Abstract
Total DNAs isolated from two Hansenula polymorpha (Pichia angusta) strains having chromosomal single or tandem multiple integrations of a pUC18-derived expression plasmid produced Escherichia coli transformants which contained plasmids of different size and/or organization than that of the expression plasmid. Evidence that plasmid-like structures are formed in H. polymorpha and that their formation is stimulated by DNA damage is presented in this study.
Full Text
The Full Text of this article is available as a PDF (166.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bogdanova A. I., Agaphonov M. O., Ter-Avanesyan M. D. Plasmid reorganization during integrative transformation in Hansenula polymorpha. Yeast. 1995 Apr 15;11(4):343–353. doi: 10.1002/yea.320110407. [DOI] [PubMed] [Google Scholar]
- Gellissen G., Weydemann U., Strasser A. W., Piontek M., Janowicz Z. A., Hollenberg C. P. Progress in developing methylotrophic yeasts as expression systems. Trends Biotechnol. 1992 Dec;10(12):413–417. doi: 10.1016/0167-7799(92)90288-7. [DOI] [PubMed] [Google Scholar]
- Gilbert S. C., van Urk H., Greenfield A. J., McAvoy M. J., Denton K. A., Coghlan D., Jones G. D., Mead D. J. Increase in copy number of an integrated vector during continuous culture of Hansenula polymorpha expressing functional human haemoglobin. Yeast. 1994 Dec;10(12):1569–1580. doi: 10.1002/yea.320101206. [DOI] [PubMed] [Google Scholar]
- Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
- Jones R. S., Potter S. S. Characterization of cloned human alphoid satellite with an unusual monomeric construction: evidence for enrichment in HeLa small polydisperse circular DNA. Nucleic Acids Res. 1985 Feb 11;13(3):1027–1042. doi: 10.1093/nar/13.3.1027. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lorenz M. G., Wackernagel W. Bacterial gene transfer by natural genetic transformation in the environment. Microbiol Rev. 1994 Sep;58(3):563–602. doi: 10.1128/mr.58.3.563-602.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mazodier P., Davies J. Gene transfer between distantly related bacteria. Annu Rev Genet. 1991;25:147–171. doi: 10.1146/annurev.ge.25.120191.001051. [DOI] [PubMed] [Google Scholar]
- Prell A., Wackernagel W. Degradation of linear and circular DNA with gaps by the recBC enzyme of Escherichia coli. Effects of gap length and the presence of cell-free extracts. Eur J Biochem. 1980 Mar;105(1):109–116. doi: 10.1111/j.1432-1033.1980.tb04480.x. [DOI] [PubMed] [Google Scholar]
- Romanowski G., Lorenz M. G., Sayler G., Wackernagel W. Persistence of free plasmid DNA in soil monitored by various methods, including a transformation assay. Appl Environ Microbiol. 1992 Sep;58(9):3012–3019. doi: 10.1128/aem.58.9.3012-3019.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sunnerhagen P., Sjöberg R. M., Bjursell G. Increase of extrachromosomal circular DNA in mouse 3T6 cells on perturbation of DNA synthesis: implications for gene amplification. Somat Cell Mol Genet. 1989 Jan;15(1):61–70. doi: 10.1007/BF01534670. [DOI] [PubMed] [Google Scholar]
- Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
- Woodcock D. M., Crowther P. J., Doherty J., Jefferson S., DeCruz E., Noyer-Weidner M., Smith S. S., Michael M. Z., Graham M. W. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res. 1989 May 11;17(9):3469–3478. doi: 10.1093/nar/17.9.3469. [DOI] [PMC free article] [PubMed] [Google Scholar]