Abstract
A new bioreactor for the removal of nitrogen from wastewater is described which consists of a tubular polymeric gel containing Nitrosomonas europaea and Paracoccus denitrificans. The outer surface of the tube is in aerobic contact with wastewater containing ammonia, while the inside of the tube is in anaerobic contact with ethanol flowing through the tube. N. europaea oxidizes ammonia to nitrite in the gel, and then P. denitrificans reduces the nitrite to nitrogen gas in the same gel. This concept would be effective for simplifying nitrogen removal systems requiring aerobic and anaerobic operations.
Full Text
The Full Text of this article is available as a PDF (354.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson I. C., Poth M., Homstead J., Burdige D. A comparison of NO and N2O production by the autotrophic nitrifier Nitrosomonas europaea and the heterotrophic nitrifier Alcaligenes faecalis. Appl Environ Microbiol. 1993 Nov;59(11):3525–3533. doi: 10.1128/aem.59.11.3525-3533.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blackburn T. H., Blackburn N. D., Jensen K., Risgaard-Petersen N. Simulation Model of the Coupling between Nitrification and Denitrification in a Freshwater Sediment. Appl Environ Microbiol. 1994 Sep;60(9):3089–3095. doi: 10.1128/aem.60.9.3089-3095.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hooper A. B., Terry K. R. Photoinactivation of ammonia oxidation in Nitrosomonas. J Bacteriol. 1974 Sep;119(3):899–906. doi: 10.1128/jb.119.3.899-906.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hunik J. H., van den Hoogen M. P., de Boer W., Smit M., Tramper J. Quantitative Determination of the Spatial Distribution of Nitrosomonas europaea and Nitrobacter agilis Cells Immobilized in kappa-Carrageenan Gel Beads by a Specific Fluorescent-Antibody Labelling Technique. Appl Environ Microbiol. 1993 Jun;59(6):1951–1954. doi: 10.1128/aem.59.6.1951-1954.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jensen K., Sloth N. P., Risgaard-Petersen N., Rysgaard S., Revsbech N. P. Estimation of nitrification and denitrification from microprofiles of oxygen and nitrate in model sediment systems. Appl Environ Microbiol. 1994 Jun;60(6):2094–2100. doi: 10.1128/aem.60.6.2094-2100.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Körner H., Zumft W. G. Expression of denitrification enzymes in response to the dissolved oxygen level and respiratory substrate in continuous culture of Pseudomonas stutzeri. Appl Environ Microbiol. 1989 Jul;55(7):1670–1676. doi: 10.1128/aem.55.7.1670-1676.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Poth M., Focht D. D. N Kinetic Analysis of N(2)O Production by Nitrosomonas europaea: an Examination of Nitrifier Denitrification. Appl Environ Microbiol. 1985 May;49(5):1134–1141. doi: 10.1128/aem.49.5.1134-1141.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rysgaard S., Risgaard-Petersen N., Nielsen L. P., Revsbech N. P. Nitrification and denitrification in lake and estuarine sediments measured by the N dilution technique and isotope pairing. Appl Environ Microbiol. 1993 Jul;59(7):2093–2098. doi: 10.1128/aem.59.7.2093-2098.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Santos V. A., Tramper J., Wijffels R. H. Simultaneous nitrification and denitrification using immobilized microorganisms. Biomater Artif Cells Immobilization Biotechnol. 1993;21(3):317–322. doi: 10.3109/10731199309117369. [DOI] [PubMed] [Google Scholar]
- Schmidt E. L., Bakole R. O., Bohlool B. B. Fluorescent-antibody approach to study of rhizobia in soil. J Bacteriol. 1968 Jun;95(6):1987–1992. doi: 10.1128/jb.95.6.1987-1992.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Soriano S., Walker N. Isolation of ammonia-oxidizing autotrophic bacteria. J Appl Bacteriol. 1968 Dec;31(4):493–497. doi: 10.1111/j.1365-2672.1968.tb00397.x. [DOI] [PubMed] [Google Scholar]