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. 1997 Mar;63(3):1143–1147. doi: 10.1128/aem.63.3.1143-1147.1997

Molecular beacons: trial of a fluorescence-based solution hybridization technique for ecological studies with ruminal bacteria.

P Schofield 1, A N Pell 1, D O Krause 1
PMCID: PMC168404  PMID: 9055429

Abstract

Molecular beacons are fluorescent probes developed for solution rather than membrane hybridization. We have investigated the utility of these probes to study rumen microbial ecology. Two cellulolytic species, Ruminococcus albus and Fibrobacter succinogenes, were tested. Membrane and solution hybridizations gave similar results in competition experiments with cocultures of R. albus 8 and F. succinogenes S85.

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Selected References

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