Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1996 Apr;62(4):1481–1486. doi: 10.1128/aem.62.4.1481-1486.1996

Characterization and sequence analysis of a stable cryptic plasmid from Enterococcus faecium 226 and development of a stable cloning vector.

H A Wyckoff 1, M Barnes 1, K O Gillies 1, W E Sandine 1
PMCID: PMC167923  PMID: 8919818

Abstract

A small cryptic plasmid, pMBB1, isolated from Enterococcus faecium 226 was characterized. The plasmid contained an extremely stable replicon which has limited homology to the lactococcal plasmid pCI305. Sequence analysis of the replicon detected one open reading frame of 822 bp capable of encoding a 32-kDa protein. No detectable single-stranded intermediates were found for the replicon, suggesting that pMBB1 may be included in the same family as pCI305, although pCI305 exhibits a more narrow host range. A small stably maintained vector able to replicate in a variety of lactic acid bacteria, containing a large multiple cloning region, was constructed by using the pMBB1 replicon.

Full Text

The Full Text of this article is available as a PDF (282.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. G., McKay L. L. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol. 1983 Sep;46(3):549–552. doi: 10.1128/aem.46.3.549-552.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ballester S., Lopez P., Espinosa M., Alonso J. C., Lacks S. A. Plasmid structural instability associated with pC194 replication functions. J Bacteriol. 1989 May;171(5):2271–2277. doi: 10.1128/jb.171.5.2271-2277.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Casadaban M. J., Cohen S. N. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980 Apr;138(2):179–207. doi: 10.1016/0022-2836(80)90283-1. [DOI] [PubMed] [Google Scholar]
  4. Clewell D. B., Yagi Y., Dunny G. M., Schultz S. K. Characterization of three plasmid deoxyribonucleic acid molecules in a strain of Streptococcus faecalis: identification of a plasmid determining erythromycin resistance. J Bacteriol. 1974 Jan;117(1):283–289. doi: 10.1128/jb.117.1.283-289.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dornan S., Collins M. A. High efficiency electroporation of Lactococcus lactis subsp. lactis LM0230 with plasmid pGB301. Lett Appl Microbiol. 1990 Aug;11(2):62–64. doi: 10.1111/j.1472-765x.1990.tb01275.x. [DOI] [PubMed] [Google Scholar]
  6. Efstathiou J. D., McKay L. L. Inorganic salts resistance associated with a lactose-fermenting plasmid in Streptococcus lactis. J Bacteriol. 1977 Apr;130(1):257–265. doi: 10.1128/jb.130.1.257-265.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fahnestock S. R., Alexander P., Nagle J., Filpula D. Gene for an immunoglobulin-binding protein from a group G streptococcus. J Bacteriol. 1986 Sep;167(3):870–880. doi: 10.1128/jb.167.3.870-880.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hardy K. J., Peterlin B. M., Atchison R. E., Stobo J. D. Regulation of expression of the human interferon gamma gene. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8173–8177. doi: 10.1073/pnas.82.23.8173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hawley D. K., McClure W. R. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983 Apr 25;11(8):2237–2255. doi: 10.1093/nar/11.8.2237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hayes F., Daly C., Fitzgerald G. F. Identification of the Minimal Replicon of Lactococcus lactis subsp. lactis UC317 Plasmid pCI305. Appl Environ Microbiol. 1990 Jan;56(1):202–209. doi: 10.1128/aem.56.1.202-209.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hayes F., Vos P., Fitzgerald G. F., de Vos W. M., Daly C. Molecular organization of the minimal replicon of novel, narrow-host-range, lactococcal plasmid pCI305. Plasmid. 1991 Jan;25(1):16–26. doi: 10.1016/0147-619x(91)90003-f. [DOI] [PubMed] [Google Scholar]
  12. Jannière L., Bruand C., Ehrlich S. D. Structurally stable Bacillus subtilis cloning vectors. Gene. 1990 Mar 1;87(1):53–61. doi: 10.1016/0378-1119(90)90495-d. [DOI] [PubMed] [Google Scholar]
  13. Kiewiet R., Kok J., Seegers J. F., Venema G., Bron S. The Mode of Replication Is a Major Factor in Segregational Plasmid Instability in Lactococcus lactis. Appl Environ Microbiol. 1993 Feb;59(2):358–364. doi: 10.1128/aem.59.2.358-364.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kok J., van der Vossen J. M., Venema G. Construction of plasmid cloning vectors for lactic streptococci which also replicate in Bacillus subtilis and Escherichia coli. Appl Environ Microbiol. 1984 Oct;48(4):726–731. doi: 10.1128/aem.48.4.726-731.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Leenhouts K. J., Tolner B., Bron S., Kok J., Venema G., Seegers J. F. Nucleotide sequence and characterization of the broad-host-range lactococcal plasmid pWVO1. Plasmid. 1991 Jul;26(1):55–66. doi: 10.1016/0147-619x(91)90036-v. [DOI] [PubMed] [Google Scholar]
  16. Michel B., Ehrlich S. D. Illegitimate recombination occurs between the replication origin of the plasmid pC194 and a progressing replication fork. EMBO J. 1986 Dec 20;5(13):3691–3696. doi: 10.1002/j.1460-2075.1986.tb04701.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Niaudet B., Jannière L., Ehrlich S. D. Recombination between repeated DNA sequences occurs more often in plasmids than in the chromosome of Bacillus subtilis. Mol Gen Genet. 1984;197(1):46–54. doi: 10.1007/BF00327921. [DOI] [PubMed] [Google Scholar]
  18. Scott J. R. Regulation of plasmid replication. Microbiol Rev. 1984 Mar;48(1):1–23. doi: 10.1016/b978-0-12-048850-6.50006-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Seegers J. F., Bron S., Franke C. M., Venema G., Kiewiet R. The majority of lactococcal plasmids carry a highly related replicon. Microbiology. 1994 Jun;140(Pt 6):1291–1300. doi: 10.1099/00221287-140-6-1291. [DOI] [PubMed] [Google Scholar]
  20. Terzaghi B. E., Sandine W. E. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. doi: 10.1128/am.29.6.807-813.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vieira J., Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 1987;153:3–11. doi: 10.1016/0076-6879(87)53044-0. [DOI] [PubMed] [Google Scholar]
  22. Xu F. F., Pearce L. E., Yu P. L. Genetic analysis of a lactococcal plasmid replicon. Mol Gen Genet. 1991 May;227(1):33–39. doi: 10.1007/BF00260703. [DOI] [PubMed] [Google Scholar]
  23. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
  24. van der Vossen J. M., van der Lelie D., Venema G. Isolation and characterization of Streptococcus cremoris Wg2-specific promoters. Appl Environ Microbiol. 1987 Oct;53(10):2452–2457. doi: 10.1128/aem.53.10.2452-2457.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES