Skip to main content
NCBI home page
American Journal of Human Genetics logoLink to American Journal of Human Genetics
. 1990 Apr;46(4):720–728.

Physical and genetic mapping of polymorphic loci in Xq28 (DXS15, DXS52, and DXS134): analysis of a cosmid clone and a yeast artificial chromosome.

R Feil 1, G Palmieri 1, M d'Urso 1, R Heilig 1, I Oberlé 1, J L Mandel 1
PMCID: PMC1683651  PMID: 1969226

Abstract

Sequences corresponding to the Xq28 loci DXS15, DXS52, DXS134, and DXS130 were shown to be present in a 140-kb yeast artificial chromosome (YAC XY58, isolated by Little et al.). This YAC clone appears to contain a faithful copy of this genomic region, as shown by comparison with human DNA and with a cosmid clone that contains probes St14c (part of the DXS52 sequences) and cpX67 (DXS134). cpX67 and St14c are contained in 11 kb and detect the same MspI RFLP polymorphism. A comparison of the YAC restriction map and pulsed-field gel electrophoresis data leads us to propose the following order of loci: DXS52(VNTR)-DXS33-DXF22S3-DXS130-DXS134 -DXS52-DXS15-DXS52, this whole cluster being comprised within 575 kb. The physical proximity of the DXS15, DXS52, and DXS134 loci led us to reinvestigate recombination events that had been reported between these loci in families from the Centre d'Etude du Polymorphisme Humain. Our results do not support the assumption that this region shows increased recombination.

Full text

PDF
720

Images in this article

722Figure 1
on p.722
723Figure 2
on p.723
725Figure 4
on p.725

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arveiler B., Vincent A., Mandel J. L. Toward a physical map of the Xq28 region in man: linking color vision, G6PD, and coagulation factor VIII genes to an X-Y homology region. Genomics. 1989 May;4(4):460–471. doi: 10.1016/0888-7543(89)90269-3. [DOI] [PubMed] [Google Scholar]
  2. Bardoni B., Guioli S., Raimondi E., Heilig R., Mandel J. L., Ottolenghi S., Camerino G. Isolation and characterization of a family of sequences dispersed on the human X chromosome. Genomics. 1988 Jul;3(1):32–38. doi: 10.1016/0888-7543(88)90155-3. [DOI] [PubMed] [Google Scholar]
  3. Bell M. V., Patterson M. N., Dorkins H. R., Davies K. E. Physical mapping of DXS134 close to the DXS52 locus. Hum Genet. 1989 Apr;82(1):27–30. doi: 10.1007/BF00288266. [DOI] [PubMed] [Google Scholar]
  4. Brown W. T., Gross A., Chan C., Jenkins E. C., Mandel J. L., Oberlé I., Arveiler B., Novelli G., Thibodeau S., Hagerman R. Multilocus analysis of the fragile X syndrome. Hum Genet. 1988 Mar;78(3):201–205. doi: 10.1007/BF00291662. [DOI] [PubMed] [Google Scholar]
  5. Burke D. T., Carle G. F., Olson M. V. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science. 1987 May 15;236(4803):806–812. doi: 10.1126/science.3033825. [DOI] [PubMed] [Google Scholar]
  6. Davies K. E., Young B. D., Elles R. G., Hill M. E., Williamson R. Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry. Nature. 1981 Oct 1;293(5831):374–376. doi: 10.1038/293374a0. [DOI] [PubMed] [Google Scholar]
  7. Donis-Keller H., Green P., Helms C., Cartinhour S., Weiffenbach B., Stephens K., Keith T. P., Bowden D. W., Smith D. R., Lander E. S. A genetic linkage map of the human genome. Cell. 1987 Oct 23;51(2):319–337. doi: 10.1016/0092-8674(87)90158-9. [DOI] [PubMed] [Google Scholar]
  8. Drayna D., Davies K., Hartley D., Mandel J. L., Camerino G., Williamson R., White R. Genetic mapping of the human X chromosome by using restriction fragment length polymorphisms. Proc Natl Acad Sci U S A. 1984 May;81(9):2836–2839. doi: 10.1073/pnas.81.9.2836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Drayna D., White R. The genetic linkage map of the human X chromosome. Science. 1985 Nov 15;230(4727):753–758. doi: 10.1126/science.4059909. [DOI] [PubMed] [Google Scholar]
  10. Feinberg A. P., Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. doi: 10.1016/0003-2697(84)90381-6. [DOI] [PubMed] [Google Scholar]
  11. Gardiner K., Watkins P., Münke M., Drabkin H., Jones C., Patterson D. Partial physical map of human chromosome 21. Somat Cell Mol Genet. 1988 Nov;14(6):623–637. doi: 10.1007/BF01535316. [DOI] [PubMed] [Google Scholar]
  12. Heilig R., Lemaire C., Mandel J. L. A 230kb cosmid walk in the Duchenne muscular dystrophy gene: detection of a conserved sequence and of a possible deletion prone region. Nucleic Acids Res. 1987 Nov 25;15(22):9129–9142. doi: 10.1093/nar/15.22.9129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hofker M. H., Bergen A. A., Skraastad M. I., Carpenter N. J., Veenema H., Connor J. M., Bakker E., van Ommen G. J., Pearson P. L. Efficient isolation of X chromosome-specific single-copy probes from a cosmid library of a human X/hamster hybrid-cell line: mapping of new probes close to the locus for X-linked mental retardation. Am J Hum Genet. 1987 Apr;40(4):312–328. [PMC free article] [PubMed] [Google Scholar]
  14. Holm C., Meeks-Wagner D. W., Fangman W. L., Botstein D. A rapid, efficient method for isolating DNA from yeast. Gene. 1986;42(2):169–173. doi: 10.1016/0378-1119(86)90293-3. [DOI] [PubMed] [Google Scholar]
  15. Kretz P. L., Reid C. H., Greener A., Short J. M. Effect of lambda packaging extract mcr restriction activity on DNA cloning. Nucleic Acids Res. 1989 Jul 11;17(13):5409–5409. doi: 10.1093/nar/17.13.5409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lindsay S., Bird A. P. Use of restriction enzymes to detect potential gene sequences in mammalian DNA. 1987 May 28-Jun 3Nature. 327(6120):336–338. doi: 10.1038/327336a0. [DOI] [PubMed] [Google Scholar]
  17. Little R. D., Porta G., Carle G. F., Schlessinger D., D'Urso M. Yeast artificial chromosomes with 200- to 800-kilobase inserts of human DNA containing HLA, V kappa, 5S, and Xq24-Xq28 sequences. Proc Natl Acad Sci U S A. 1989 Mar;86(5):1598–1602. doi: 10.1073/pnas.86.5.1598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mandel J. L., Arveiler B., Camerino G., Hanauer A., Heilig R., Koenig M., Oberlé I. Genetic mapping of the human X chromosome: linkage analysis of the q26-q28 region that includes the fragile X locus and isolation of expressed sequences. Cold Spring Harb Symp Quant Biol. 1986;51(Pt 1):195–203. doi: 10.1101/sqb.1986.051.01.024. [DOI] [PubMed] [Google Scholar]
  19. Mandel J. L., Willard H. F., Nussbaum R. L., Romeo G., Puck J. M., Davies K. E. Report of the committee on the genetic constitution of the X chromosome. Cytogenet Cell Genet. 1989;51(1-4):384–437. doi: 10.1159/000132801. [DOI] [PubMed] [Google Scholar]
  20. Oberlé I., Camerino G., Kloepfer C., Moisan J. P., Grzeschik K. H., Hellkuhl B., Hors-Cayla M. C., Van Cong N., Weil D., Mandel J. L. Characterization of a set of X-linked sequences and of a panel of somatic cell hybrids useful for the regional mapping of the human X chromosome. Hum Genet. 1986 Jan;72(1):43–49. doi: 10.1007/BF00278816. [DOI] [PubMed] [Google Scholar]
  21. Oberlé I., Drayna D., Camerino G., White R., Mandel J. L. The telomeric region of the human X chromosome long arm: presence of a highly polymorphic DNA marker and analysis of recombination frequency. Proc Natl Acad Sci U S A. 1985 May;82(9):2824–2828. doi: 10.1073/pnas.82.9.2824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Oberlé I., Heilig R., Moisan J. P., Kloepfer C., Mattéi G. M., Mattéi J. F., Boué J., Froster-Iskenius U., Jacobs P. A., Lathrop G. M. Genetic analysis of the fragile-X mental retardation syndrome with two flanking polymorphic DNA markers. Proc Natl Acad Sci U S A. 1986 Feb;83(4):1016–1020. doi: 10.1073/pnas.83.4.1016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Patterson M., Bell M., Schwartz C., Davies K. Pulsed-field gel mapping studies in the vicinity of the fragile site at Xq27.3. Am J Med Genet. 1988 May-Jun;30(1-2):581–591. doi: 10.1002/ajmg.1320300159. [DOI] [PubMed] [Google Scholar]
  24. Patterson M., Kenwrick S., Thibodeau S., Faulk K., Mattei M. G., Mattei J. F., Davies K. E. Mapping of DNA markers close to the fragile site on the human X chromosome at Xq27.3. Nucleic Acids Res. 1987 Mar 25;15(6):2639–2651. doi: 10.1093/nar/15.6.2639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Veenema H., Carpenter N. J., Bakker E., Hofker M. H., Ward A. M., Pearson P. L. The fragile X syndrome in a large family. III. Investigations on linkage of flanking DNA markers with the fragile site Xq27. J Med Genet. 1987 Jul;24(7):413–421. doi: 10.1136/jmg.24.7.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wada M., Little R. D., Abidi F., Porta G., Labella T., Cooper T., Della Valle G., D'Urso M., Schlessinger D. Human Xq24-Xq28: approaches to mapping with yeast artificial chromosomes. Am J Hum Genet. 1990 Jan;46(1):95–106. [PMC free article] [PubMed] [Google Scholar]

Articles from American Journal of Human Genetics are provided here courtesy of American Society of Human Genetics

RESOURCES