Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1996 Oct;62(10):3650–3654. doi: 10.1128/aem.62.10.3650-3654.1996

Kinetics of adhesion of selected fish-pathogenic Vibrio strains of skin mucus of gilt-head sea bream (Sparus aurata L.).

M A Bordas 1, M C Balebona 1, I Zorrilla 1, J J Borrego 1, M A Moriñigo 1
PMCID: PMC168172  PMID: 8837420

Abstract

The kinetics of adhesion of Vibrio strains isolated from diseased fish to skin mucus of gilt-head sea bream was studied. A modified Langmuir adsorption isotherm was calculated, and the results obtained indicate that the strains tested (Vibrio alginolyticus DP1HE4 and Vibrio anguillarum-like DC12R8 and DC12R9) showed a saturation kinetics except for V. alginolyticus (CAN), which showed a proportional adsorption kinetics. The adhesive capability for skin mucus does not seem to be an essential virulence factor of pathogenic strains of Vibrio, since this specific interaction depended on several environmental factors, temperature and salinity being the most important. However, the absence of an inhibitory effect of mucus on the pathogenic microorganisms, and the capability of the Vibrio strains to utilize mucus as a carbon source, could favor their settlement on the skin with a potential for infection of cultured, stressed fish.

Full Text

The Full Text of this article is available as a PDF (264.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appelbaum B., Golub E., Holt S. C., Rosan B. In vitro studies of dental plaque formation: adsorption of oral streptococci to hydroxyaptite. Infect Immun. 1979 Aug;25(2):717–728. doi: 10.1128/iai.25.2.717-728.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Attridge S. R., Rowley D. The role of the flagellum in the adherence of Vibrio cholerae. J Infect Dis. 1983 May;147(5):864–872. doi: 10.1093/infdis/147.5.864. [DOI] [PubMed] [Google Scholar]
  3. Beachey E. H. Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface. J Infect Dis. 1981 Mar;143(3):325–345. doi: 10.1093/infdis/143.3.325. [DOI] [PubMed] [Google Scholar]
  4. Belas M. R., Colwell R. R. Adsorption kinetics of laterally and polarly flagellated Vibrio. J Bacteriol. 1982 Sep;151(3):1568–1580. doi: 10.1128/jb.151.3.1568-1580.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gordon A. S., Millero F. J. Electrolyte effects on attachment of an estuarine bacterium. Appl Environ Microbiol. 1984 Mar;47(3):495–499. doi: 10.1128/aem.47.3.495-499.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grant C. C., Konkel M. E., Cieplak W., Jr, Tompkins L. S. Role of flagella in adherence, internalization, and translocation of Campylobacter jejuni in nonpolarized and polarized epithelial cell cultures. Infect Immun. 1993 May;61(5):1764–1771. doi: 10.1128/iai.61.5.1764-1771.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Göransson M., Sondén B., Nilsson P., Dagberg B., Forsman K., Emanuelsson K., Uhlin B. E. Transcriptional silencing and thermoregulation of gene expression in Escherichia coli. Nature. 1990 Apr 12;344(6267):682–685. doi: 10.1038/344682a0. [DOI] [PubMed] [Google Scholar]
  8. Hacker J. Role of fimbrial adhesins in the pathogenesis of Escherichia coli infections. Can J Microbiol. 1992 Jul;38(7):720–727. doi: 10.1139/m92-118. [DOI] [PubMed] [Google Scholar]
  9. Heimbrook M. E., Wang W. L., Campbell G. Staining bacterial flagella easily. J Clin Microbiol. 1989 Nov;27(11):2612–2615. doi: 10.1128/jcm.27.11.2612-2615.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hoepelman A. I., Tuomanen E. I. Consequences of microbial attachment: directing host cell functions with adhesins. Infect Immun. 1992 May;60(5):1729–1733. doi: 10.1128/iai.60.5.1729-1733.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jacques M., Kobisch M., Bélanger M., Dugal F. Virulence of capsulated and noncapsulated isolates of Pasteurella multocida and their adherence to porcine respiratory tract cells and mucus. Infect Immun. 1993 Nov;61(11):4785–4792. doi: 10.1128/iai.61.11.4785-4792.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jones B. D., Lee C. A., Falkow S. Invasion by Salmonella typhimurium is affected by the direction of flagellar rotation. Infect Immun. 1992 Jun;60(6):2475–2480. doi: 10.1128/iai.60.6.2475-2480.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Larsen J. L. Vibrio anguillarum: influence of temperature, pH, NaCl concentration and incubation time on growth. J Appl Bacteriol. 1984 Oct;57(2):237–246. doi: 10.1111/j.1365-2672.1984.tb01388.x. [DOI] [PubMed] [Google Scholar]
  14. Lindahl M., Faris A., Wadström T., Hjertén S. A new test based on 'salting out' to measure relative surface hydrophobicity of bacterial cells. Biochim Biophys Acta. 1981 Nov 5;677(3-4):471–476. doi: 10.1016/0304-4165(81)90261-0. [DOI] [PubMed] [Google Scholar]
  15. Mantle M., Rombough C. Growth in and breakdown of purified rabbit small intestinal mucin by Yersinia enterocolitica. Infect Immun. 1993 Oct;61(10):4131–4138. doi: 10.1128/iai.61.10.4131-4138.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Montgomery M. T., Kirchman D. L. Induction of Chitin-Binding Proteins during the Specific Attachment of the Marine Bacterium Vibrio harveyi to Chitin. Appl Environ Microbiol. 1994 Dec;60(12):4284–4288. doi: 10.1128/aem.60.12.4284-4288.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nachamkin I., Yang X. H., Stern N. J. Role of Campylobacter jejuni flagella as colonization factors for three-day-old chicks: analysis with flagellar mutants. Appl Environ Microbiol. 1993 May;59(5):1269–1273. doi: 10.1128/aem.59.5.1269-1273.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ogawa M., Ueda S., Ohto M., Kono N., Itami J., Kondo Y., Yamamoto S. Minimal change nephrotic syndrome developed after non-surgical treatment of a thymoma. Clin Nephrol. 1992 Sep;38(3):171–172. [PubMed] [Google Scholar]
  19. Piette J. P., Idziak E. S. Role of flagella in adhesion of Pseudomonas fluorescens to tendon slices. Appl Environ Microbiol. 1991 Jun;57(6):1635–1639. doi: 10.1128/aem.57.6.1635-1639.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Speare D. J., Mirsalimi S. M. Pathology of the mucous coat of trout skin during an erosive bacterial dermatitis: a technical advance in mucous coat stabilization for ultrastructural examination. J Comp Pathol. 1992 Apr;106(3):201–211. doi: 10.1016/0021-9975(92)90049-z. [DOI] [PubMed] [Google Scholar]
  21. Westerdahl A., Olsson J. C., Kjelleberg S., Conway P. L. Isolation and characterization of turbot (Scophtalmus maximus)-associated bacteria with inhibitory effects against Vibrio anguillarum. Appl Environ Microbiol. 1991 Aug;57(8):2223–2228. doi: 10.1128/aem.57.8.2223-2228.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zunino P., Piccini C., Legnani-Fajardo C. Flagellate and non-flagellate Proteus mirabilis in the development of experimental urinary tract infection. Microb Pathog. 1994 May;16(5):379–385. doi: 10.1006/mpat.1994.1038. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES