Abstract
The resistance to detergents and detergent-induced tolerance of a gastrointestinal organism, Enterococcus faecalis ATCC 19433, were examined. The most remarkable observation was the rapid response of cells in contact with bile salts and sodium dodecyl sulfate (SDS). The killing by high concentrations of detergents was nearly instantaneous. A 5-s adaptation with moderate sublethal concentrations of bile salts or SDS (0.08 or 0.01%, respectively) was sufficient to induce significant adaptation against homologous lethal conditions (0.3% bile salts or 0.017% SDS). However, resistance to a subsequent lethal challenge progressively increased further to a maximum reached after 30 min of adaptation. Furthermore, extremely strong cross-resistances were observed with bile salts- and SDS-adapted cells. However, no relationship seems to exist between levels of tolerance and de novo-synthesized proteins, since blockage of protein synthesis during adaptation had no effect on induction of resistance to bile salts and SDS. We conclude that this induced tolerance to detergent stress is independent of protein synthesis. Nevertheless, the stress-induced protein patterns of E. faecalis ATCC 19433 showed significant modifications. The rates of synthesis of 45 and 34 proteins were enhanced after treatments with bile salts and SDS, respectively. In spite of the overlap of 12 polypeptides, the protein profiles induced by the two detergents were different, suggesting that these detergents trigger different responses in E. faecalis. Therefore, bile salts cannot be substituted for SDS in biochemical detergent shock experiments with bacteria.
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- Adamowicz M., Kelley P. M., Nickerson K. W. Detergent (sodium dodecyl sulfate) shock proteins in Escherichia coli. J Bacteriol. 1991 Jan;173(1):229–233. doi: 10.1128/jb.173.1.229-233.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Aspedon A., Nickerson K. W. A two-part energy burden imposed by growth of Enterobacter cloacae and Escherichia coli in sodium dodecyl sulfate. Can J Microbiol. 1993 Jun;39(6):555–561. doi: 10.1139/m93-080. [DOI] [PubMed] [Google Scholar]
- Aspedon A., Nickerson K. W. The energy dependence of detergent resistance in Enterobacter cloacae: a likely requirement for ATP rather than a proton gradient or a membrane potential. Can J Microbiol. 1994 Mar;40(3):184–191. doi: 10.1139/m94-031. [DOI] [PubMed] [Google Scholar]
- Boutibonnes P., Giard J. C., Hartke A., Thammavongs B., Auffray Y. Characterization of the heat shock response in Enterococcus faecalis. Antonie Van Leeuwenhoek. 1993;64(1):47–55. doi: 10.1007/BF00870921. [DOI] [PubMed] [Google Scholar]
- Görg A., Postel W., Günther S. The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis. 1988 Sep;9(9):531–546. doi: 10.1002/elps.1150090913. [DOI] [PubMed] [Google Scholar]
- Kramer V. C., Nickerson K. W., Hamlett N. V., O'Hara C. Prevalence of extreme detergent resistance among the Enterobacteriaceae. Can J Microbiol. 1984 May;30(5):711–713. doi: 10.1139/m84-106. [DOI] [PubMed] [Google Scholar]
- Ma D., Cook D. N., Alberti M., Pon N. G., Nikaido H., Hearst J. E. Genes acrA and acrB encode a stress-induced efflux system of Escherichia coli. Mol Microbiol. 1995 Apr;16(1):45–55. doi: 10.1111/j.1365-2958.1995.tb02390.x. [DOI] [PubMed] [Google Scholar]
- Ma D., Cook D. N., Hearst J. E., Nikaido H. Efflux pumps and drug resistance in gram-negative bacteria. Trends Microbiol. 1994 Dec;2(12):489–493. doi: 10.1016/0966-842x(94)90654-8. [DOI] [PubMed] [Google Scholar]
- Nickerson K. W., Aspedon A. Detergent-shock response in enteric bacteria. Mol Microbiol. 1992 Apr;6(8):957–961. doi: 10.1111/j.1365-2958.1992.tb02161.x. [DOI] [PubMed] [Google Scholar]
- Nikaido H., Vaara M. Molecular basis of bacterial outer membrane permeability. Microbiol Rev. 1985 Mar;49(1):1–32. doi: 10.1128/mr.49.1.1-32.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
- Terzaghi B. E., Sandine W. E. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. doi: 10.1128/am.29.6.807-813.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]