Abstract
Most naturally occurring strains of the fungus Gibberella fujikuroi mating population A produce high levels of the mycotoxin fumonisin B1 (FB1), which is oxygenated at both carbons C-5 and C-10. Some strains, however, produce only FB2 or FB3, suggesting that they lack the ability to hydroxylate position C-10 or C-5, respectively. Genetic analysis indicates that these different phenotypes are due to single gene defects at closely linked loci designated fum2 and fum3. Further allellism tests indicate that both fum2 and fum3 are closely linked to fum1, a previously identified gene that regulates fumonisin production. The recovery frequency of FB1-producing progency from cross 510 between fum1 and fum2 mutations suggests a map distance of approximately 6.2 cM between these two loci. Amplified fragment length polymorphism analysis of parents and progeny of cross 510 was employed to confirm that the FB1-producing strains are recombinant progeny. We conclude that fum1, fum2, and fum3 constitute a fumonisin biosynthetic gene cluster on chromosome 1 of the restriction fragment length-map of G. fujikuroi.
Full Text
The Full Text of this article is available as a PDF (297.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Branham B. E., Plattner R. D. Alanine is a precursor in the biosynthesis of fumonisin B1 by Fusarium moniliforme. Mycopathologia. 1993 Nov;124(2):99–104. doi: 10.1007/BF01103109. [DOI] [PubMed] [Google Scholar]
- Brown D. W., Yu J. H., Kelkar H. S., Fernandes M., Nesbitt T. C., Keller N. P., Adams T. H., Leonard T. J. Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans. Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1418–1422. doi: 10.1073/pnas.93.4.1418. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Desjardins A. E., Hohn T. M., McCormick S. P. Trichothecene biosynthesis in Fusarium species: chemistry, genetics, and significance. Microbiol Rev. 1993 Sep;57(3):595–604. doi: 10.1128/mr.57.3.595-604.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Desjardins A. E., Plattner R. D., Proctor R. H. Genetic and biochemical aspects of fumonisin production. Adv Exp Med Biol. 1996;392:165–173. doi: 10.1007/978-1-4899-1379-1_15. [DOI] [PubMed] [Google Scholar]
- Desjardins A. E., Plattner R. D., Shackelford D. D., Leslie J. F., Nelson P. E. Heritability of fumonisin B1 production in Gibberella fujikuroi mating population A. Appl Environ Microbiol. 1992 Sep;58(9):2799–2805. doi: 10.1128/aem.58.9.2799-2805.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hohn T. M., Desjardins A. E. Isolation and gene disruption of the Tox5 gene encoding trichodiene synthase in Gibberella pulicaris. Mol Plant Microbe Interact. 1992 May-Jun;5(3):249–256. doi: 10.1094/mpmi-5-249. [DOI] [PubMed] [Google Scholar]
- Leslie J. F., Doe F. J., Plattner R. D., Shackelford D. D., Jonz J. Fumonisin B1 production and vegetative compatibility of strains from Gibberella fujikuroi mating population 'A' (Fusarium moniliforme). Mycopathologia. 1992 Feb;117(1-2):37–45. doi: 10.1007/BF00497277. [DOI] [PubMed] [Google Scholar]
- Nelson P. E., Plattner R. D., Shackelford D. D., Desjardins A. E. Production of fumonisins by Fusarium moniliforme strains from various substrates and geographic areas. Appl Environ Microbiol. 1991 Aug;57(8):2410–2412. doi: 10.1128/aem.57.8.2410-2412.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vos P., Hogers R., Bleeker M., Reijans M., van de Lee T., Hornes M., Frijters A., Pot J., Peleman J., Kuiper M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995 Nov 11;23(21):4407–4414. doi: 10.1093/nar/23.21.4407. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang E., Norred W. P., Bacon C. W., Riley R. T., Merrill A. H., Jr Inhibition of sphingolipid biosynthesis by fumonisins. Implications for diseases associated with Fusarium moniliforme. J Biol Chem. 1991 Aug 5;266(22):14486–14490. [PubMed] [Google Scholar]
- Xu J. R., Leslie J. F. A genetic map of Gibberella fujikuroi mating population A (Fusarium moniliforme). Genetics. 1996 May;143(1):175–189. doi: 10.1017/s0016672300034066. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yu J., Chang P. K., Cary J. W., Wright M., Bhatnagar D., Cleveland T. E., Payne G. A., Linz J. E. Comparative mapping of aflatoxin pathway gene clusters in Aspergillus parasiticus and Aspergillus flavus. Appl Environ Microbiol. 1995 Jun;61(6):2365–2371. doi: 10.1128/aem.61.6.2365-2371.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]