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. 1996 Jun;62(6):2191–2194. doi: 10.1128/aem.62.6.2191-2194.1996

Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus.

A Yamagishi 1, T Tanimoto 1, T Suzuki 1, T Oshima 1
PMCID: PMC167999  PMID: 8787418

Abstract

We have isolated uracil auxotrophic mutants of an extreme thermophile, Thermus thermophilus. A part of the pyrimidine biosynthetic operon including genes for orotate phosphoribosyltransferase (pyrE) and for orotidine-5'-monophosphate decarboxylase (pyrF) was cloned and sequenced. The pyrE gene can be a bidirectional marker for the gene manipulation system of the thermophile.

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Selected References

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