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. 1997 Feb;63(2):803–805. doi: 10.1128/aem.63.2.803-805.1997

Novel group within the kingdom Crenarchaeota from boreal forest soil.

G Jurgens 1, K Lindström 1, A Saano 1
PMCID: PMC168374  PMID: 9023962

Abstract

We report here the results of phylogenetic analysis of archaeal 16S rRNA gene sequences amplified by PCR with Archaea-specific primers with mixed-population DNA extracted directly from forest soil used as a template. Nucleotide signature and phylogenetic analyses show that the sequences obtained belong to the domain Archaea and form a new cluster. Its phylogenetic position suggests that sequences are from a previously undescribed terrestrial group within the kingdom Crenarchaeota.

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Selected References

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  1. Amann R. I., Ludwig W., Schleifer K. H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev. 1995 Mar;59(1):143–169. doi: 10.1128/mr.59.1.143-169.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barns S. M., Delwiche C. F., Palmer J. D., Pace N. R. Perspectives on archaeal diversity, thermophily and monophyly from environmental rRNA sequences. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9188–9193. doi: 10.1073/pnas.93.17.9188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barns S. M., Fundyga R. E., Jeffries M. W., Pace N. R. Remarkable archaeal diversity detected in a Yellowstone National Park hot spring environment. Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1609–1613. doi: 10.1073/pnas.91.5.1609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Borneman J., Skroch P. W., O'Sullivan K. M., Palus J. A., Rumjanek N. G., Jansen J. L., Nienhuis J., Triplett E. W. Molecular microbial diversity of an agricultural soil in Wisconsin. Appl Environ Microbiol. 1996 Jun;62(6):1935–1943. doi: 10.1128/aem.62.6.1935-1943.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bruce K. D., Hiorns W. D., Hobman J. L., Osborn A. M., Strike P., Ritchie D. A. Amplification of DNA from native populations of soil bacteria by using the polymerase chain reaction. Appl Environ Microbiol. 1992 Oct;58(10):3413–3416. doi: 10.1128/aem.58.10.3413-3416.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DeLong E. F. Archaea in coastal marine environments. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5685–5689. doi: 10.1073/pnas.89.12.5685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fuhrman J. A., McCallum K., Davis A. A. Novel major archaebacterial group from marine plankton. Nature. 1992 Mar 12;356(6365):148–149. doi: 10.1038/356148a0. [DOI] [PubMed] [Google Scholar]
  8. Giovannoni S. J., DeLong E. F., Olsen G. J., Pace N. R. Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells. J Bacteriol. 1988 Feb;170(2):720–726. doi: 10.1128/jb.170.2.720-726.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hales B. A., Edwards C., Ritchie D. A., Hall G., Pickup R. W., Saunders J. R. Isolation and identification of methanogen-specific DNA from blanket bog peat by PCR amplification and sequence analysis. Appl Environ Microbiol. 1996 Feb;62(2):668–675. doi: 10.1128/aem.62.2.668-675.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Liesack W., Stackebrandt E. Occurrence of novel groups of the domain Bacteria as revealed by analysis of genetic material isolated from an Australian terrestrial environment. J Bacteriol. 1992 Aug;174(15):5072–5078. doi: 10.1128/jb.174.15.5072-5078.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maidak B. L., Larsen N., McCaughey M. J., Overbeek R., Olsen G. J., Fogel K., Blandy J., Woese C. R. The Ribosomal Database Project. Nucleic Acids Res. 1994 Sep;22(17):3485–3487. doi: 10.1093/nar/22.17.3485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McInerney J. O., Wilkinson M., Patching J. W., Embley T. M., Powell R. Recovery and phylogenetic analysis of novel archaeal rRNA sequences from a deep-sea deposit feeder. Appl Environ Microbiol. 1995 Apr;61(4):1646–1648. doi: 10.1128/aem.61.4.1646-1648.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Winker S., Woese C. R. A definition of the domains Archaea, Bacteria and Eucarya in terms of small subunit ribosomal RNA characteristics. Syst Appl Microbiol. 1991;14(4):305–310. doi: 10.1016/S0723-2020(11)80303-6. [DOI] [PubMed] [Google Scholar]
  14. Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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