Abstract
Microbial toxins and eukaryotic cell toxicity from indoor building materials heavily colonized by fungi and bacteria were analyzed. The dominant colonizers at water-damaged sites of the building were Stachybotrys chartarum (10(3) to 10(5) visible conidia cm-2), Penicillium and Aspergillus species (10(4) CFU mg-1), gram-negative bacteria (10(4) CFU mg-1), and mycobacteria (10(3) CFU mg-1). The mycobacterial isolates were most similar to M. komossense, with 98% similarity of the complete 16S rDNA sequence. Limulus assay of water extracts prepared from a water-damaged gypsum liner revealed high contents of gram-negative endotoxin (17 ng mg-1 of E. coli lipopolysaccharide equivalents) and beta-D-glucan (210 ng mg-1 of curdlan equivalents). High-performance liquid chromatography analysis of the methanol extracts showed that the water-damaged gypsum liner also contained satratoxin (17 ng mg-1). This methanol-extracted substance was 200 times more toxic to rabbit skin and fetus feline lung cells than extract of gypsum liner sampled from a non-water-damaged site. The same extract contained toxin(s) that paralyzed the motility of boar spermatozoa at extremely low concentrations; the 50% effective concentration was 0.3 microgram of dry solids per ml. This toxicity was not explainable by the amount of bacterial endotoxin, beta-D-glucan, or satratoxin present in the same extract. The novel in vitro toxicity test that utilized boar spermatozoa as described in this article is convenient to perform and reproducible and was a useful tool for detecting toxins of microbial origin toward eukaryotic cells not detectable in building materials by the other methods.
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- Dombrink-Kurtzman M. A., Bennett G. A., Richard J. L. An optimized MTT bioassay for determination of cytotoxicity of fumonisins in turkey lymphocytes. J AOAC Int. 1994 Mar-Apr;77(2):512–516. [PubMed] [Google Scholar]
- Fogelmark B., Sjöstrand M., Rylander R. Pulmonary inflammation induced by repeated inhalations of beta(1,3)-D-glucan and endotoxin. Int J Exp Pathol. 1994 Apr;75(2):85–90. [PMC free article] [PubMed] [Google Scholar]
- Hendry K. M., Cole E. C. A review of mycotoxins in indoor air. J Toxicol Environ Health. 1993 Feb;38(2):183–198. doi: 10.1080/15287399309531711. [DOI] [PubMed] [Google Scholar]
- Januskauskas A., Rodriguez-Martinez H. Assessment of sperm viability by measurement of ATP, membrane integrity and motility in frozen/thawed bull semen. Acta Vet Scand. 1995;36(4):571–574. doi: 10.1186/BF03547671. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johanning E., Biagini R., Hull D., Morey P., Jarvis B., Landsbergis P. Health and immunology study following exposure to toxigenic fungi (Stachybotrys chartarum) in a water-damaged office environment. Int Arch Occup Environ Health. 1996;68(4):207–218. doi: 10.1007/BF00381430. [DOI] [PubMed] [Google Scholar]
- Johnson A. G. Molecular adjuvants and immunomodulators: new approaches to immunization. Clin Microbiol Rev. 1994 Jul;7(3):277–289. doi: 10.1128/cmr.7.3.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nikulin M., Pasanen A. L., Berg S., Hintikka E. L. Stachybotrys atra Growth and Toxin Production in Some Building Materials and Fodder under Different Relative Humidities. Appl Environ Microbiol. 1994 Sep;60(9):3421–3424. doi: 10.1128/aem.60.9.3421-3424.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rylander R. Endotoxins in the environment. Prog Clin Biol Res. 1995;392:79–90. [PubMed] [Google Scholar]
- Sakai K., Watanabe K., Masuda K., Tsuji M., Hasumi K., Endo A. Isolation, characterization and biological activities of novel triprenyl phenols as pancreatic cholesterol esterase inhibitors produced by Stachybotrys sp. F-1839. J Antibiot (Tokyo) 1995 Jun;48(6):447–456. doi: 10.7164/antibiotics.48.447. [DOI] [PubMed] [Google Scholar]
- Sorenson W. G., Frazer D. G., Jarvis B. B., Simpson J., Robinson V. A. Trichothecene mycotoxins in aerosolized conidia of Stachybotrys atra. Appl Environ Microbiol. 1987 Jun;53(6):1370–1375. doi: 10.1128/aem.53.6.1370-1375.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tai J. H., Pestka J. J. Synergistic interaction between the trichothecene T-2 toxin and Salmonella typhimurium lipopolysaccharide in C3H/HeN and C3H/HeJ mice. Toxicol Lett. 1988 Nov;44(1-2):191–200. doi: 10.1016/0378-4274(88)90146-4. [DOI] [PubMed] [Google Scholar]
- Taylor M. J., Lafarge-Frayssinet C., Luster M. I., Frayssinet C. Increased endotoxin sensitivity following T-2 toxin treatment is associated with increased absorption of endotoxin. Toxicol Appl Pharmacol. 1991 Jun 1;109(1):51–59. doi: 10.1016/0041-008x(91)90190-p. [DOI] [PubMed] [Google Scholar]