Abstract
The effects of conjugated bile acids, glycocholic acid, and taurocholic acid (TC) on production of thermostable direct hemolysin (TDH) by Vibrio parahaemolyticus were determined by a reversed passive latex agglutination assay against TDH. The amount of TDH excreted in growth medium containing either glycocholic acid or taurocholic acid (5 mM/liter) was, on a per-cell basis, 4- to 16-fold greater than that excreted in medium without the bile acids. The amounts of TDH released from lysed cells grown with the bile acids (5 mM/liter) were 4- to 32-fold greater than those from lysed cells grown without, suggesting that the bile acids enhanced synthesis of TDH within bacterial cells. These data imply that the conjugated bile acids play a key role in the pathogenicity of V. parahaemolyticus.
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- Barker W. H., Jr, Gangarosa E. J. Food poisoning due to Vibrio parahaemolyticus. Annu Rev Med. 1974;25:75–81. doi: 10.1146/annurev.me.25.020174.000451. [DOI] [PubMed] [Google Scholar]
- Blake P. A., Weaver R. E., Hollis D. G. Diseases of humans (other than cholera) caused by vibrios. Annu Rev Microbiol. 1980;34:341–367. doi: 10.1146/annurev.mi.34.100180.002013. [DOI] [PubMed] [Google Scholar]
- Cherwonogrodzky J. W., Skinner M. A., Clark A. G. Effect of D-tryptophan on hemolysin production in Vibrio parahaemolyticus. J Clin Microbiol. 1984 Nov;20(5):909–911. doi: 10.1128/jcm.20.5.909-911.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chun D., Chung J. K., Tak R., Seol S. Y. Nature of the Kanagawa phenomenon of Vibrio parahaemolyticus. Infect Immun. 1975 Jul;12(1):81–87. doi: 10.1128/iai.12.1.81-87.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fujisawa T., Mori M. Influence of bile salts on beta-glucuronidase activity of intestinal bacteria. Lett Appl Microbiol. 1996 Apr;22(4):271–274. doi: 10.1111/j.1472-765x.1996.tb01159.x. [DOI] [PubMed] [Google Scholar]
- Honda T., Goshima K., Takeda Y., Sugino Y., Miwatani T. Demonstration of the cardiotoxicity of the thermostable direct hemolysin (lethal toxin) produced by Vibrio parahaemolyticus. Infect Immun. 1976 Jan;13(1):163–171. doi: 10.1128/iai.13.1.163-171.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Honda T., Takeda Y., Miwatani T. Role of the cardiotoxin produced by Vibrio parahaemolyticus in its infection. Jpn J Med Sci Biol. 1977 Feb;30(1):84–86. [PubMed] [Google Scholar]
- Joseph S. W., Colwell R. R., Kaper J. B. Vibrio parahaemolyticus and related halophilic Vibrios. Crit Rev Microbiol. 1982;10(1):77–124. doi: 10.3109/10408418209113506. [DOI] [PubMed] [Google Scholar]
- Karunasagar I. Production of hemolysin by Vibrio parahaemolyticus in a chemically defined medium. Appl Environ Microbiol. 1981 May;41(5):1274–1275. doi: 10.1128/aem.41.5.1274-1275.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCarter L. L., Silverman M. Phosphate regulation of gene expression in Vibrio parahaemolyticus. J Bacteriol. 1987 Aug;169(8):3441–3449. doi: 10.1128/jb.169.8.3441-3449.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miyamoto Y., Kato T., Obara Y., Akiyama S., Takizawa K., Yamai S. In vitro hemolytic characteristic of Vibrio parahaemolyticus: its close correlation with human pathogenicity. J Bacteriol. 1969 Nov;100(2):1147–1149. doi: 10.1128/jb.100.2.1147-1149.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miyamoto Y., Obara Y., Nikkawa T., Yamai S., Kato T., Yamada Y., Ohashi M. Simplified purification and biophysicochemical characteristics of Kanagawa phenomenon-associated hemolysin of Vibrio parahaemolyticus. Infect Immun. 1980 May;28(2):567–576. doi: 10.1128/iai.28.2.567-576.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Noh D. O., Gilliland S. E. Influence of bile on cellular integrity and beta-galactosidase activity of Lactobacillus acidophilus. J Dairy Sci. 1993 May;76(5):1253–1259. doi: 10.3168/jds.S0022-0302(93)77454-8. [DOI] [PubMed] [Google Scholar]
- Ohta K., Kudoh Y., Tsuno M., Sakai S., Maruyama T., Itoh T., Ohashi M. [Development of a sensitive serological assay based on reversed passive hemagglutination for detection of enteropathogenic toxin (kanagawa hemolysin) of Vibrio parahaemolyticus, and re-evaluation of the toxin producibility of isolates from various sources (author's transl)]. Nihon Saikingaku Zasshi. 1979 Nov;34(6):837–846. [PubMed] [Google Scholar]
- Osawa R., Okitsu T., Morozumi H., Yamai S. Occurrence of urease-positive Vibrio parahaemolyticus in Kanagawa, Japan, with specific reference to presence of thermostable direct hemolysin (TDH) and the TDH-related-hemolysin genes. Appl Environ Microbiol. 1996 Feb;62(2):725–727. doi: 10.1128/aem.62.2.725-727.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sakazaki R., Iwanami S., Tamura K. Studies on the enteropathogenic, facultatively halophilic bacterium, Vibrio parahaemolyticus. II. Serological characteristics. Jpn J Med Sci Biol. 1968 Oct;21(5):313–324. doi: 10.7883/yoken1952.21.313. [DOI] [PubMed] [Google Scholar]