Abstract
Bacteriocins, including nisin, pediocin PO2, brevicin 286, and piscicolin 126, were extracted from fermentation media by adsorption onto Micro-Cel (a food-grade diatomite calcium silicate anticaking agent) and subsequent desorption. The optimal conditions for desorption of piscicolin 126 were determined and applied to other bacteriocins, and the relative purities of the desorbed preparations were compared. Piscicolin was not successfully desorbed from Micro-Cel at pH 1.0 to 12.0, with organic solvents, or by increase of ionic strength up to 1 M NaCl. However, 25 and 75% of the bacteriocin activity was desorbed by using 1% sodium deoxycholate and 1% sodium dodecyl sulfate (SDS), respectively. Higher levels (up to 100%) of desorption were achieved by repeated elution or by an increase in surfactant concentration. Desorption of piscicolin with 1/10 volume of SDS solution resulted in a preparation with 10 times concentration in activity, equivalent to that of ammonium sulfate preparations (409,600 to 819,200 activity units/ml). Determination of organic nitrogen (N) content revealed that the desorbed piscicolin preparations were substantially free of proteinaceous substances (approximately 92 to 99%) compared with original culture supernatants and ammonium sulfate preparations. Nisin, pediocin, and brevicin were also desorbed with 1% SDS with a similar level of purification.
Full Text
The Full Text of this article is available as a PDF (204.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baranova I. P., Egorov N. S., Grushina V. A. Izuchenie sorbtsii i desorbtsii nizina na razlichnykh kremnezemnykh adsorbentakh. Antibiot Med Biotekhnol. 1987 Jun;32(6):437–439. [PubMed] [Google Scholar]
- Baranova I. P., Grushina V. A., Egorov N. S. Ispol'zovanie adsorbentov dlia optimizatsii protsessa vydeleniia nizina iz kul'tural'noi zhidkosti. Antibiotiki. 1984 Sep;29(9):643–645. [PubMed] [Google Scholar]
- Baranova I. P., Grushina V. A., Nikitin Iu S., Egorov N. S., Polin A. N. Adsorbtsiia nizina na kremnezemnykh adsorbentakh. Antibiotiki. 1983 Apr;28(4):258–262. [PubMed] [Google Scholar]
- Barefoot S. F., Klaenhammer T. R. Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus. Appl Environ Microbiol. 1983 Jun;45(6):1808–1815. doi: 10.1128/aem.45.6.1808-1815.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bower C. K., McGuire J., Daeschel M. A. Suppression of Listeria monocytogenes colonization following adsorption of nisin onto silica surfaces. Appl Environ Microbiol. 1995 Mar;61(3):992–997. doi: 10.1128/aem.61.3.992-997.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coventry M. J., Muirhead K., Hickey M. W. Partial characterisation of pediocin PO2 and comparison with nisin for biopreservation of meat products. Int J Food Microbiol. 1995 Jul;26(2):133–145. doi: 10.1016/0168-1605(94)00102-c. [DOI] [PubMed] [Google Scholar]
- Coventry M. J., Wan J., Gordon J. B., Mawson R. F., Hickey M. W. Production of brevicin 286 by Lactobacillus brevis VB286 and partial characterization. J Appl Bacteriol. 1996 Jan;80(1):91–98. doi: 10.1111/j.1365-2672.1996.tb03194.x. [DOI] [PubMed] [Google Scholar]
- Daba H., Lacroix C., Huang J., Simard R. E., Lemieux L. Simple method of purification and sequencing of a bacteriocin produced by Pediococcus acidilactici UL5. J Appl Bacteriol. 1994 Dec;77(6):682–688. doi: 10.1111/j.1365-2672.1994.tb02819.x. [DOI] [PubMed] [Google Scholar]
- Pillot J., Goueffon S., Keros R. G. Optimal conditions for elution of hepatitis B antigen after absorption onto colloidal silica. J Clin Microbiol. 1976 Sep;4(3):205–207. doi: 10.1128/jcm.4.3.205-207.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yang R., Johnson M. C., Ray B. Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Appl Environ Microbiol. 1992 Oct;58(10):3355–3359. doi: 10.1128/aem.58.10.3355-3359.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]