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. 1996 Sep;62(9):3538–3540. doi: 10.1128/aem.62.9.3538-3540.1996

Increased mutagenesis mediated by cloned plasmid CAM-OCT genes: potential for expanding substrate ranges of Pseudomonas spp.

D L McBeth 1, B Hauer 1
PMCID: PMC168155  PMID: 8795249

Abstract

Twenty-five kilobases of Pseudomonas plasmid CAM-OCT DNA encoding a DNA repair gene(s) was cloned into the broad-host-range vector pVK100. The presence of the cloned genes increased the isolation frequency of Pseudomonas putida derivatives capable of using ethyl lactate or 3-methyl-3-buten-1-ol as their carbon source 15- and 8-fold, respectively, after UV irradiation. Ethyl lactate-utilizing strains expressed a novel intracellular hydrolase.

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Selected References

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