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. 1991 Feb;48(2):325–334.

Saturating the region of the polycystic kidney disease gene with NotI linking clones.

H Himmelbauer 1, G G Germino 1, I Ceccherini 1, G Romeo 1, S T Reeders 1, A M Frischauf 1
PMCID: PMC1683004  PMID: 1990840

Abstract

A NotI-linking library was constructed from a radiation hybrid containing fragments of human chromosome 16. The clones were mapped on a panel of somatic cell hybrids, and 10 different NotI site-containing clones were localized close to and between genetic markers flanking the PKD1 locus. With pulsed-field gel analysis the clones were shown to be distributed over four adjacent ClaI fragments covering 1,200 kb.

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Selected References

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