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. 1996 Oct;62(10):3887–3889. doi: 10.1128/aem.62.10.3887-3889.1996

Prevalence of Lyme disease spirochetes in Ixodes persulcatus and wild rodents in far eastern Russia.

Y Sato 1, K Miyamoto 1, A Iwaki 1, T Masuzawa 1, Y Yanagihara 1, E I Korenberg 1, N B Gorelova 1, V I Volkov 1, L I Ivanov 1, R N Liberova 1
PMCID: PMC168201  PMID: 8837448

Abstract

Borrelia spirochetes were isolated from the adult ixodid tick (Ixodes persulcatus) in three areas of far eastern Russia, namely, Khabarovsk, Vladivostok, and Yuzhno-Sakhalinsk. Borrelia infective rates of ticks in those areas were 24.5, 41.4, and 25.1%, respectively (total rate was 26.6%). Spirochetes were also isolated from the tissues of small mammals captured at Khabarovsk (infective rate was 20.8%). Samples were classified by rRNA gene restriction fragment length polymorphism (RFLP) analysis. The isolated spirochetes from ticks were classified mainly RFLP ribotype group IV (B. garinii), followed by groups II (B. garinii), III (B. afzelii), and V (B. garinii), showing that B. garinii is a dominant species among them. Both B. garinii and B. afzelii were also found in rodents, and multiple infections with those two species were observed in some rodents. B. burgdorferi sensu stricto (group I) was not isolated from either ticks or rodents.

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Selected References

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  1. Ai C. X., Wen Y. X., Zhang Y. G., Wang S. S., Qiu Q. C., Shi Z. X., Li D. Y., Chen D. Q., Liu X. D., Zhao J. H. Clinical manifestations and epidemiological characteristics of Lyme disease in Hailin county, Heilongjiang Province, China. Ann N Y Acad Sci. 1988;539:302–313. doi: 10.1111/j.1749-6632.1988.tb31864.x. [DOI] [PubMed] [Google Scholar]
  2. Baranton G., Postic D., Saint Girons I., Boerlin P., Piffaretti J. C., Assous M., Grimont P. A. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. Int J Syst Bacteriol. 1992 Jul;42(3):378–383. doi: 10.1099/00207713-42-3-378. [DOI] [PubMed] [Google Scholar]
  3. Barbour A. G. Isolation and cultivation of Lyme disease spirochetes. Yale J Biol Med. 1984 Jul-Aug;57(4):521–525. [PMC free article] [PubMed] [Google Scholar]
  4. Fukunaga M., Koreki Y. A phylogenetic analysis of Borrelia burgdorferi sensu lato isolates associated with Lyme disease in Japan by flagellin gene sequence determination. Int J Syst Bacteriol. 1996 Apr;46(2):416–421. doi: 10.1099/00207713-46-2-416. [DOI] [PubMed] [Google Scholar]
  5. Fukunaga M., Sohnaka M., Yanagihara Y. Analysis of Borrelia species associated with Lyme disease by rRNA gene restriction fragment length polymorphism. J Gen Microbiol. 1993 Jun;139(Pt 6):1141–1146. doi: 10.1099/00221287-139-6-1141. [DOI] [PubMed] [Google Scholar]
  6. Kawabata M., Baba S., Iguchi K., Yamaguti N., Russell H. Lyme disease in Japan and its possible incriminated tick vector, Ixodes persulcatus. J Infect Dis. 1987 Nov;156(5):854–854. doi: 10.1093/infdis/156.5.854. [DOI] [PubMed] [Google Scholar]
  7. Korenberg E. I. Comparative ecology and epidemiology of lyme disease and tick-borne encephalitis in the former Soviet Union. Parasitol Today. 1994 Apr;10(4):157–160. doi: 10.1016/0169-4758(94)90269-0. [DOI] [PubMed] [Google Scholar]
  8. Korenberg E. I., Shcherbakov S. V., Zakharycheva T. A., Levin M. L., Kalinin M. I. Bolezn' Laima v Khabarovskom krae. Med Parazitol (Mosk) 1989 Sep-Oct;(5):74–78. [PubMed] [Google Scholar]
  9. Miyamoto K., Nakao M., Sato N., Mori M. Isolation of Lyme disease spirochetes from an ixodid tick in Hokkaido, Japan. Acta Trop. 1991 Apr;49(1):65–68. doi: 10.1016/0001-706x(91)90031-e. [DOI] [PubMed] [Google Scholar]
  10. Nakao M., Miyamoto K., Fukunaga M. Lyme disease spirochetes in Japan: enzootic transmission cycles in birds, rodents, and Ixodes persulcatus ticks. J Infect Dis. 1994 Oct;170(4):878–882. doi: 10.1093/infdis/170.4.878. [DOI] [PubMed] [Google Scholar]
  11. Nakao M., Miyamoto K. Mixed infection of different Borrelia species among Apodemus speciosus mice in Hokkaido, Japan. J Clin Microbiol. 1995 Feb;33(2):490–492. doi: 10.1128/jcm.33.2.490-492.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Park K. H., Chang W. H., Schwan T. G. Identification and characterization of Lyme disease spirochetes, Borrelia burgdorferi sensu lato, isolated in Korea. J Clin Microbiol. 1993 Jul;31(7):1831–1837. doi: 10.1128/jcm.31.7.1831-1837.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]

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