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. 1996 Aug;62(8):3042–3046. doi: 10.1128/aem.62.8.3042-3046.1996

Linear correlation between bacterial overexpression of recombinant peptides and cell light scatter.

F Lavergne-Mazeau 1, A Maftah 1, Y Cenatiempo 1, R Julien 1
PMCID: PMC168093  PMID: 8702299

Abstract

Fusion of multiple copies of a test peptide leads to insoluble inclusion bodies. Their presence within bacteria increases either forward-angle light scattering or, to a lesser extent, right-angle light scattering. A linear correlation has been established between cell forward-angle scattering and the level of overexpression of atrial natriuretic peptide. The correlation is valid only for unlysed cells and is protein product specific.

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Selected References

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