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. 1997 Feb;63(2):636–643. doi: 10.1128/aem.63.2.636-643.1997

Aerobic and anaerobic metabolism of 6,10,14-trimethylpentadecan-2-one by a denitrifying bacterium isolated from marine sediments.

J F Rontani 1, M J Gilewicz 1, V D Michotey 1, T L Zheng 1, P C Bonin 1, J C Bertrand 1
PMCID: PMC168353  PMID: 9023941

Abstract

This report describes the metabolism of 6,10,14-trimethylpentadecan-2-one by a denitrifying bacterium (Marinobacter sp. strain CAB) isolated from marine sediments. Under aerobic and denitrifying conditions, this strain efficiently degraded this ubiquitous isoprenoid ketone. Several bacterial metabolites, 4,8,12-trimethyl-tridecan-1-ol, 4,8,12-trimethyltridecanal, 4,8,12-trimethyltridecanoic acid, Z-3,7-dimethylocten-2-oic acid, Z-3,7,11-trimethyldodecen-2-oic acid, and 6,10,14-trimethylpentadecan-2-ol, were formally identified, and different pathways were proposed to explain the formation of such isoprenoid compounds.

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Selected References

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  1. Balderston W. L., Sherr B., Payne W. J. Blockage by acetylene of nitrous oxide reduction in Pseudomonas perfectomarinus. Appl Environ Microbiol. 1976 Apr;31(4):504–508. doi: 10.1128/aem.31.4.504-508.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bonin P., Gilewicz M., Bertrand J. C. Denitrification by a marine bacterium Pseudomonas nautica strain 617. Ann Inst Pasteur Microbiol. 1987 May-Jun;138(3):371–383. doi: 10.1016/0769-2609(87)90125-6. [DOI] [PubMed] [Google Scholar]
  3. Britton L. N., Brand J. M., Markovetz A. J. Source of oxygen in the conversion of 2-tridecanone to undecyl acetate by Pseudomonas cepacia and Nocardia sp. Biochim Biophys Acta. 1974 Oct 16;369(1):45–49. doi: 10.1016/0005-2760(74)90190-8. [DOI] [PubMed] [Google Scholar]
  4. Cantwell S. G., Lau E. P., Watt D. S., Fall R. R. Biodegradation of acyclic isoprenoids by Pseudomonas species. J Bacteriol. 1978 Aug;135(2):324–333. doi: 10.1128/jb.135.2.324-333.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chan Y. K., Knowles R. Measurement of denitrification in two freshwater sediments by an in situ acetylene inhibition method. Appl Environ Microbiol. 1979 Jun;37(6):1067–1072. doi: 10.1128/aem.37.6.1067-1072.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Donoghue N. A., Norris D. B., Trudgill P. W. The purification and properties of cyclohexanone oxygenase from Nocardia globerula CL1 and Acinetobacter NCIB 9871. Eur J Biochem. 1976 Mar 16;63(1):175–192. doi: 10.1111/j.1432-1033.1976.tb10220.x. [DOI] [PubMed] [Google Scholar]
  7. Fall R. R., Brown J. L., Schaeffer T. L. Enzyme recruitment allows the biodegradation of recalcitrant branched hydrocarbons by Pseudomonas citronellolis. Appl Environ Microbiol. 1979 Oct;38(4):715–722. doi: 10.1128/aem.38.4.715-722.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Faulkner D. V., Jurka J. Multiple aligned sequence editor (MASE). Trends Biochem Sci. 1988 Aug;13(8):321–322. doi: 10.1016/0968-0004(88)90129-6. [DOI] [PubMed] [Google Scholar]
  9. Gauthier M. J., Lafay B., Christen R., Fernandez L., Acquaviva M., Bonin P., Bertrand J. C. Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol. 1992 Oct;42(4):568–576. doi: 10.1099/00207713-42-4-568. [DOI] [PubMed] [Google Scholar]
  10. Gellerman J. L., Anderson W. H., Schlenk H. Synthesis and analysis of phytyl and phytenoyl wax esters. Lipids. 1975 Nov;10(11):656–661. doi: 10.1007/BF02532757. [DOI] [PubMed] [Google Scholar]
  11. Gillan F. T., Nichols P. D., Johns R. B., Bavor H. J. Phytol degradation by marine bacteria. Appl Environ Microbiol. 1983 May;45(5):1423–1428. doi: 10.1128/aem.45.5.1423-1428.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Klug M. J., Markovetz A. J. Utilization of aliphatic hydrocarbons by micro-organisms. Adv Microb Physiol. 1971;5:1–43. doi: 10.1016/s0065-2911(08)60404-x. [DOI] [PubMed] [Google Scholar]
  13. LUKINS H. B., FOSTER J. W. METHYL KETONE METABOLISM IN HYDROCARBON-UTILIZING MYCOBACTERIA. J Bacteriol. 1963 May;85:1074–1087. doi: 10.1128/jb.85.5.1074-1087.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Patrick M. A., Dugan P. R. Influence of hydrocarbons and derivatives on the polar lipid fatty acids of an Acinetobacter isolate. J Bacteriol. 1974 Jul;119(1):76–81. doi: 10.1128/jb.119.1.76-81.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Platen H., Schink B. Anaerobic degradation of acetone and higher ketones via carboxylation by newly isolated denitrifying bacteria. J Gen Microbiol. 1989 Apr;135(4):883–891. doi: 10.1099/00221287-135-4-883. [DOI] [PubMed] [Google Scholar]
  16. SEUBERT W. Degradation of isoprenoid compounds by micro-organisms. I. Isolation and characterization of an isoprenoid-degrading bacterium, Pseudomonas citronellolis n. sp. J Bacteriol. 1960 Mar;79:426–434. doi: 10.1128/jb.79.3.426-434.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987 Jul;4(4):406–425. doi: 10.1093/oxfordjournals.molbev.a040454. [DOI] [PubMed] [Google Scholar]
  18. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol. 1991 Jan;173(2):697–703. doi: 10.1128/jb.173.2.697-703.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

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