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. 1997 Feb;63(2):543–546. doi: 10.1128/aem.63.2.543-546.1997

Listeria monocytogenes Scott A transports glucose by high-affinity and low-affinity glucose transport systems.

C Parker 1, R W Hutkins 1
PMCID: PMC168347  PMID: 9023935

Abstract

Listeria monocytogenes transported glucose by a high-affinity phosphoenolpyruvate-dependent phosphotransferase system and a low-affinity proton motive force-mediated system. The low-affinity system (Km = 2.9 mM) was inhibited by 2-deoxyglucose and 6-deoxyglucose, whereas the high-affinity system (Km = 0.11 mM) was inhibited by 2-deoxyglucose and mannose but not 6-deoxyglucose. Cells and vesicles artificially energized with valinomycin transported glucose or 2-deoxyglucose at rates greater than those of de-energized cells, indicating that a membrane potential could drive uptake by the low-affinity system.

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Selected References

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