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. 1987 Feb;40(2):115–125.

cDNA cloning and mapping of the human creatine kinase M gene to 19q13

Janice M Nigro, Clifford W Schweinfest, Aleksandar Rajkovic, Jovan Pavlovic, Sumayah Jamal, Robert P Dottin, John T Hart, Michael E Kamarck, Peter M M Rae, Maynard D Carty, Patricia Martin-Deleon
PMCID: PMC1684079  PMID: 3031982

Abstract

We describe the first isolation of a human creatine kinase M cDNA clone and its mapping of the gene to human chromosome 19. A human creatine kinase M cDNA clone, pJN2CK-M, harboring a 1,160-bp insert, was isolated by colony hybridization with a previously se-quenced chicken creatine kinase M cDNA probe. The human cDNA was used as a probe in Southern transfers of TaqI-digested genomic DNA from mouse/human somatic-cell hybrids to localize the human creatine kinase-M gene to chromosome 19. In situ hybridization of the tritiated cDNA probe to metaphase chromosomes of peripheral blood lymphocytes from normal males revealed significant labeling to chromosome 19. These two independent methodologies assign the human creatine kinase-M gene to chromosome 19. Since >69% of the grains of chromosome 19 label band q13, the human creatine kinase-M gene has been mapped to 19ql3. On the basis of high-resolution G-banding, the predominant labeling site was 19q13.2-q13.3.

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Selected References

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