

Rapid detection of infectious enteroviruses in environmental samples was made possible by utilizing an integrated cell culture-reverse transcriptase PCR approach. By this method, the presence of infectious enterovirus was confirmed within 24 h, compared with > or = 3 days by cell culture alone. The combined methodology eliminated typical problems normally associated with direct reverse transcriptase PCR by increasing the equivalent volume of environmental sample examined and reducing the effects of inhibitory compounds.

Full Text

The Full Text of this article is available as a PDF (203.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abbaszadegan M., Huber M. S., Gerba C. P., Pepper I. L. Detection of enteroviruses in groundwater with the polymerase chain reaction. Appl Environ Microbiol. 1993 May;59(5):1318–1324. doi: 10.1128/aem.59.5.1318-1324.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ansari S. A., Farrah S. R., Chaudhry G. R. Presence of human immunodeficiency virus nucleic acids in wastewater and their detection by polymerase chain reaction. Appl Environ Microbiol. 1992 Dec;58(12):3984–3990. doi: 10.1128/aem.58.12.3984-3990.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bej A. K., Mahbubani M. H., Dicesare J. L., Atlas R. M. Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples. Appl Environ Microbiol. 1991 Dec;57(12):3529–3534. doi: 10.1128/aem.57.12.3529-3534.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Farrah S. R., Goyal S. M., Gerba C. P., Wallis C., Melnick J. L. Concentration of poliovirus from tap water onto membrane filters with aluminum chloride at ambient pH levels. Appl Environ Microbiol. 1978 Mar;35(3):624–626. doi: 10.1128/aem.35.3.624-626.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gerba C. P., Farrah S. R., Goyal S. M., Wallis C., Melnick J. L. Concentration of enteroviruses from large volumes of tap water, treated sewage, and seawater. Appl Environ Microbiol. 1978 Mar;35(3):540–548. doi: 10.1128/aem.35.3.540-548.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Katzenelson E., Fattal B., Hostovesky T. Organic flocculation: an efficient second-step concentration method for the detection of viruses in tap water. Appl Environ Microbiol. 1976 Oct;32(4):638–639. doi: 10.1128/aem.32.4.638-639.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kopecka H., Dubrou S., Prevot J., Marechal J., López-Pila J. M. Detection of naturally occurring enteroviruses in waters by reverse transcription, polymerase chain reaction, and hybridization. Appl Environ Microbiol. 1993 Apr;59(4):1213–1219. doi: 10.1128/aem.59.4.1213-1219.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ma J. F., Gerba C. P., Pepper I. L. Increased sensitivity of poliovirus detection in tap water concentrates by reverse transcriptase-polymerase chain reaction. J Virol Methods. 1995 Nov;55(3):295–302. doi: 10.1016/0166-0934(95)00065-6. [DOI] [PubMed] [Google Scholar]
  9. Pillai S. D., Josephson K. L., Bailey R. L., Gerba C. P., Pepper I. L. Rapid method for processing soil samples for polymerase chain reaction amplification of specific gene sequences. Appl Environ Microbiol. 1991 Aug;57(8):2283–2286. doi: 10.1128/aem.57.8.2283-2286.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schwab K. J., De Leon R., Sobsey M. D. Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR. Appl Environ Microbiol. 1995 Feb;61(2):531–537. doi: 10.1128/aem.61.2.531-537.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Straub T. M., Pepper I. L., Abbaszadegan M., Gerba C. P. A method to detect enteroviruses in sewage sludge-amended soil using the PCR. Appl Environ Microbiol. 1994 Mar;60(3):1014–1017. doi: 10.1128/aem.60.3.1014-1017.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tsai Y. L., Sobsey M. D., Sangermano L. R., Palmer C. J. Simple method of concentrating enteroviruses and hepatitis A virus from sewage and ocean water for rapid detection by reverse transcriptase-polymerase chain reaction. Appl Environ Microbiol. 1993 Oct;59(10):3488–3491. doi: 10.1128/aem.59.10.3488-3491.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wallis C., Melnick J. L., Gerba C. P. Concentration of viruses from water by membrane chromatography. Annu Rev Microbiol. 1979;33:413–437. doi: 10.1146/annurev.mi.33.100179.002213. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES