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. 1990 Jun;46(6):1149–1155.

Theoretical underpinning of the single-molecule-dilution (SMD) method of direct haplotype resolution.

J C Stephens 1, J Rogers 1, G Ruano 1
PMCID: PMC1683843  PMID: 2339707

Abstract

In a recent paper we have shown that DNA haplotypes of multiply heterozygous individuals can be resolved directly by polymerase-chain-reaction (PCR) amplification of a single molecule of genomic template. Our method (the single-molecule-dilution [SMD] method) relies on the stochastic separation of maternal and paternal alleles at high dilution. The stochasticity of separation and the potential for DNA shearing (which could separate the loci of interest) are two factors that can compromise the results of the experiment. This paper explores the consequences of these two factors and shows that the SMD method can be expected to work very reliably even in the presence of a moderate amount of DNA shearing.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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