Skip to main content
PMC home page
American Journal of Human Genetics logoLink to American Journal of Human Genetics
. 1990 Dec;47(6):952–956.

A new DNA marker (D10S94) very tightly linked to the multiple endocrine neoplasia type 2A (MEN2A) locus.

P J Goodfellow 1, S Myers 1, L L Anderson 1, A R Brooks-Wilson 1, N E Simpson 1
PMCID: PMC1683904  PMID: 1978561

Abstract

Combined somatic cell hybrid and linkage studies between D10S94 and five pericentromeric loci (FNRB, D10Z1, MEN2A, RBP3, and D10S15) have localized the new DNA sequence pcl1/A1S-6-c23 at D10S94 to 10q11.2. No recombinants were observed between D10S94 and D10Z1 or MEN2A. D10S94 maps in proximal 10q11.2 very near to MEN2A. There are three possible orders for the six loci that we investigated from the centromeric region of chromosome 10. At present the genetic data do not allow us to order MEN2A with respect to D10Z1 and D10S94. The three possible orders are FNRB-D10Z1-D10S94-MEN2A-RBP3-D10S15, FNRB-D10Z1-MEN2A-D10S94-RBP3-D10S15, and FNRB-MEN2A-D10Z1-D10S94-RBP3-D10S15. In view of the fact that no recombinants between D10S94 and MEN2A or between D10S94 and D10Z1 were observed, the combined haplotypes formed from RFLPs and D10Z1 and D10S94 will increase the informativeness and accuracy of genotype prediction for at-risk members of the families having the MEN 2A syndrome, particularly when the affected parent is female. The localization of D10S94 with respect to MEN2A will prove valuable in experiments directed toward cloning the MEN2A locus.

Full text

PDF
952

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brooks-Wilson A. R., Goodfellow P. N., Povey S., Nevanlinna H. A., de Jong P. J., Goodfellow P. J. Rapid cloning and characterization of new chromosome 10 DNA markers by Alu element-mediated PCR. Genomics. 1990 Aug;7(4):614–620. doi: 10.1016/0888-7543(90)90207-b. [DOI] [PubMed] [Google Scholar]
  2. Carson N. L., Wu J. S., Jackson C. E., Kidd K. K., Simpson N. E. The mutation for medullary thyroid carcinoma with parathyroid tumors (MTC with PTs) is closely linked to the centromeric region of chromosome 10. Am J Hum Genet. 1990 Dec;47(6):946–951. [PMC free article] [PubMed] [Google Scholar]
  3. Devilee P., Kievits T., Waye J. S., Pearson P. L., Willard H. F. Chromosome-specific alpha satellite DNA: isolation and mapping of a polymorphic alphoid repeat from human chromosome 10. Genomics. 1988 Jul;3(1):1–7. doi: 10.1016/0888-7543(88)90151-6. [DOI] [PubMed] [Google Scholar]
  4. Easton D. F., Ponder M. A., Cummings T., Gagel R. F., Hansen H. H., Reichlin S., Tashjian A. H., Jr, Telenius-Berg M., Ponder B. A. The clinical and screening age-at-onset distribution for the MEN-2 syndrome. Am J Hum Genet. 1989 Feb;44(2):208–215. [PMC free article] [PubMed] [Google Scholar]
  5. Farrer L. A., Goodfellow P. J., White B. N., Holden J. J., Kidd J. R., Simpson N. E., Kidd K. K. Linkage analysis of multiple endocrine neoplasia type 2A (MEN-2A) and three DNA markers on chromosome 20: evidence against synteny. Cancer Genet Cytogenet. 1987 Aug;27(2):327–334. doi: 10.1016/0165-4608(87)90015-x. [DOI] [PubMed] [Google Scholar]
  6. Gagel R. F., Tashjian A. H., Jr, Cummings T., Papathanasopoulos N., Kaplan M. M., DeLellis R. A., Wolfe H. J., Reichlin S. The clinical outcome of prospective screening for multiple endocrine neoplasia type 2a. An 18-year experience. N Engl J Med. 1988 Feb 25;318(8):478–484. doi: 10.1056/NEJM198802253180804. [DOI] [PubMed] [Google Scholar]
  7. Goodfellow P. J., Nevanlinna H. A., Gorman P., Sheer D., Lam G., Goodfellow P. N. Assignment of the gene encoding the beta-subunit of the human fibronectin receptor (beta-FNR) to chromosome 10p11.2. Ann Hum Genet. 1989 Jan;53(Pt 1):15–22. doi: 10.1111/j.1469-1809.1989.tb01118.x. [DOI] [PubMed] [Google Scholar]
  8. Goodfellow P. J., Povey S., Nevanlinna H. A., Goodfellow P. N. Generation of a panel of somatic cell hybrids containing unselected fragments of human chromosome 10 by X-ray irradiation and cell fusion: application to isolating the MEN2A region in hybrid cells. Somat Cell Mol Genet. 1990 Mar;16(2):163–171. doi: 10.1007/BF01233046. [DOI] [PubMed] [Google Scholar]
  9. Lathrop M., Nakamura Y., Cartwright P., O'Connell P., Leppert M., Jones C., Tateishi H., Bragg T., Lalouel J. M., White R. A primary genetic map of markers of human chromosome 10. Genomics. 1988 Feb;2(2):157–164. doi: 10.1016/0888-7543(88)90098-5. [DOI] [PubMed] [Google Scholar]
  10. Liou G. I., Fong S. L., Gosden J., van Tuinen P., Ledbetter D. H., Christie S., Rout D., Bhattacharya S., Cook R. G., Li Y. Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization. Somat Cell Mol Genet. 1987 Jul;13(4):315–323. doi: 10.1007/BF01534925. [DOI] [PubMed] [Google Scholar]
  11. MORTON N. E. Sequential tests for the detection of linkage. Am J Hum Genet. 1955 Sep;7(3):277–318. [PMC free article] [PubMed] [Google Scholar]
  12. Mathew C. G., Chin K. S., Easton D. F., Thorpe K., Carter C., Liou G. I., Fong S. L., Bridges C. D., Haak H., Kruseman A. C. A linked genetic marker for multiple endocrine neoplasia type 2A on chromosome 10. Nature. 1987 Aug 6;328(6130):527–528. doi: 10.1038/328527a0. [DOI] [PubMed] [Google Scholar]
  13. Mathew C. G., Wakeling W., Jones E., Easton D., Fisher R., Strong C., Smith B., Chin K., Little P., Nakamura Y. Regional localization of polymorphic markers on chromosome 10 by physical and genetic mapping. Ann Hum Genet. 1990 May;54(Pt 2):121–129. doi: 10.1111/j.1469-1809.1990.tb00368.x. [DOI] [PubMed] [Google Scholar]
  14. Nakamura Y., Lathrop M., Bragg T., Leppert M., O'Connell P., Jones C., Lalouel J. M., White R. An extended genetic linkage map of markers for human chromosome 10. Genomics. 1988 Nov;3(4):389–392. doi: 10.1016/0888-7543(88)90133-4. [DOI] [PubMed] [Google Scholar]
  15. Nakamura Y., Mathew C. G., Sobol H., Easton D. F., Telenius H., Bragg T., Chin K., Clark J., Jones C., Lenoir G. M. Linked markers flanking the gene for multiple endocrine neoplasia type 2A. Genomics. 1989 Aug;5(2):199–203. doi: 10.1016/0888-7543(89)90046-3. [DOI] [PubMed] [Google Scholar]
  16. Narod S. A., Sobol H., Nakamura Y., Calmettes C., Baulieu J. L., Bigorgne J. C., Chabrier G., Couette J., de Gennes J. L., Duprey J. Linkage analysis of hereditary thyroid carcinoma with and without pheochromocytoma. Hum Genet. 1989 Nov;83(4):353–358. doi: 10.1007/BF00291380. [DOI] [PubMed] [Google Scholar]
  17. Ott J. Estimation of the recombination fraction in human pedigrees: efficient computation of the likelihood for human linkage studies. Am J Hum Genet. 1974 Sep;26(5):588–597. [PMC free article] [PubMed] [Google Scholar]
  18. Simpson N. E., Kidd K. K., Goodfellow P. J., McDermid H., Myers S., Kidd J. R., Jackson C. E., Duncan A. M., Farrer L. A., Brasch K. Assignment of multiple endocrine neoplasia type 2A to chromosome 10 by linkage. Nature. 1987 Aug 6;328(6130):528–530. doi: 10.1038/328528a0. [DOI] [PubMed] [Google Scholar]
  19. Smith M., Simpson N. E. Report of the committee on the genetic constitution of chromosomes 9 and 10. Cytogenet Cell Genet. 1989;51(1-4):202–225. doi: 10.1159/000132792. [DOI] [PubMed] [Google Scholar]
  20. Wu J. S., Carson N. L., Myers S., Pakstis A. J., Kidd J. R., Castiglione C. M., Anderson L., Hoyle L. S., Genel M., Verdy M. The genetic defect in multiple endocrine neoplasia type 2A maps next to the centromere of chromosome 10. Am J Hum Genet. 1990 Mar;46(3):624–630. [PMC free article] [PubMed] [Google Scholar]
  21. Wu J. S., Giuffra L. A., Goodfellow P. J., Myers S., Carson N. L., Anderson L., Hoyle L. S., Simpson N. E., Kidd K. K. The beta subunit locus of the human fibronectin receptor: DNA restriction fragment length polymorphism and linkage mapping studies. Hum Genet. 1989 Nov;83(4):383–390. doi: 10.1007/BF00291386. [DOI] [PubMed] [Google Scholar]

Articles from American Journal of Human Genetics are provided here courtesy of American Society of Human Genetics

RESOURCES