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. 1997 Feb;63(2):812–814. doi: 10.1128/aem.63.2.812-814.1997

Alkaline stress response in Enterococcus faecalis: adaptation, cross-protection, and changes in protein synthesis.

S Flahaut 1, A Hartke 1, J C Giard 1, Y Auffray 1
PMCID: PMC168376  PMID: 9023964

Abstract

The alkaline shock response in Enterococcus faecalis was studied in this work. Cells adapted to an optimum pH of 10.5 were tolerate to pH 11.9 conditions but acquired sensitivity to acid damage. An analysis of stress proteins revealed that 37 polypeptides were amplified. Two of these are DnaK and GroEL. The combined results show that bile salts and alkaline stress responses are closely related.

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Selected References

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  1. Bingham R. J., Hall K. S., Slonczewski J. L. Alkaline induction of a novel gene locus, alx, in Escherichia coli. J Bacteriol. 1990 Apr;172(4):2184–2186. doi: 10.1128/jb.172.4.2184-2186.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Flahaut S., Frere J., Boutibonnes P., Auffray Y. Comparison of the bile salts and sodium dodecyl sulfate stress responses in Enterococcus faecalis. Appl Environ Microbiol. 1996 Jul;62(7):2416–2420. doi: 10.1128/aem.62.7.2416-2420.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Flahaut S., Hartke A., Giard J. C., Benachour A., Boutibonnes P., Auffray Y. Relationship between stress response toward bile salts, acid and heat treatment in Enterococcus faecalis. FEMS Microbiol Lett. 1996 Apr 15;138(1):49–54. doi: 10.1111/j.1574-6968.1996.tb08133.x. [DOI] [PubMed] [Google Scholar]
  4. Foster J. W., Aliabadi Z. pH-regulated gene expression in Salmonella: genetic analysis of aniG and cloning of the earA regulator. Mol Microbiol. 1989 Nov;3(11):1605–1615. doi: 10.1111/j.1365-2958.1989.tb00146.x. [DOI] [PubMed] [Google Scholar]
  5. Hartke A, Bouche S, Giard JC, Benachour A, Boutibonnes P, Auffray Y. The Lactic Acid Stress Response of Lactococcus lactis subsp. lactis. Curr Microbiol. 1996 Sep;33(3):194–199. doi: 10.1007/s002849900099. [DOI] [PubMed] [Google Scholar]
  6. Kakinuma Y., Igarashi K. Amplification of the Na+-ATPase of Streptococcus faecalis at alkaline pH. FEBS Lett. 1990 Feb 12;261(1):135–138. doi: 10.1016/0014-5793(90)80654-2. [DOI] [PubMed] [Google Scholar]
  7. Karem K. L., Foster J. W., Bej A. K. Adaptive acid tolerance response (ATR) in Aeromonas hydrophila. Microbiology. 1994 Jul;140(Pt 7):1731–1736. doi: 10.1099/13500872-140-7-1731. [DOI] [PubMed] [Google Scholar]
  8. Kuroda M., Ohta T., Hayashi H. Isolation and the gene cloning of an alkaline shock protein in methicillin resistant Staphylococcus aureus. Biochem Biophys Res Commun. 1995 Feb 27;207(3):978–984. doi: 10.1006/bbrc.1995.1281. [DOI] [PubMed] [Google Scholar]
  9. Mugikura S., Nishikawa M., Igarashi K., Kobayashi H. Maintenance of a neutral cytoplasmic pH is not obligatory for growth of Escherichia coli and Streptococcus faecalis at an alkaline pH. J Biochem. 1990 Jul;108(1):86–91. doi: 10.1093/oxfordjournals.jbchem.a123168. [DOI] [PubMed] [Google Scholar]
  10. Padan E., Schuldiner S. Intracellular pH and membrane potential as regulators in the prokaryotic cell. J Membr Biol. 1987;95(3):189–198. doi: 10.1007/BF01869481. [DOI] [PubMed] [Google Scholar]
  11. Raja N., Goodson M., Chui W. C., Smith D. G., Rowbury R. J. Habituation to acid in Escherichia coli: conditions for habituation and its effects on plasmid transfer. J Appl Bacteriol. 1991 Jan;70(1):59–65. doi: 10.1111/j.1365-2672.1991.tb03787.x. [DOI] [PubMed] [Google Scholar]
  12. Rowbury R. J., Hussain N. H. Exposure of Escherichia coli to acid habituation conditions sensitizes it to alkaline stress. Lett Appl Microbiol. 1996 Jan;22(1):57–61. doi: 10.1111/j.1472-765x.1996.tb01108.x. [DOI] [PubMed] [Google Scholar]
  13. Rowbury R. J., Lazim Z., Goodson M. Regulatory aspects of alkali tolerance induction in Escherichia coli. Lett Appl Microbiol. 1996 Jun;22(6):429–432. doi: 10.1111/j.1472-765x.1996.tb01196.x. [DOI] [PubMed] [Google Scholar]
  14. Schuldiner S., Agmon V., Brandsma J., Cohen A., Friedman E., Padan E. Induction of SOS functions by alkaline intracellular pH in Escherichia coli. J Bacteriol. 1986 Nov;168(2):936–939. doi: 10.1128/jb.168.2.936-939.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Taglicht D., Padan E., Oppenheim A. B., Schuldiner S. An alkaline shift induces the heat shock response in Escherichia coli. J Bacteriol. 1987 Feb;169(2):885–887. doi: 10.1128/jb.169.2.885-887.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Zilberstein D., Agmon V., Schuldiner S., Padan E. Escherichia coli intracellular pH, membrane potential, and cell growth. J Bacteriol. 1984 Apr;158(1):246–252. doi: 10.1128/jb.158.1.246-252.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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