Abstract
The ability of a microbial consortium eluted from dioxin-contaminated Passaic River sediments to dechlorinate polychlorinated dibenzo-p-dioxins (PCDDs) was investigated under methanogenic conditions. Aged 2,3,7,8-tetraCDD, which had partitioned into the microbial consortium from sediments, was stoichiometrically converted to tri- and monoCDD congeners. During dechlorination, dominant microbial activity within the consortium shifted from methanogenic to nonmethanogenic activity. Freshly spiked octaCDD was converted to hepta-, hexa-, penta-, tetra-, tri-, di-, and monochlorinated isomers, but the reaction stoichiometry was not determined. No methanogenic activity was observed, and the maximum yield of protein coincided with the production of less-chlorinated DD congeners. Two distinct pathways of dechlorination were observed: the peri-dechlorination pathway of 2,3,7,8-substituted hepta- to pentaCDDs, resulting in the production of 2,3,7,8-tetraCDD, and the peri-lateral dechlorination pathway of non-2,3,7,8-substituted congeners. Direct evidence of further lateral dechlorination of 2,3,7,8-tetraCDD was obtained from the historically contaminated incubations; no isomer-specific identification of triCDDs in spiked incubations was determined. Pasteurized cells exhibited no peri-dechlorination pathway, and triCDDs were the least-chlorinated congeners produced in these treatments. These results demonstrate that (i) both freshly spiked and aged PCDDs are available to microbial reductive dechlorination, (ii) the peri and triCDD dechlorinations are attributed to activities of nonmethanogenic, non-spore-forming microbial subpopulations, and (iii) the 2,3,7,8-residue patterns in historically contaminated sediments are likely affected by microbial activity.
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- Ascon-Cabrera M., Lebeault J. M. Selection of xenobiotic-degrading microorganisms in a biphasic aqueous-organic system. Appl Environ Microbiol. 1993 Jun;59(6):1717–1724. doi: 10.1128/aem.59.6.1717-1724.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baughman G. L., Paris D. F. Microbial bioconcentration of organic pollutants from aquatic systems -- a critical review. Crit Rev Microbiol. 1981;8(3):205–228. doi: 10.3109/10408418109085079. [DOI] [PubMed] [Google Scholar]
- Boyle A. W., Blake C. K., Price W. A., May H. D. Effects of polychlorinated biphenyl congener concentration and sediment supplementation on rates of methanogenesis and 2,3,6-trichlorobiphenyl dechlorination in an anaerobic enrichment. Appl Environ Microbiol. 1993 Sep;59(9):3027–3031. doi: 10.1128/aem.59.9.3027-3031.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DiStefano T. D., Gossett J. M., Zinder S. H. Reductive dechlorination of high concentrations of tetrachloroethene to ethene by an anaerobic enrichment culture in the absence of methanogenesis. Appl Environ Microbiol. 1991 Aug;57(8):2287–2292. doi: 10.1128/aem.57.8.2287-2292.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heier L. A., Carpanzano C. R., Mast J., Brill P. W., Winchester P., Deck M. D. Maternal cocaine abuse: the spectrum of radiologic abnormalities in the neonatal CNS. AJNR Am J Neuroradiol. 1991 Sep-Oct;12(5):951–956. [PMC free article] [PubMed] [Google Scholar]
- Heijman C. G., Holliger C., Glaus M. A., Schwarzenbach R. P., Zeyer J. Abiotic reduction of 4-chloronitrobenzene to 4-chloroaniline in a dissimilatory iron-reducing enrichment culture. Appl Environ Microbiol. 1993 Dec;59(12):4350–4353. doi: 10.1128/aem.59.12.4350-4353.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holliger C., Schraa G. Physiological meaning and potential for application of reductive dechlorination by anaerobic bacteria. FEMS Microbiol Rev. 1994 Oct;15(2-3):297–305. doi: 10.1111/j.1574-6976.1994.tb00141.x. [DOI] [PubMed] [Google Scholar]
- Poland A., Knutson J. C. 2,3,7,8-tetrachlorodibenzo-p-dioxin and related halogenated aromatic hydrocarbons: examination of the mechanism of toxicity. Annu Rev Pharmacol Toxicol. 1982;22:517–554. doi: 10.1146/annurev.pa.22.040182.002505. [DOI] [PubMed] [Google Scholar]
- Van Dort H. M., Bedard D. L. Reductive ortho and meta Dechlorination of a Polychlorinated Biphenyl Congener by Anaerobic Microorganisms. Appl Environ Microbiol. 1991 May;57(5):1576–1578. doi: 10.1128/aem.57.5.1576-1578.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wenning R. J., Harris M. A., Finley B., Paustenbach D. J., Bedbury H. Application of pattern recognition techniques to evaluate polychlorinated dibenzo-p-dioxin and dibenzofuran distributions in surficial sediments from the lower Passaic River and Newark Bay. Ecotoxicol Environ Saf. 1993 Feb;25(1):103–125. doi: 10.1006/eesa.1993.1011. [DOI] [PubMed] [Google Scholar]
- Wolfaardt G. M., Lawrence J. R., Robarts R. D., Caldwell D. E. Bioaccumulation of the Herbicide Diclofop in Extracellular Polymers and Its Utilization by a Biofilm Community during Starvation. Appl Environ Microbiol. 1995 Jan;61(1):152–158. doi: 10.1128/aem.61.1.152-158.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ye D., Quensen J. F., 3rd, Tiedje J. M., Boyd S. A. Anaerobic dechlorination of polychlorobiphenyls (Aroclor 1242) by pasteurized and ethanol-treated microorganisms from sediments. Appl Environ Microbiol. 1992 Apr;58(4):1110–1114. doi: 10.1128/aem.58.4.1110-1114.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ye D., Quensen J. I., Tiedje J. M., Boyd S. A. Evidence for para dechlorination of polychlorobiphenyls by methanogenic bacteria. Appl Environ Microbiol. 1995 Jun;61(6):2166–2171. doi: 10.1128/aem.61.6.2166-2171.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]