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. 1997 Mar;63(3):1185–1188. doi: 10.1128/aem.63.3.1185-1188.1997

Assessment of fecal bacteria with bile acid 7 alpha-dehydroxylating activity for the presence of bai-like genes.

K C Doerner 1, F Takamine 1, C P LaVoie 1, D H Mallonee 1, P B Hylemon 1
PMCID: PMC168411  PMID: 9055436

Abstract

Eubacterium sp. strain VPI 12708 has several bile acid-inducible (bai) genes which encode enzymes in the bile acid 7 alpha-dehydroxylation (7 alpha DeOH) pathway. Twelve 7 alpha DeOH-positive intestinal bacterial strains were assayed for 7 alpha DeOH activity, and 13 strains were tested for hybridization with bai genes. Cholic acid 7 alpha DeOH activity varied greatly (> 100-fold) among these strains. Southern blot experiments showed that DNA prepared from 7 of 13 strains hybridized with at least one of the bai genes from Eubacterium sp. strain VPI 12708.

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Selected References

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