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. 1996 Apr;62(4):1441–1443. doi: 10.1128/aem.62.4.1441-1443.1996

Sporulation-promoting ability of Clostridium perfringens culture fluids.

N J Shih 1, R G Labbé 1
PMCID: PMC167913  PMID: 8919808

Abstract

The culture supernatant fluids (CSFs) of 12 strains of Clostridium perfringens types A, B, C, and D stimulated sporulation of test strains NCTC 8238 and NCTC 8449 of this organism. The sporulation-promoting ability was present in vegetative and sporulating CSFs of both enterotoxin-positive (Ent+) and Ent- strains. The sporulation factor possessed a molecular weight between 1,000 and 5,000 and was heat and acid stable. This study suggests a potential role for Ent- strains in food-borne disease outbreaks caused by Ent+ strains of C. perfringens type A.

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Selected References

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  1. Dillon M. E., Labbe R. G. Stimulation of growth and sporulation of Clostridium perfringens by its homologous enterotoxin. FEMS Microbiol Lett. 1989 May;50(1-2):221–224. doi: 10.1016/0378-1097(89)90490-4. [DOI] [PubMed] [Google Scholar]
  2. Duncan C. L., Strong D. H., Sebald M. Sporulation and enterotoxin production by mutants of Clostridium perfringens. J Bacteriol. 1972 Apr;110(1):378–391. doi: 10.1128/jb.110.1.378-391.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Errington J. Bacillus subtilis sporulation: regulation of gene expression and control of morphogenesis. Microbiol Rev. 1993 Mar;57(1):1–33. doi: 10.1128/mr.57.1.1-33.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Frieben W. R., Duncan C. L. Homology between enterotoxin protein and spore structural protein in Clostridium perfringens type A. Eur J Biochem. 1973 Nov 15;39(2):393–401. doi: 10.1111/j.1432-1033.1973.tb03137.x. [DOI] [PubMed] [Google Scholar]
  5. Grossman A. D., Losick R. Extracellular control of spore formation in Bacillus subtilis. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4369–4373. doi: 10.1073/pnas.85.12.4369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heredia N. L., Labbe R. G., Rodriguez M. A., Garcia-Alvarado J. S. Growth, sporulation and enterotoxin production by Clostridium perfringens type A in the presence of human bile salts. FEMS Microbiol Lett. 1991 Nov 1;68(1):15–21. doi: 10.1016/0378-1097(91)90387-p. [DOI] [PubMed] [Google Scholar]
  7. Labbe R. G., Nolan L. L. Stimulation of Clostridium perfringens enterotoxin formation by caffeine and theobromine. Infect Immun. 1981 Oct;34(1):50–54. doi: 10.1128/iai.34.1.50-54.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McDonel J. L. Clostridium perfringens toxins (type A, B, C, D, E). Pharmacol Ther. 1980;10(3):617–655. doi: 10.1016/0163-7258(80)90031-5. [DOI] [PubMed] [Google Scholar]
  9. Melville S. B., Labbe R., Sonenshein A. L. Expression from the Clostridium perfringens cpe promoter in C. perfringens and Bacillus subtilis. Infect Immun. 1994 Dec;62(12):5550–5558. doi: 10.1128/iai.62.12.5550-5558.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Naik H. S., Duncan C. L. Rapid detection and quantitation of Clostridium perfringens enterostoxin by counterimmunoelectrophoresis. Appl Environ Microbiol. 1977 Aug;34(2):125–128. doi: 10.1128/aem.34.2.125-128.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sacks L. E., Thompson P. A. Increased spore yields of Clostridium perfringens in the presence of methylxanthines. Appl Environ Microbiol. 1977 Aug;34(2):189–193. doi: 10.1128/aem.34.2.189-193.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Srinivasan V. R. Sporogen--an "inductor" for bacterial cell division. Nature. 1966 Jan 29;209(5022):537–537. doi: 10.1038/209537a0. [DOI] [PubMed] [Google Scholar]
  13. Waldburger C., Gonzalez D., Chambliss G. H. Characterization of a new sporulation factor in Bacillus subtilis. J Bacteriol. 1993 Oct;175(19):6321–6327. doi: 10.1128/jb.175.19.6321-6327.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wrigley D. M., Hanwella H. D., Thon B. L. Acid exposure enhances sporulation of certain strains of Clostridium perfringens. Anaerobe. 1995 Oct;1(5):263–267. doi: 10.1006/anae.1995.1025. [DOI] [PubMed] [Google Scholar]

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