Abstract
The complete nucleotide sequences of two alleles of cellobiose dehydrogenase, cdh-1 (3,627 bp) and cdh-2 (3,623 bp), from Phanerochaete chrysosporium OGC101 are reported. The nucleotide sequences of cdh-1 and cdh-2 exhibit 97% similarity. A total of eighty-six point mutations between cdh-1 and cdh-2 are observed. Both alleles have 14 exons, and the introns are located at exactly the same positions. The translation products of these alleles have identical amino acid sequences. Restriction fragment length polymorphism analyses of homokaryotic derivatives show segregation of the CDH alleles.
Full Text
The Full Text of this article is available as a PDF (400.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alic M., Letzring C., Gold M. H. Mating System and Basidiospore Formation in the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium. Appl Environ Microbiol. 1987 Jul;53(7):1464–1469. doi: 10.1128/aem.53.7.1464-1469.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ayers A. R., Ayers S. B., Eriksson K. E. Cellobiose oxidase, purification and partial characterization of a hemoprotein from Sporotrichum pulverulentum. Eur J Biochem. 1978 Sep 15;90(1):171–181. doi: 10.1111/j.1432-1033.1978.tb12588.x. [DOI] [PubMed] [Google Scholar]
- Bao W., Renganathan V. Cellobiose oxidase of Phanerochaete chrysosporium enhances crystalline cellulose degradation by cellulases. FEBS Lett. 1992 May 4;302(1):77–80. doi: 10.1016/0014-5793(92)80289-s. [DOI] [PubMed] [Google Scholar]
- Bao W., Usha S. N., Renganathan V. Purification and characterization of cellobiose dehydrogenase, a novel extracellular hemoflavoenzyme from the white-rot fungus Phanerochaete chrysosporium. Arch Biochem Biophys. 1993 Feb 1;300(2):705–713. doi: 10.1006/abbi.1993.1098. [DOI] [PubMed] [Google Scholar]
- Breathnach R., Benoist C., O'Hare K., Gannon F., Chambon P. Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4853–4857. doi: 10.1073/pnas.75.10.4853. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Canevascini G., Borer P., Dreyer J. L. Cellobiose dehydrogenases of Sporotrichum (Chrysosporium) thermophile. Eur J Biochem. 1991 May 23;198(1):43–52. doi: 10.1111/j.1432-1033.1991.tb15984.x. [DOI] [PubMed] [Google Scholar]
- Covert S. F., Bolduc J., Cullen D. Genomic organization of a cellulase gene family in Phanerochaete chrysosporium. Curr Genet. 1992 Nov;22(5):407–413. doi: 10.1007/BF00352442. [DOI] [PubMed] [Google Scholar]
- Covert S. F., Vanden Wymelenberg A., Cullen D. Structure, organization, and transcription of a cellobiohydrolase gene cluster from Phanerochaete chrysosporium. Appl Environ Microbiol. 1992 Jul;58(7):2168–2175. doi: 10.1128/aem.58.7.2168-2175.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gaskell J., Dieperink E., Cullen D. Genomic organization of lignin peroxidase genes of Phanerochaete chrysosporium. Nucleic Acids Res. 1991 Feb 11;19(3):599–603. doi: 10.1093/nar/19.3.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gold M. H., Alic M. Molecular biology of the lignin-degrading basidiomycete Phanerochaete chrysosporium. Microbiol Rev. 1993 Sep;57(3):605–622. doi: 10.1128/mr.57.3.605-622.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henriksson G., Pettersson G., Johansson G., Ruiz A., Uzcategui E. Cellobiose oxidase from Phanerochaete chrysosporium can be cleaved by papain into two domains. Eur J Biochem. 1991 Feb 26;196(1):101–106. doi: 10.1111/j.1432-1033.1991.tb15791.x. [DOI] [PubMed] [Google Scholar]
- Kersten P. J., Witek C., vanden Wymelenberg A., Cullen D. Phanerochaete chrysosporium glyoxal oxidase is encoded by two allelic variants: structure, genomic organization, and heterologous expression of glx1 and glx2. J Bacteriol. 1995 Nov;177(21):6106–6110. doi: 10.1128/jb.177.21.6106-6110.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Li B., Nagalla S. R., Renganathan V. Cloning of a cDNA encoding cellobiose dehydrogenase, a hemoflavoenzyme from Phanerochaete chrysosporium. Appl Environ Microbiol. 1996 Apr;62(4):1329–1335. doi: 10.1128/aem.62.4.1329-1335.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lymar E. S., Li B., Renganathan V. Purification and Characterization of a Cellulose-Binding (beta)-Glucosidase from Cellulose-Degrading Cultures of Phanerochaete chrysosporium. Appl Environ Microbiol. 1995 Aug;61(8):2976–2980. doi: 10.1128/aem.61.8.2976-2980.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raices M., Paifer E., Cremata J., Montesino R., Ståhlberg J., Divne C., Szabó I. J., Henriksson G., Johansson G., Pettersson G. Cloning and characterization of a cDNA encoding a cellobiose dehydrogenase from the white rot fungus Phanerochaete chrysosporium. FEBS Lett. 1995 Aug 7;369(2-3):233–238. doi: 10.1016/0014-5793(95)00758-2. [DOI] [PubMed] [Google Scholar]
- Roy B. P., Archibald F. An indirect free radical-based assay for the enzyme cellobiose:quinone oxidoreductase. Anal Biochem. 1994 Feb 1;216(2):291–298. doi: 10.1006/abio.1994.1044. [DOI] [PubMed] [Google Scholar]
- Schmidhalter D. R., Canevascini G. Isolation and characterization of the cellobiose dehydrogenase from the brown-rot fungus Coniophora puteana (Schum ex Fr.) Karst. Arch Biochem Biophys. 1993 Feb 1;300(2):559–563. doi: 10.1006/abbi.1993.1077. [DOI] [PubMed] [Google Scholar]
- Sims P. F., Soares-Felipe M. S., Wang Q., Gent M. E., Tempelaars C., Broda P. Differential expression of multiple exo-cellobiohydrolase I-like genes in the lignin-degrading fungus Phanerochaete chrysosporium. Mol Microbiol. 1994 Apr;12(2):209–216. doi: 10.1111/j.1365-2958.1994.tb01010.x. [DOI] [PubMed] [Google Scholar]
- Strauss E. C., Kobori J. A., Siu G., Hood L. E. Specific-primer-directed DNA sequencing. Anal Biochem. 1986 Apr;154(1):353–360. doi: 10.1016/0003-2697(86)90536-1. [DOI] [PubMed] [Google Scholar]