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. 1996 Sep;62(9):3360–3365. doi: 10.1128/aem.62.9.3360-3365.1996

Intraspecies variability of cellular fatty acids among soil and intestinal strains of Desulfovibrio desulfuricans.

Z Dzierzewicz 1, B Cwalina 1, S Kurkiewicz 1, E Chodurek 1, T Wilczok 1
PMCID: PMC168133  PMID: 8795227

Abstract

A comparison of cellular fatty acid profiles of Desulfovibrio desulfuricans DSM 642 and 14 wild strains of this species, isolated from two completely different environments, soil and the human intestine, was carried out. All the D. desulfuricans strains grown on lactate and sulfate indicated the presence of considerable amounts of i-C15:0, i-C17:1 and C16:0. Although differences in the quantities of individual fatty acids present in each strain were clear in the group of soil strains (similarity, 67.6%), in contrast to almost identical fatty acid patterns (similarity, near 100%) in the intestinal strains, the results were variable within the limits acceptable for species demonstration. The higher similarity of the fatty acid profiles of intestinal strains may be a result of the similarity of biocenoses in the human digestive tract. The coefficients of variability of i-C17:1 and i-C15:0 (the major branched-chain fatty acids), as well as clustering of the investigated strains compared with strains described in the literature after plotting percentages of i-C17:1 fatty acid against i-C15:0 fatty acid, confirmed a certain heterogeneity of cellular fatty acid profiles within the group of soil strains, in contrast to almost ideal homogeneity within the group of intestinal isolates. Intestinal strains contained a higher ratio of saturated to unsaturated fatty acids (2.2 +/- 0.14) than did soil strains (1.6 +/- 0.2; in one case, 2.7). We propose that intestinal D. desulfovibrio bacteria should be assumed to be a highly homogeneous group and should be represented by the strain D. desulfuricans subsp. intestinus in collections of microbial cultures.

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Selected References

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  1. Beerens H., Romond C. Sulfate-reducing anaerobic bacteria in human feces. Am J Clin Nutr. 1977 Nov;30(11):1770–1776. doi: 10.1093/ajcn/30.11.1770. [DOI] [PubMed] [Google Scholar]
  2. Boon J. J., de Leeuw J. W., Hoek G. J., Vosjan J. H. Significance and taxonomic value of iso and anteiso monoenoic fatty acids and branded beta-hydroxy acids in Desulfovibrio desulfuricans. J Bacteriol. 1977 Mar;129(3):1183–1191. doi: 10.1128/jb.129.3.1183-1191.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bruch R. C., Thayer W. S. Differential effect of lipid peroxidation on membrane fluidity as determined by electron spin resonance probes. Biochim Biophys Acta. 1983 Sep 7;733(2):216–222. doi: 10.1016/0005-2736(83)90525-4. [DOI] [PubMed] [Google Scholar]
  4. Christl S. U., Gibson G. R., Cummings J. H. Role of dietary sulphate in the regulation of methanogenesis in the human large intestine. Gut. 1992 Sep;33(9):1234–1238. doi: 10.1136/gut.33.9.1234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Devereux R., He S. H., Doyle C. L., Orkland S., Stahl D. A., LeGall J., Whitman W. B. Diversity and origin of Desulfovibrio species: phylogenetic definition of a family. J Bacteriol. 1990 Jul;172(7):3609–3619. doi: 10.1128/jb.172.7.3609-3619.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Florin T., Neale G., Gibson G. R., Christl S. U., Cummings J. H. Metabolism of dietary sulphate: absorption and excretion in humans. Gut. 1991 Jul;32(7):766–773. doi: 10.1136/gut.32.7.766. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fude L., Harris B., Urrutia M. M., Beveridge T. J. Reduction of Cr(VI) by a Consortium of Sulfate-Reducing Bacteria (SRB III). Appl Environ Microbiol. 1994 May;60(5):1525–1531. doi: 10.1128/aem.60.5.1525-1531.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fulco A. J. The biosynthesis of unsaturated fatty acids by bacilli. I. Temperature induction of the desaturation reaction. J Biol Chem. 1969 Feb 10;244(3):889–895. [PubMed] [Google Scholar]
  9. Gibson G. R., Cummings J. H., Macfarlane G. T. Competition for hydrogen between sulphate-reducing bacteria and methanogenic bacteria from the human large intestine. J Appl Bacteriol. 1988 Sep;65(3):241–247. doi: 10.1111/j.1365-2672.1988.tb01891.x. [DOI] [PubMed] [Google Scholar]
  10. Gibson G. R., Macfarlane G. T., Cummings J. H. Occurrence of sulphate-reducing bacteria in human faeces and the relationship of dissimilatory sulphate reduction to methanogenesis in the large gut. J Appl Bacteriol. 1988 Aug;65(2):103–111. doi: 10.1111/j.1365-2672.1988.tb01498.x. [DOI] [PubMed] [Google Scholar]
  11. Gibson G. R. Physiology and ecology of the sulphate-reducing bacteria. J Appl Bacteriol. 1990 Dec;69(6):769–797. doi: 10.1111/j.1365-2672.1990.tb01575.x. [DOI] [PubMed] [Google Scholar]
  12. Hamilton W. A. Sulphate-reducing bacteria and anaerobic corrosion. Annu Rev Microbiol. 1985;39:195–217. doi: 10.1146/annurev.mi.39.100185.001211. [DOI] [PubMed] [Google Scholar]
  13. Ilialetdinov A. N., Enker P. B., Loginova L. V. Uchastie sul'fatredutsiruiushchikh bakterii v osazhdenii medi. Mikrobiologiia. 1977 Jan-Feb;46(1):113–117. [PubMed] [Google Scholar]
  14. Jørgensen B. B. Ecology of the bacteria of the sulphur cycle with special reference to anoxic-oxic interface environments. Philos Trans R Soc Lond B Biol Sci. 1982 Sep 13;298(1093):543–561. doi: 10.1098/rstb.1982.0096. [DOI] [PubMed] [Google Scholar]
  15. Koga Y., Nishihara M., Morii H., Akagawa-Matsushita M. Ether polar lipids of methanogenic bacteria: structures, comparative aspects, and biosyntheses. Microbiol Rev. 1993 Mar;57(1):164–182. doi: 10.1128/mr.57.1.164-182.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Makula R. A., Finnerty W. R. Phospholipid composition of Desulfovibrio species. J Bacteriol. 1974 Dec;120(3):1279–1283. doi: 10.1128/jb.120.3.1279-1283.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moore L. V., Bourne D. M., Moore W. E. Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic gram-negative bacilli. Int J Syst Bacteriol. 1994 Apr;44(2):338–347. doi: 10.1099/00207713-44-2-338. [DOI] [PubMed] [Google Scholar]
  18. Petersen S. O., Klug M. J. Effects of sieving, storage, and incubation temperature on the phospholipid Fatty Acid profile of a soil microbial community. Appl Environ Microbiol. 1994 Jul;60(7):2421–2430. doi: 10.1128/aem.60.7.2421-2430.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]

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