Abstract
Antisera were raised against nine strains which had been isolated from phenol-acclimated oil refinery activated sludge. Although several antisera reacted significantly with the activated sludge during a period of adaptation to phenol, only an antiserum against one of the isolates, Alcaligenes sp. E2, reacted with the activated sludge after the adaptation period. A kinetic pattern of phenol-oxygenating activity of the activated sludge after the adaptation period was similar to that of strain E2. These results suggest that a functionally important population in the phenol-digesting activated sludge was serologically identified.
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Selected References
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- Dwyer D. F., Krumme M. L., Boyd S. A., Tiedje J. M. Kinetics of phenol biodegradation by an immobilized methanogenic consortium. Appl Environ Microbiol. 1986 Aug;52(2):345–351. doi: 10.1128/aem.52.2.345-351.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Folsom B. R., Chapman P. J., Pritchard P. H. Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: kinetics and interactions between substrates. Appl Environ Microbiol. 1990 May;56(5):1279–1285. doi: 10.1128/aem.56.5.1279-1285.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harayama S., Kok M., Neidle E. L. Functional and evolutionary relationships among diverse oxygenases. Annu Rev Microbiol. 1992;46:565–601. doi: 10.1146/annurev.mi.46.100192.003025. [DOI] [PubMed] [Google Scholar]
- Hiraishi A., Masamune K., Kitamura H. Characterization of the bacterial population structure in an anaerobic-aerobic activated sludge system on the basis of respiratory quinone profiles. Appl Environ Microbiol. 1989 Apr;55(4):897–901. doi: 10.1128/aem.55.4.897-901.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Macario A. J., Conway de Macario E. Quantitative immunologic analysis of the methanogenic flora of digestors reveals a considerable diversity. Appl Environ Microbiol. 1988 Jan;54(1):79–86. doi: 10.1128/aem.54.1.79-86.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Molin G., Nilsson I. Degradation of phenol by Pseudomonas putida ATCC 11172 in continuous culture at different ratios of biofilm surface to culture volume. Appl Environ Microbiol. 1985 Oct;50(4):946–950. doi: 10.1128/aem.50.4.946-950.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saraswat N., Alleman J. E., Smith T. J. Enzyme Immunoassay Detection of Nitrosomonas europaea. Appl Environ Microbiol. 1994 Jun;60(6):1969–1973. doi: 10.1128/aem.60.6.1969-1973.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sayler G. S., Layton A. C. Environmental application of nucleic acid hybridization. Annu Rev Microbiol. 1990;44:625–648. doi: 10.1146/annurev.mi.44.100190.003205. [DOI] [PubMed] [Google Scholar]
- Völsch A., Nader W. F., Geiss H. K., Nebe G., Birr C. Detection and analysis of two serotypes of ammonia-oxidizing bacteria in sewage plants by flow cytometry. Appl Environ Microbiol. 1990 Aug;56(8):2430–2435. doi: 10.1128/aem.56.8.2430-2435.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wagner M., Amann R., Lemmer H., Schleifer K. H. Probing activated sludge with oligonucleotides specific for proteobacteria: inadequacy of culture-dependent methods for describing microbial community structure. Appl Environ Microbiol. 1993 May;59(5):1520–1525. doi: 10.1128/aem.59.5.1520-1525.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]