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. 1996 Jun;62(6):1885–1888. doi: 10.1128/aem.62.6.1885-1888.1996

Differential susceptibility of aeromonads and coliforms to cefsulodin.

J L Alonso 1, I Amoros 1, M A Alonso 1
PMCID: PMC167967  PMID: 8787387

Abstract

Cefsulodin was evaluated as a potential selective agent for aeromonads. Resistance of Aeromonas and coliform isolates was determined by using a standard disk diffusion technique. A total of 119 Aeromonas and 78 coliform strains were isolated. For 102 of 130 [corrected] Aeromonas isolates (environmental and reference strains), the MIC of cefsulodin was < 8 micrograms/ml. Results of MIC tests by the agar dilution method showed that a concentration of cefsulodin of 10 micrograms/ml or less inhibited the growth of 96% of isolates. In comparison, for 81 of 94 coliform isolates (environmental and reference strains), the MIC of cefsulodin was > 32 micrograms/ml. Because cefsulodin suppresses growth of Aeromonas and other oxidase-positive organisms, total coliform (TC) and Escherichia coli counts on Chromocult Coliform agar (CC agar) without cefsulodin and on CC agar with 10 mg of cefsulodin per liter (CC-CFS) were compared. Variance analysis of data from 14 sewage-polluted irrigation water specimens did not demonstrate any statistically significant difference in the enumeration of E. coli with CC and CC-CFS media. On average, the CC agar recovered 2.46 times as many TCs as CC-CFS. However, Aeromonas colonies made up an average of 58.6% of the TC counts on CC agar. Because no Aeromonas spp. were recovered on CC-CFS, background interference was eliminated and the counts that were obtained reflected more accurately the number of TCs. Results of this study suggest that cefsulodin may be a useful selective agent against Aeromonas spp. which should be included in coliform chromogenic media when high levels of accompanying flora are expected.

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Selected References

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  1. Altorfer R., Altwegg M., Zollinger-Iten J., von Graevenitz A. Growth of Aeromonas spp. on cefsulodin-Irgasan-novobiocin agar selective for Yersinia enterocolitica. J Clin Microbiol. 1985 Oct;22(4):478–480. doi: 10.1128/jcm.22.4.478-480.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brenner K. P., Rankin C. C., Roybal Y. R., Stelma G. N., Jr, Scarpino P. V., Dufour A. P. New medium for the simultaneous detection of total coliforms and Escherichia coli in water. Appl Environ Microbiol. 1993 Nov;59(11):3534–3544. doi: 10.1128/aem.59.11.3534-3544.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Edberg S. C., Allen M. J., Smith D. B. National field evaluation of a defined substrate method for the simultaneous enumeration of total coliforms and Escherichia coli from drinking water: comparison with the standard multiple tube fermentation method. Appl Environ Microbiol. 1988 Jun;54(6):1595–1601. doi: 10.1128/aem.54.6.1595-1601.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fainstein V., Weaver S., Bodey G. P. In vitro susceptibilities of Aeromonas hydrophila against new antibiotics. Antimicrob Agents Chemother. 1982 Sep;22(3):513–514. doi: 10.1128/aac.22.3.513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Freier T. A., Hartman P. A. Improved membrane filtration media for enumeration of total coliforms and Escherichia coli from sewage and surface waters. Appl Environ Microbiol. 1987 Jun;53(6):1246–1250. doi: 10.1128/aem.53.6.1246-1250.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grabow W. O., du Preez M. Comparison of m-Endo LES, MacConkey, and Teepol media for membrane filtration counting of total coliform bacteria in water. Appl Environ Microbiol. 1979 Sep;38(3):351–358. doi: 10.1128/aem.38.3.351-358.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Havelaar A. H., During M., Versteegh J. F. Ampicillin-dextrin agar medium for the enumeration of Aeromonas species in water by membrane filtration. J Appl Bacteriol. 1987 Mar;62(3):279–287. doi: 10.1111/j.1365-2672.1987.tb02410.x. [DOI] [PubMed] [Google Scholar]
  8. Janda J. M., Duffey P. S. Mesophilic aeromonads in human disease: current taxonomy, laboratory identification, and infectious disease spectrum. Rev Infect Dis. 1988 Sep-Oct;10(5):980–997. doi: 10.1093/clinids/10.5.980. [DOI] [PubMed] [Google Scholar]
  9. Kaper J. B., Lockman H., Colwell R. R., Joseph S. W. Aeromonas hydrophila: ecology and toxigenicity of isolates from an estuary. J Appl Bacteriol. 1981 Apr;50(2):359–377. doi: 10.1111/j.1365-2672.1981.tb00900.x. [DOI] [PubMed] [Google Scholar]
  10. Ley A., Barr S., Fredenburgh D., Taylor M., Walker N. Use of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside for the isolation of beta-galactosidase-positive bacteria from municipal water supplies. Can J Microbiol. 1993 Sep;39(9):821–825. doi: 10.1139/m93-122. [DOI] [PubMed] [Google Scholar]
  11. Manafi M., Kneifel W., Bascomb S. Fluorogenic and chromogenic substrates used in bacterial diagnostics. Microbiol Rev. 1991 Sep;55(3):335–348. doi: 10.1128/mr.55.3.335-348.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Moyer N. P., Geiss H. K., Marinescu M., Rigby A., Robinson J., Altwegg M. Media and methods for isolation of aeromonads from fecal specimens. A multilaboratory study. Experientia. 1991 May 15;47(5):409–412. [PubMed] [Google Scholar]
  13. Moyer N. P., Geiss H. K., Marinescu M., Rigby A., Robinson J., Altwegg M. Media and methods for isolation of aeromonads from fecal specimens. A multilaboratory study. Experientia. 1991 May 15;47(5):409–412. [PubMed] [Google Scholar]
  14. Nakano H., Kameyama T., Venkateswaran K., Kawakami H., Hashimoto H. Distribution and characterization of hemolytic, and enteropathogenic motile Aeromonas in aquatic environment. Microbiol Immunol. 1990;34(5):447–458. doi: 10.1111/j.1348-0421.1990.tb01027.x. [DOI] [PubMed] [Google Scholar]
  15. Qadri S. M., Billington O., Cunha B. A. In vitro activity of cefsulodin against multi-resistant isolates of Pseudomonas aeruginosa. Drugs Exp Clin Res. 1991;17(4):213–216. [PubMed] [Google Scholar]
  16. Rambach A. New plate medium for facilitated differentiation of Salmonella spp. from Proteus spp. and other enteric bacteria. Appl Environ Microbiol. 1990 Jan;56(1):301–303. doi: 10.1128/aem.56.1.301-303.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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