Skip to main content
PMC home page
American Journal of Human Genetics logoLink to American Journal of Human Genetics
. 1987 Jun;40(6):503–511.

The coding sequence for the 32,000-dalton pulmonary surfactant-associated protein A is located on chromosome 10 and identifies two separate restriction-fragment-length polymorphisms.

J H Fisher, F T Kao, C Jones, R T White, B J Benson, R J Mason
PMCID: PMC1684155  PMID: 2884868

Abstract

The primary protein component of human pulmonary surfactant is a 32,000-dalton glycoprotein called surfactant-associated protein A. This protein is important for normal lung function, and its expression is developmentally regulated. Using a mapping panel of somatic-cell hybrids, we have localized the coding sequence for pulmonary surfactant-associated protein A to chromosome 10. Additionally, this sequence identifies two separate MspI restriction-fragment-length polymorphisms. Since there is a relative lack of polymorphic markers for chromosome 10, this sequence may be useful in linkage analysis.

Full text

PDF
503

Images in this article

506Fig. 1
on p.506
507Fig. 2
on p.507

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cheung P., Kao F. T., Law M. L., Jones C., Puck T. T., Chan L. Localization of the structural gene for human apolipoprotein A-I on the long arm of human chromosome 11. Proc Natl Acad Sci U S A. 1984 Jan;81(2):508–511. doi: 10.1073/pnas.81.2.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fisher J. H., Miller Y. E., Sparkes R. S., Bateman J. B., Kimmel K. A., Carey T. E., Rodell T., Shoemaker S. A., Scoggin C. H. Wilms' tumor-aniridia association: segregation of affected chromosome in somatic cell hybrids, identification of cell surface antigen associated with deleted area, and regional mapping of c-Ha-ras-1 oncogene, insulin gene, and beta-globin gene. Somat Cell Mol Genet. 1984 Sep;10(5):455–464. doi: 10.1007/BF01534850. [DOI] [PubMed] [Google Scholar]
  3. Floros J., Phelps D. S., Taeusch H. W. Biosynthesis and in vitro translation of the major surfactant-associated protein from human lung. J Biol Chem. 1985 Jan 10;260(1):495–500. [PubMed] [Google Scholar]
  4. Floros J., Steinbrink R., Jacobs K., Phelps D., Kriz R., Recny M., Sultzman L., Jones S., Taeusch H. W., Frank H. A. Isolation and characterization of cDNA clones for the 35-kDa pulmonary surfactant-associated protein. J Biol Chem. 1986 Jul 5;261(19):9029–9033. [PubMed] [Google Scholar]
  5. Gusella J., Varsanyi-Breiner A., Kao F. T., Jones C., Puck T. T., Keys C., Orkin S., Housman D. Precise localization of human beta-globin gene complex on chromosome 11. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5239–5242. doi: 10.1073/pnas.76.10.5239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hallman M., Spragg R., Harrell J. H., Moser K. M., Gluck L. Evidence of lung surfactant abnormality in respiratory failure. Study of bronchoalveolar lavage phospholipids, surface activity, phospholipase activity, and plasma myoinositol. J Clin Invest. 1982 Sep;70(3):673–683. doi: 10.1172/JCI110662. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hawgood S., Benson B. J., Hamilton R. L., Jr Effects of a surfactant-associated protein and calcium ions on the structure and surface activity of lung surfactant lipids. Biochemistry. 1985 Jan 1;24(1):184–190. doi: 10.1021/bi00322a026. [DOI] [PubMed] [Google Scholar]
  8. Inui K., Kao F. T., Fujibayashi S., Jones C., Morse H. G., Law M. L., Wenger D. A. The gene coding for a sphingolipid activator protein, SAP-1, is on human chromosome 10. Hum Genet. 1985;69(3):197–200. doi: 10.1007/BF00293023. [DOI] [PubMed] [Google Scholar]
  9. Jones C., Morse H. G., Kao F. T., Carbone A., Palmer E. Human T-cell receptor alpha-chain genes: location on chromosome 14. Science. 1985 Apr 5;228(4695):83–85. doi: 10.1126/science.3919444. [DOI] [PubMed] [Google Scholar]
  10. Jones C. Synteny between the pro+ marker and human glutamate oxaloacetate transaminase. Somatic Cell Genet. 1975 Oct;1(4):345–354. doi: 10.1007/BF01538666. [DOI] [PubMed] [Google Scholar]
  11. Kao F. T., Hartz J. A., Law M. L., Davidson J. N. Isolation and chromosomal localization of unique DNA sequences from a human genomic library. Proc Natl Acad Sci U S A. 1982 Feb;79(3):865–869. doi: 10.1073/pnas.79.3.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kao F. T., Morse H. G., Law M. L., Lidsky A., Chandra T., Woo S. L. Genetic mapping of the structural gene for antithrombin III to human chromosome 1. Hum Genet. 1984;67(1):34–36. doi: 10.1007/BF00270555. [DOI] [PubMed] [Google Scholar]
  13. Katyal S. L., Singh G. Analysis of pulmonary surfactant apoproteins by isoelectric focusing. Biochim Biophys Acta. 1984 Jul 26;794(3):411–418. doi: 10.1016/0005-2760(84)90007-9. [DOI] [PubMed] [Google Scholar]
  14. King R. J., Ruch J., Gikas E. G., Platzker A. C., Creasy R. K. Appearance of paoproteins of pulmonary surfactant in human amniotic fluid. J Appl Physiol. 1975 Nov;39(5):735–741. doi: 10.1152/jappl.1975.39.5.735. [DOI] [PubMed] [Google Scholar]
  15. Kunkel L. M., Smith K. D., Boyer S. H., Borgaonkar D. S., Wachtel S. S., Miller O. J., Breg W. R., Jones H. W., Jr, Rary J. M. Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1245–1249. doi: 10.1073/pnas.74.3.1245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lin-Lee Y. C., Kao F. T., Cheung P., Chan L. Apolipoprotein E gene mapping and expression: localization of the structural gene to human chromosome 19 and expression of ApoE mRNA in lipoprotein- and non-lipoprotein-producing tissues. Biochemistry. 1985 Jul 2;24(14):3751–3756. doi: 10.1021/bi00335a050. [DOI] [PubMed] [Google Scholar]
  17. Natt E., Kao F. T., Rettenmeier R., Scherer G. Assignment of the human tyrosine aminotransferase gene to chromosome 16. Hum Genet. 1986 Mar;72(3):225–228. doi: 10.1007/BF00291882. [DOI] [PubMed] [Google Scholar]
  18. Ng V. L., Herndon V. L., Mendelson C. R., Snyder J. M. Characterization of rabbit surfactant-associated proteins. Biochim Biophys Acta. 1983 Nov 29;754(2):218–226. doi: 10.1016/0005-2760(83)90164-9. [DOI] [PubMed] [Google Scholar]
  19. Phelps D. S., Floros J., Taeusch H. W., Jr Post-translational modification of the major human surfactant-associated proteins. Biochem J. 1986 Jul 15;237(2):373–377. doi: 10.1042/bj2370373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Phelps D. S., Taeusch H. W., Jr A comparison of the major surfactant-associated proteins in different species. Comp Biochem Physiol B. 1985;82(3):441–446. doi: 10.1016/0305-0491(85)90006-9. [DOI] [PubMed] [Google Scholar]
  21. Phelps D. S., Taeusch H. W., Jr, Benson B., Hawgood S. An electrophoretic and immunochemical characterization of human surfactant-associated proteins. Biochim Biophys Acta. 1984 Dec 7;791(2):226–238. doi: 10.1016/0167-4838(84)90013-x. [DOI] [PubMed] [Google Scholar]
  22. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  23. Sueishi K., Benson B. J. Isolation of a major apolipoprotein of canine and murine pulmonary surfactant. Biochemical and immunochemical characteristics. Biochim Biophys Acta. 1981 Sep 24;665(3):442–453. doi: 10.1016/0005-2760(81)90257-5. [DOI] [PubMed] [Google Scholar]
  24. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. White R. T., Damm D., Miller J., Spratt K., Schilling J., Hawgood S., Benson B., Cordell B. Isolation and characterization of the human pulmonary surfactant apoprotein gene. 1985 Sep 26-Oct 2Nature. 317(6035):361–363. doi: 10.1038/317361a0. [DOI] [PubMed] [Google Scholar]
  26. Whitsett J. A., Hull W., Ross G., Weaver T. Characteristics of human surfactant-associated glycoproteins A. Pediatr Res. 1985 May;19(5):501–508. doi: 10.1203/00006450-198505000-00018. [DOI] [PubMed] [Google Scholar]
  27. Williams M. C., Benson B. J. Immunocytochemical localization and identification of the major surfactant protein in adult rat lung. J Histochem Cytochem. 1981 Feb;29(2):291–305. doi: 10.1177/29.2.7019304. [DOI] [PubMed] [Google Scholar]

Articles from American Journal of Human Genetics are provided here courtesy of American Society of Human Genetics

RESOURCES