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. 1996 Dec;62(12):4614–4620. doi: 10.1128/aem.62.12.4614-4620.1996

Microbial ecology of Campylobacter jejuni in a United Kingdom chicken supply chain: intermittent common source, vertical transmission, and amplification by flock propagation.

A D Pearson 1, M H Greenwood 1, R K Feltham 1, T D Healing 1, J Donaldson 1, D M Jones 1, R R Colwell 1
PMCID: PMC168286  PMID: 8953731

Abstract

A study of Campylobacter jejuni on a broiler chicken farm between 1989 and 1994 gave an estimated isolation rate of 27% (3,304 of 12,233) from a 0.9% sample of 1.44 million broiler chickens from six to eight sheds over 32 consecutive rearing flocks comprising 251 broiler shed flocks. During the study, C. jejuni was found in 35.5% of the 251 shed flocks but only 9.2% (23 of 251) had Campylobacter isolates in successive flocks, with 9 of those 23 sheds having the same serotype between consecutive flocks, indicating a low level of transmission between flocks. Analysis of a systematic sample of 484 of 3,304 (14.6%) C. jejuni isolates showed that 85% were of 10 serotype complexes but 58% were of 3 serotype complexes, indicating a high degree of strain similarity throughout the entire study. The three commonest types were detected in 8 of 32 flocks during the 5-year study period, suggesting an intermittent common external Campylobacter source. This hypothesis was tested by a retrospective cohort analysis of C. jejuni rates and types by reference to hatchery supplier of the 1-day-old chicks. Isolation rates of C. jejuni and frequency distribution of types were determined in 6-week-old broiler chickens identified by the hatchery supplying the original chicks. The isolation rate of C. jejuni in broilers, supplied by hatchery A, was 17.6%, compared to 42.9% (P < 0.0001) for broilers reared from chicks supplied by hatchery B. In two instances, when both hatcheries were used to stock the same farm flock, Campylobacter isolates were found only in those sheds with chicks supplied by hatchery B. Thus, the frequency distribution of Campylobacter types for chickens supplied by the two hatcheries over the 5-year period showed marked dissimilarity. These findings suggest that the isolation rate and type of Campylobacter isolates in broiler chickens was associated with the hatchery supplying chicks. The lack of diversity of types and the intermittent high positivity of sheds is evidence for a common source of C. jejuni introduced by vertical transmission rather than contamination at the hatchery or during transportation.

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Selected References

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  1. Adak G. K., Cowden J. M., Nicholas S., Evans H. S. The Public Health Laboratory Service national case-control study of primary indigenous sporadic cases of campylobacter infection. Epidemiol Infect. 1995 Aug;115(1):15–22. doi: 10.1017/s0950268800058076. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beery J. T., Hugdahl M. B., Doyle M. P. Colonization of gastrointestinal tracts of chicks by Campylobacter jejuni. Appl Environ Microbiol. 1988 Oct;54(10):2365–2370. doi: 10.1128/aem.54.10.2365-2370.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bolton F. J., Coates D., Hinchliffe P. M., Robertson L. Comparison of selective media for isolation of Campylobacter jejuni/coli. J Clin Pathol. 1983 Jan;36(1):78–83. doi: 10.1136/jcp.36.1.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bolton F. J., Holt A. V., Hutchinson D. N. Campylobacter biotyping scheme of epidemiological value. J Clin Pathol. 1984 Jun;37(6):677–681. doi: 10.1136/jcp.37.6.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bolton F. J., Robertson L. A selective medium for isolating Campylobacter jejuni/coli. J Clin Pathol. 1982 Apr;35(4):462–467. doi: 10.1136/jcp.35.4.462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Deming M. S., Tauxe R. V., Blake P. A., Dixon S. E., Fowler B. S., Jones T. S., Lockamy E. A., Patton C. M., Sikes R. O. Campylobacter enteritis at a university: transmission from eating chicken and from cats. Am J Epidemiol. 1987 Sep;126(3):526–534. doi: 10.1093/oxfordjournals.aje.a114685. [DOI] [PubMed] [Google Scholar]
  7. Doyle M. P. Association of Campylobacter jejuni with laying hens and eggs. Appl Environ Microbiol. 1984 Mar;47(3):533–536. doi: 10.1128/aem.47.3.533-536.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Evans S. J. Introduction and spread of thermophilic campylobacters in broiler flocks. Vet Rec. 1992 Dec 19;131(25-26):574–576. [PubMed] [Google Scholar]
  9. Finch M. J., Blake P. A. Foodborne outbreaks of campylobacteriosis: the United States experience, 1980-1982. Am J Epidemiol. 1985 Aug;122(2):262–268. doi: 10.1093/oxfordjournals.aje.a114097. [DOI] [PubMed] [Google Scholar]
  10. Humphrey T. J., Henley A., Lanning D. G. The colonization of broiler chickens with Campylobacter jejuni: some epidemiological investigations. Epidemiol Infect. 1993 Jun;110(3):601–607. doi: 10.1017/s0950268800051025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jacobs-Reitsma W. F., van de Giessen A. W., Bolder N. M., Mulder R. W. Epidemiology of Campylobacter spp. at two Dutch broiler farms. Epidemiol Infect. 1995 Jun;114(3):413–421. doi: 10.1017/s0950268800052122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kapperud G., Skjerve E., Bean N. H., Ostroff S. M., Lassen J. Risk factors for sporadic Campylobacter infections: results of a case-control study in southeastern Norway. J Clin Microbiol. 1992 Dec;30(12):3117–3121. doi: 10.1128/jcm.30.12.3117-3121.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kapperud G., Skjerve E., Vik L., Hauge K., Lysaker A., Aalmen I., Ostroff S. M., Potter M. Epidemiological investigation of risk factors for campylobacter colonization in Norwegian broiler flocks. Epidemiol Infect. 1993 Oct;111(2):245–255. doi: 10.1017/s0950268800056958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. King V., Bavetsia A., Bumstead N. Effect of host lineage on the virulence of Campylobacter jejuni/coli in the chick embryo model. FEMS Microbiol Lett. 1993 Feb 1;106(3):271–274. doi: 10.1111/j.1574-6968.1993.tb05975.x. [DOI] [PubMed] [Google Scholar]
  15. Kuroki S., Haruta T., Yoshioka M., Kobayashi Y., Nukina M., Nakanishi H. Guillain-Barré syndrome associated with Campylobacter infection. Pediatr Infect Dis J. 1991 Feb;10(2):149–151. doi: 10.1097/00006454-199102000-00014. [DOI] [PubMed] [Google Scholar]
  16. Munroe D. L., Prescott J. F., Penner J. L. Campylobacter jejuni and Campylobacter coli serotypes isolated from chickens, cattle, and pigs. J Clin Microbiol. 1983 Oct;18(4):877–881. doi: 10.1128/jcm.18.4.877-881.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pearson A. D., Greenwood M., Healing T. D., Rollins D., Shahamat M., Donaldson J., Colwell R. R. Colonization of broiler chickens by waterborne Campylobacter jejuni. Appl Environ Microbiol. 1993 Apr;59(4):987–996. doi: 10.1128/aem.59.4.987-996.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Penner J. L., Hennessy J. N. Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. J Clin Microbiol. 1980 Dec;12(6):732–737. doi: 10.1128/jcm.12.6.732-737.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sanyal S. C., Islam K. M., Neogy P. K., Islam M., Speelman P., Huq M. I. Campylobacter jejuni diarrhea model in infant chickens. Infect Immun. 1984 Mar;43(3):931–936. doi: 10.1128/iai.43.3.931-936.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shane S. M., Gifford D. H., Yogasundram K. Campylobacter jejuni contamination of eggs. Vet Res Commun. 1986 Nov;10(6):487–492. doi: 10.1007/BF02214012. [DOI] [PubMed] [Google Scholar]
  21. Shanker S., Lee A., Sorrell T. C. Campylobacter jejuni in broilers: the role of vertical transmission. J Hyg (Lond) 1986 Apr;96(2):153–159. doi: 10.1017/s002217240006592x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shanker S., Lee A., Sorrell T. C. Horizontal transmission of Campylobacter jejuni amongst broiler chicks: experimental studies. Epidemiol Infect. 1990 Feb;104(1):101–110. doi: 10.1017/s0950268800054571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. van de Giessen A., Mazurier S. I., Jacobs-Reitsma W., Jansen W., Berkers P., Ritmeester W., Wernars K. Study on the epidemiology and control of Campylobacter jejuni in poultry broiler flocks. Appl Environ Microbiol. 1992 Jun;58(6):1913–1917. doi: 10.1128/aem.58.6.1913-1917.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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