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. 1996 Jun;62(6):2174–2176. doi: 10.1128/aem.62.6.2174-2176.1996

New gene from nine Bacillus sphaericus strains encoding highly conserved 35.8-kilodalton mosquitocidal toxins.

J W Liu 1, A G Porter 1, B Y Wee 1, T Thanabalu 1
PMCID: PMC167996  PMID: 8787415

Abstract

A new gene encoding a 35.8-kDa mosquitocidal toxin (Mtx3; 326 amino acids) was isolated from Bacillus sphaericus SSII-1 DNA. Mtx3 is a new type of mosquitocidal toxin with homology to the Mtx2 mosquitocidal toxin of B. sphaericus SSII-1, the epsilon-toxin of Clostridium perfringens, and the cytotoxin of Pseudomonas aeruginosa. The mtx3 gene is highly conserved and widely distributed in both high- and low-toxicity mosquito larvicidal strains of B. sphaericus.

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Selected References

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  1. Baumann P., Clark M. A., Baumann L., Broadwell A. H. Bacillus sphaericus as a mosquito pathogen: properties of the organism and its toxins. Microbiol Rev. 1991 Sep;55(3):425–436. doi: 10.1128/mr.55.3.425-436.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bhown A. S., Habeerb A. F. Structural studies on epsilon-prototoxin of Clostridium perfringens type D. Localization of the site of tryptic scission necessary for activation to epsilon-toxin. Biochem Biophys Res Commun. 1977 Oct 10;78(3):889–896. doi: 10.1016/0006-291x(77)90506-x. [DOI] [PubMed] [Google Scholar]
  3. Chang S. Engineering for protein secretion in gram-positive bacteria. Methods Enzymol. 1987;153:507–516. doi: 10.1016/0076-6879(87)53075-0. [DOI] [PubMed] [Google Scholar]
  4. Hayashi T., Kamio Y., Hishinuma F., Usami Y., Titani K., Terawaki Y. Pseudomonas aeruginosa cytotoxin: the nucleotide sequence of the gene and the mechanism of activation of the protoxin. Mol Microbiol. 1989 Jul;3(7):861–868. doi: 10.1111/j.1365-2958.1989.tb00235.x. [DOI] [PubMed] [Google Scholar]
  5. Hunter S. E., Clarke I. N., Kelly D. C., Titball R. W. Cloning and nucleotide sequencing of the Clostridium perfringens epsilon-toxin gene and its expression in Escherichia coli. Infect Immun. 1992 Jan;60(1):102–110. doi: 10.1128/iai.60.1.102-110.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Liu J. W., Hindley J., Porter A. G., Priest F. G. New high-toxicity mosquitocidal strains of Bacillus sphaericus lacking a 100-kilodalton-toxin gene. Appl Environ Microbiol. 1993 Oct;59(10):3470–3473. doi: 10.1128/aem.59.10.3470-3473.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lutz F., Maurer M., Failing K. Cytotoxic protein from Pseudomonas aeruginosa: formation of hydrophilic pores in Ehrlich ascites tumor cells and effect on cell viability. Toxicon. 1987;25(3):293–305. doi: 10.1016/0041-0101(87)90258-3. [DOI] [PubMed] [Google Scholar]
  8. Moran C. P., Jr, Lang N., LeGrice S. F., Lee G., Stephens M., Sonenshein A. L., Pero J., Losick R. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Mol Gen Genet. 1982;186(3):339–346. doi: 10.1007/BF00729452. [DOI] [PubMed] [Google Scholar]
  9. Myers P., Yousten A. A., Davidson E. W. Comparative studies of the mosquito-larval toxin of Bacillus sphaericus SSII-1 and 1593. Can J Microbiol. 1979 Nov;25(11):1227–1231. doi: 10.1139/m79-193. [DOI] [PubMed] [Google Scholar]
  10. Porter A. G., Davidson E. W., Liu J. W. Mosquitocidal toxins of bacilli and their genetic manipulation for effective biological control of mosquitoes. Microbiol Rev. 1993 Dec;57(4):838–861. doi: 10.1128/mr.57.4.838-861.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rood J. I., Cole S. T. Molecular genetics and pathogenesis of Clostridium perfringens. Microbiol Rev. 1991 Dec;55(4):621–648. doi: 10.1128/mr.55.4.621-648.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith D. B., Johnson K. S. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 1988 Jul 15;67(1):31–40. doi: 10.1016/0378-1119(88)90005-4. [DOI] [PubMed] [Google Scholar]
  13. Thanabalu T., Hindley J., Berry C. Proteolytic processing of the mosquitocidal toxin from Bacillus sphaericus SSII-1. J Bacteriol. 1992 Aug;174(15):5051–5056. doi: 10.1128/jb.174.15.5051-5056.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Thanabalu T., Hindley J., Jackson-Yap J., Berry C. Cloning, sequencing, and expression of a gene encoding a 100-kilodalton mosquitocidal toxin from Bacillus sphaericus SSII-1. J Bacteriol. 1991 May;173(9):2776–2785. doi: 10.1128/jb.173.9.2776-2785.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thanabalu T., Porter A. G. A Bacillus sphaericus gene encoding a novel type of mosquitocidal toxin of 31.8 kDa. Gene. 1996 Apr 17;170(1):85–89. doi: 10.1016/0378-1119(95)00836-5. [DOI] [PubMed] [Google Scholar]

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