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. 1996 Oct;62(10):3800–3808. doi: 10.1128/aem.62.10.3800-3808.1996

Characterization of Desulfitobacterium chlororespirans sp. nov., which grows by coupling the oxidation of lactate to the reductive dechlorination of 3-chloro-4-hydroxybenzoate.

R A Sanford 1, J R Cole 1, F E Löffler 1, J M Tiedje 1
PMCID: PMC168189  PMID: 8837437

Abstract

Strain Co23, an anaerobic spore-forming microorganism, was enriched and isolated from a compost soil on the basis of its ability to grow with 2,3-dichlorophenol (DCP) as its electron acceptor, ortho chlorines were removed from polysubstituted phenols but not from monohalophenols. Growth by chlororespiration was indicated by a growth yield of 3.24 g of cells per mol of reducing equivalents (as 2[H]) from lactate oxidation to acetate in the presence of 3-chloro-4-hydroxybenzoate but no growth in the absence of the halogenated electron acceptor. Other indicators of chlororespiration were the fraction of electrons from the electron donor used for dechlorination (0.67) and the H2 threshold concentration of < 1.0 ppm. Additional electron donors utilized for reductive dehalogenation were pyruvate, formate, butyrate, crotonate, and H2. Pyruvate supported homoacetogenic growth in the absence of an electron acceptor. Strain Co23 also used sulfite, thiosulfate, and sulfur as electron acceptors for growth, but it did not use sulfate, nitrate or fumarate. The temperature optimum for growth was 37 degrees C; however, the rates of dechlorination were optimum at 45 degrees C and activity persisted to temperatures as high as 55 degrees C. The 16S rRNA sequence was determined, and strain Co23 was found to be related to Desulfitobacterium dehalogenans JW/IU DC1 and Desulfitobacterium strain PCE1, with sequence similarities of 97.2 and 96.8%, respectively. The phylogenetic and physiological properties exhibited by strain Co23 place it into a new species designated Desulfitobacterium chlororespirans.

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Selected References

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  1. Boyd S. A., Shelton D. R., Berry D., Tiedje J. M. Anaerobic biodegradation of phenolic compounds in digested sludge. Appl Environ Microbiol. 1983 Jul;46(1):50–54. doi: 10.1128/aem.46.1.50-54.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chaudhry G. R., Chapalamadugu S. Biodegradation of halogenated organic compounds. Microbiol Rev. 1991 Mar;55(1):59–79. doi: 10.1128/mr.55.1.59-79.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cole J. R., Cascarelli A. L., Mohn W. W., Tiedje J. M. Isolation and characterization of a novel bacterium growing via reductive dehalogenation of 2-chlorophenol. Appl Environ Microbiol. 1994 Oct;60(10):3536–3542. doi: 10.1128/aem.60.10.3536-3542.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DeWeerd K. A., Concannon F., Suflita J. M. Relationship between hydrogen consumption, dehalogenation, and the reduction of sulfur oxyanions by Desulfomonile tiedjei. Appl Environ Microbiol. 1991 Jul;57(7):1929–1934. doi: 10.1128/aem.57.7.1929-1934.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dolfing J. Reductive dechlorination of 3-chlorobenzoate is coupled to ATP production and growth in an anaerobic bacterium, strain DCB-1. Arch Microbiol. 1990;153(3):264–266. doi: 10.1007/BF00249079. [DOI] [PubMed] [Google Scholar]
  6. Genthner B. R., Price W. A., Pritchard P. H. Anaerobic Degradation of Chloroaromatic Compounds in Aquatic Sediments under a Variety of Enrichment Conditions. Appl Environ Microbiol. 1989 Jun;55(6):1466–1471. doi: 10.1128/aem.55.6.1466-1471.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gerritse J., Renard V., Pedro Gomes T. M., Lawson P. A., Collins M. D., Gottschal J. C. Desulfitobacterium sp. strain PCE1, an anaerobic bacterium that can grow by reductive dechlorination of tetrachloroethene or ortho-chlorinated phenols. Arch Microbiol. 1996 Feb;165(2):132–140. doi: 10.1007/s002030050308. [DOI] [PubMed] [Google Scholar]
  8. Gibson S. A., Suflita J. M. Extrapolation of biodegradation results to groundwater aquifers: reductive dehalogenation of aromatic compounds. Appl Environ Microbiol. 1986 Oct;52(4):681–688. doi: 10.1128/aem.52.4.681-688.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Guerrant G. O., Lambert M. A., Moss C. W. Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography. J Clin Microbiol. 1982 Aug;16(2):355–360. doi: 10.1128/jcm.16.2.355-360.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Holliger C., Schraa G., Stams A. J., Zehnder A. J. A highly purified enrichment culture couples the reductive dechlorination of tetrachloroethene to growth. Appl Environ Microbiol. 1993 Sep;59(9):2991–2997. doi: 10.1128/aem.59.9.2991-2997.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Häggblom M. M. Microbial breakdown of halogenated aromatic pesticides and related compounds. FEMS Microbiol Rev. 1992 Sep;9(1):29–71. doi: 10.1111/j.1574-6968.1992.tb05823.x. [DOI] [PubMed] [Google Scholar]
  12. Larsen N., Olsen G. J., Maidak B. L., McCaughey M. J., Overbeek R., Macke T. J., Marsh T. L., Woese C. R. The ribosomal database project. Nucleic Acids Res. 1993 Jul 1;21(13):3021–3023. doi: 10.1093/nar/21.13.3021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Li D. Y., Eberspächer J., Wagner B., Kuntzer J., Lingens F. Degradation of 2,4,6-trichlorophenol by Azotobacter sp. strain GP1. Appl Environ Microbiol. 1991 Jul;57(7):1920–1928. doi: 10.1128/aem.57.7.1920-1928.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Loffler F. E., Sanford R. A., Tiedje J. M. Initial Characterization of a Reductive Dehalogenase from Desulfitobacterium chlororespirans Co23. Appl Environ Microbiol. 1996 Oct;62(10):3809–3813. doi: 10.1128/aem.62.10.3809-3813.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Madsen T., Aamand H. Anaerobic transformation and toxicity of trichlorophenols in a stable enrichment culture. Appl Environ Microbiol. 1992 Feb;58(2):557–561. doi: 10.1128/aem.58.2.557-561.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Madsen T., Licht D. Isolation and characterization of an anaerobic chlorophenol-transforming bacterium. Appl Environ Microbiol. 1992 Sep;58(9):2874–2878. doi: 10.1128/aem.58.9.2874-2878.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mikesell M. D., Boyd S. A. Complete reductive dechlorination and mineralization of pentachlorophenol by anaerobic microorganisms. Appl Environ Microbiol. 1986 Oct;52(4):861–865. doi: 10.1128/aem.52.4.861-865.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mohn W. W., Tiedje J. M. Microbial reductive dehalogenation. Microbiol Rev. 1992 Sep;56(3):482–507. doi: 10.1128/mr.56.3.482-507.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mohn W. W., Tiedje J. M. Strain DCB-1 conserves energy for growth from reductive dechlorination coupled to formate oxidation. Arch Microbiol. 1990;153(3):267–271. doi: 10.1007/BF00249080. [DOI] [PubMed] [Google Scholar]
  20. Nicholson D. K., Woods S. L., Istok J. D., Peek D. C. Reductive dechlorination of chlorophenols by a pentachlorophenol- acclimated methanogenic consortium. Appl Environ Microbiol. 1992 Jul;58(7):2280–2286. doi: 10.1128/aem.58.7.2280-2286.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Olsen G. J., Matsuda H., Hagstrom R., Overbeek R. fastDNAmL: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci. 1994 Feb;10(1):41–48. doi: 10.1093/bioinformatics/10.1.41. [DOI] [PubMed] [Google Scholar]
  22. Shelton D. R., Tiedje J. M. Isolation and partial characterization of bacteria in an anaerobic consortium that mineralizes 3-chlorobenzoic Acid. Appl Environ Microbiol. 1984 Oct;48(4):840–848. doi: 10.1128/aem.48.4.840-848.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stanlake G. J., Finn R. K. Isolation and characterization of a pentachlorophenol-degrading bacterium. Appl Environ Microbiol. 1982 Dec;44(6):1421–1427. doi: 10.1128/aem.44.6.1421-1427.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Thauer R. K., Jungermann K., Decker K. Energy conservation in chemotrophic anaerobic bacteria. Bacteriol Rev. 1977 Mar;41(1):100–180. doi: 10.1128/br.41.1.100-180.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Utkin I., Dalton D. D., Wiegel J. Specificity of reductive dehalogenation of substituted ortho-chlorophenols by Desulfitobacterium dehalogenans JW/IU-DC1. Appl Environ Microbiol. 1995 Jan;61(1):346–351. doi: 10.1128/aem.61.1.346-351.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Utkin I., Woese C., Wiegel J. Isolation and characterization of Desulfitobacterium dehalogenans gen. nov., sp. nov., an anaerobic bacterium which reductively dechlorinates chlorophenolic compounds. Int J Syst Bacteriol. 1994 Oct;44(4):612–619. doi: 10.1099/00207713-44-4-612. [DOI] [PubMed] [Google Scholar]
  27. WOLIN E. A., WOLIN M. J., WOLFE R. S. FORMATION OF METHANE BY BACTERIAL EXTRACTS. J Biol Chem. 1963 Aug;238:2882–2886. [PubMed] [Google Scholar]
  28. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol. 1991 Jan;173(2):697–703. doi: 10.1128/jb.173.2.697-703.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Xun L., Topp E., Orser C. S. Purification and characterization of a tetrachloro-p-hydroquinone reductive dehalogenase from a Flavobacterium sp. J Bacteriol. 1992 Dec;174(24):8003–8007. doi: 10.1128/jb.174.24.8003-8007.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Zhang X., Wiegel J. Sequential anaerobic degradation of 2,4-dichlorophenol in freshwater sediments. Appl Environ Microbiol. 1990 Apr;56(4):1119–1127. doi: 10.1128/aem.56.4.1119-1127.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. de Jong E., Field J. A., Spinnler H. E., Wijnberg J. B., de Bont J. A. Significant biogenesis of chlorinated aromatics by fungi in natural environments. Appl Environ Microbiol. 1994 Jan;60(1):264–270. doi: 10.1128/aem.60.1.264-270.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]

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