Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1996 May;62(5):1822–1824. doi: 10.1128/aem.62.5.1822-1824.1996

rpoS regulation of acid, heat, and salt tolerance in Escherichia coli O157:H7.

A M Cheville 1, K W Arnold 1, C Buchrieser 1, C M Cheng 1, C W Kaspar 1
PMCID: PMC167958  PMID: 8633882

Abstract

An rpoS mutant (rpoS::pRR10) of Escherichia coli O157:H7 ATCC 43895 was generated. Stationary-phase acid, heat, and salt tolerance was significantly reduced, and starvation-induced acid tolerance did not develop in the mutant. RpoS was also important for survival of E. coli O157:H7 in dry, fermented sausage.

Full Text

The Full Text of this article is available as a PDF (170.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnold K. W., Kaspar C. W. Starvation- and stationary-phase-induced acid tolerance in Escherichia coli O157:H7. Appl Environ Microbiol. 1995 May;61(5):2037–2039. doi: 10.1128/aem.61.5.2037-2039.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benjamin M. M., Datta A. R. Acid tolerance of enterohemorrhagic Escherichia coli. Appl Environ Microbiol. 1995 Apr;61(4):1669–1672. doi: 10.1128/aem.61.4.1669-1672.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Besser R. E., Lett S. M., Weber J. T., Doyle M. P., Barrett T. J., Wells J. G., Griffin P. M. An outbreak of diarrhea and hemolytic uremic syndrome from Escherichia coli O157:H7 in fresh-pressed apple cider. JAMA. 1993 May 5;269(17):2217–2220. [PubMed] [Google Scholar]
  4. Conner D. E., Kotrola J. S. Growth and survival of Escherichia coli O157:H7 under acidic conditions. Appl Environ Microbiol. 1995 Jan;61(1):382–385. doi: 10.1128/aem.61.1.382-385.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dassa J., Fsihi H., Marck C., Dion M., Kieffer-Bontemps M., Boquet P. L. A new oxygen-regulated operon in Escherichia coli comprises the genes for a putative third cytochrome oxidase and for pH 2.5 acid phosphatase (appA) Mol Gen Genet. 1991 Oct;229(3):341–352. doi: 10.1007/BF00267454. [DOI] [PubMed] [Google Scholar]
  6. Doyle M. P. Escherichia coli O157:H7 and its significance in foods. Int J Food Microbiol. 1991 Apr;12(4):289–301. doi: 10.1016/0168-1605(91)90143-d. [DOI] [PubMed] [Google Scholar]
  7. Fang F. C., Libby S. J., Buchmeier N. A., Loewen P. C., Switala J., Harwood J., Guiney D. G. The alternative sigma factor katF (rpoS) regulates Salmonella virulence. Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11978–11982. doi: 10.1073/pnas.89.24.11978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gorden J., Small P. L. Acid resistance in enteric bacteria. Infect Immun. 1993 Jan;61(1):364–367. doi: 10.1128/iai.61.1.364-367.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Griffin P. M., Tauxe R. V. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol Rev. 1991;13:60–98. doi: 10.1093/oxfordjournals.epirev.a036079. [DOI] [PubMed] [Google Scholar]
  10. Hengge-Aronis R. Survival of hunger and stress: the role of rpoS in early stationary phase gene regulation in E. coli. Cell. 1993 Jan 29;72(2):165–168. doi: 10.1016/0092-8674(93)90655-a. [DOI] [PubMed] [Google Scholar]
  11. Jenkins D. E., Chaisson S. A., Matin A. Starvation-induced cross protection against osmotic challenge in Escherichia coli. J Bacteriol. 1990 May;172(5):2779–2781. doi: 10.1128/jb.172.5.2779-2781.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jenkins D. E., Schultz J. E., Matin A. Starvation-induced cross protection against heat or H2O2 challenge in Escherichia coli. J Bacteriol. 1988 Sep;170(9):3910–3914. doi: 10.1128/jb.170.9.3910-3914.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lee I. S., Slonczewski J. L., Foster J. W. A low-pH-inducible, stationary-phase acid tolerance response in Salmonella typhimurium. J Bacteriol. 1994 Mar;176(5):1422–1426. doi: 10.1128/jb.176.5.1422-1426.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marugg J. D., van Spanje M., Hoekstra W. P., Schippers B., Weisbeek P. J. Isolation and analysis of genes involved in siderophore biosynthesis in plant-growth-stimulating Pseudomonas putida WCS358. J Bacteriol. 1985 Nov;164(2):563–570. doi: 10.1128/jb.164.2.563-570.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McCann M. P., Kidwell J. P., Matin A. The putative sigma factor KatF has a central role in development of starvation-mediated general resistance in Escherichia coli. J Bacteriol. 1991 Jul;173(13):4188–4194. doi: 10.1128/jb.173.13.4188-4194.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Morgan D., Newman C. P., Hutchinson D. N., Walker A. M., Rowe B., Majid F. Verotoxin producing Escherichia coli O 157 infections associated with the consumption of yoghurt. Epidemiol Infect. 1993 Oct;111(2):181–187. doi: 10.1017/s0950268800056880. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Morton D. S., Oliver J. D. Induction of Carbon Starvation-Induced Proteins in Vibrio vulnificus. Appl Environ Microbiol. 1994 Oct;60(10):3653–3659. doi: 10.1128/aem.60.10.3653-3659.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Munro P. M., Flatau G. N., Clément R. L., Gauthier M. J. Influence of the RpoS (KatF) sigma factor on maintenance of viability and culturability of Escherichia coli and Salmonella typhimurium in seawater. Appl Environ Microbiol. 1995 May;61(5):1853–1858. doi: 10.1128/aem.61.5.1853-1858.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Reeve C. A., Amy P. S., Matin A. Role of protein synthesis in the survival of carbon-starved Escherichia coli K-12. J Bacteriol. 1984 Dec;160(3):1041–1046. doi: 10.1128/jb.160.3.1041-1046.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Simon R., O'Connell M., Labes M., Pühler A. Plasmid vectors for the genetic analysis and manipulation of rhizobia and other gram-negative bacteria. Methods Enzymol. 1986;118:640–659. doi: 10.1016/0076-6879(86)18106-7. [DOI] [PubMed] [Google Scholar]
  21. Small P., Blankenhorn D., Welty D., Zinser E., Slonczewski J. L. Acid and base resistance in Escherichia coli and Shigella flexneri: role of rpoS and growth pH. J Bacteriol. 1994 Mar;176(6):1729–1737. doi: 10.1128/jb.176.6.1729-1737.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES