Skip to main content
American Journal of Human Genetics logoLink to American Journal of Human Genetics
. 1986 Aug;39(2):232–238.

Immunochemical studies on lactate dehydrogenase A subunit deficiencies.

M Maekawa, K Sudo, T Kanno
PMCID: PMC1683931  PMID: 3092644

Abstract

In lactate dehydrogenase (LDH) A subunit deficiency, is there not only a lack of activity but also a lack of subunit production? We demonstrated three remarkable points to answer this question: There are no proteins that immunologically react with anti-A subunits. There are no heterotetramers that react with anti-B subunits. B subunits seem to be genetically produced at normal level, and all of them form only one isoenzyme, LDH-B4. From these points, we concluded that there is a complete lack of A subunit production or production of incomplete A subunits that can neither react with anti-A subunits nor form heterotetramers.

Full text

PDF
232

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alper C. A., Johnson A. M. Immunofixation electrophoresis: a technique for the study of protein polymorphism. Vox Sang. 1969 Nov;17(5):445–452. doi: 10.1111/j.1423-0410.1969.tb00414.x. [DOI] [PubMed] [Google Scholar]
  2. Burd J. F., Usategui-Gomez M., Fernandez de Castro A., Mhatre N. S., Yeager F. M. Immunochemical studies on lactate dehydrogenase: preparation and characterization of anti-M lactate dehydrogenase antiserum: development of an immunochemical assay for lactate dehydrogenase isoenzymes in human serum. Clin Chim Acta. 1973 Jul 14;46(3):205–215. doi: 10.1016/0009-8981(73)90172-1. [DOI] [PubMed] [Google Scholar]
  3. Kanno T., Sudo K., Takeuchi I., Kanda S., Honda N., Nishimura Y., Oyama K. Hereditary deficiency of lactate dehydrogenase M-subunit. Clin Chim Acta. 1980 Dec 8;108(2):267–276. doi: 10.1016/0009-8981(80)90013-3. [DOI] [PubMed] [Google Scholar]
  4. Kitamura M., Iijima N., Hashimoto F., Hiratsuka A. Hereditary deficiency of subunit H of lactate dehydrogenase. Clin Chim Acta. 1971 Oct;34(3):419–423. doi: 10.1016/0009-8981(71)90095-7. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Maekawa M., Kanda S., Sudo K., Kanno T. Estimation of the gene frequency of lactate dehydrogenase subunit deficiencies. Am J Hum Genet. 1984 Nov;36(6):1204–1214. [PMC free article] [PubMed] [Google Scholar]
  7. Malvano R., Massaglia A., Zannino M., Palmucci F., Calì V., Zucchelli G. C. Radioimmunoassay of lactate dehydrogenase, H forms. Eur J Nucl Med. 1979 Oct;4(5):379–384. doi: 10.1007/BF00263308. [DOI] [PubMed] [Google Scholar]
  8. Markert C. L., Møller F. MULTIPLE FORMS OF ENZYMES: TISSUE, ONTOGENETIC, AND SPECIES SPECIFIC PATTERNS. Proc Natl Acad Sci U S A. 1959 May;45(5):753–763. doi: 10.1073/pnas.45.5.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  10. Shioya M., Yanagisawa M., Kawamura T., Kanno T. [Separation of LDH isoenzymes by cellulose acetate (Cellogel)]. Rinsho Byori. 1971 Jul;19(7):469–472. [PubMed] [Google Scholar]
  11. WROBLEWSKI F., LADUE J. S. Lactic dehydrogenase activity in blood. Proc Soc Exp Biol Med. 1955 Oct;90(1):210–213. doi: 10.3181/00379727-90-21985. [DOI] [PubMed] [Google Scholar]
  12. van KAMPEN E., ZIJLSTRA W. G. Standardization of hemoglobinometry. II. The hemiglobincyanide method. Clin Chim Acta. 1961 Jul;6:538–544. doi: 10.1016/0009-8981(61)90145-0. [DOI] [PubMed] [Google Scholar]

Articles from American Journal of Human Genetics are provided here courtesy of American Society of Human Genetics

RESOURCES