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. 1997 Apr;63(4):1594–1597. doi: 10.1128/aem.63.4.1594-1597.1997

Detection of Methylobacterium species by 16S rRNA gene-targeted PCR.

T Nishio 1, T Yoshikura 1, H Itoh 1
PMCID: PMC168451  PMID: 9097454

Abstract

We designed PCR primers for specific amplification of the 16S rRNA genes of seven species of the genus Methylobacterium. All of the pairwise species tested were successfully differentiated by PCR detection with a combination of five primer sets, with the exception of M. extorquens and M. rhodesianum. These primers did not cross-react with closely related bacteria in the alpha subclass tested.

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Selected References

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  1. Barany F. The ligase chain reaction in a PCR world. PCR Methods Appl. 1991 Aug;1(1):5–16. doi: 10.1101/gr.1.1.5. [DOI] [PubMed] [Google Scholar]
  2. Bratina B. J., Brusseau G. A., Hanson R. S. Use of 16S rRNA analysis to investigate phylogeny of methylotrophic bacteria. Int J Syst Bacteriol. 1992 Oct;42(4):645–648. doi: 10.1099/00207713-42-4-645. [DOI] [PubMed] [Google Scholar]
  3. Breslauer K. J., Frank R., Blöcker H., Marky L. A. Predicting DNA duplex stability from the base sequence. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3746–3750. doi: 10.1073/pnas.83.11.3746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801–4805. doi: 10.1073/pnas.75.10.4801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brusseau G. A., Bulygina E. S., Hanson R. S. Phylogenetic analysis and development of probes for differentiating methylotrophic bacteria. Appl Environ Microbiol. 1994 Feb;60(2):626–636. doi: 10.1128/aem.60.2.626-636.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Freier S. M., Kierzek R., Jaeger J. A., Sugimoto N., Caruthers M. H., Neilson T., Turner D. H. Improved free-energy parameters for predictions of RNA duplex stability. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9373–9377. doi: 10.1073/pnas.83.24.9373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Furuhata K., Koike K. A. [Isolation of Methylobacterium spp. from drinking tank-water and resistance of isolates to chlorine]. Nihon Koshu Eisei Zasshi. 1993 Nov;40(11):1047–1053. [PubMed] [Google Scholar]
  8. Gilardi G. L., Faur Y. C. Pseudomonas mesophilica and an unnamed taxon, clinical isolates of pink-pigmented oxidative bacteria. J Clin Microbiol. 1984 Oct;20(4):626–629. doi: 10.1128/jcm.20.4.626-629.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gilchrist M. J., Kraft J. A., Hammond J. G., Connelly B. L., Myers M. G. Detection of Pseudomonas mesophilica as a source of nosocomial infections in a bone marrow transplant unit. J Clin Microbiol. 1986 Jun;23(6):1052–1055. doi: 10.1128/jcm.23.6.1052-1055.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hanson R. S., Hanson T. E. Methanotrophic bacteria. Microbiol Rev. 1996 Jun;60(2):439–471. doi: 10.1128/mr.60.2.439-471.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Higgins D. G., Bleasby A. J., Fuchs R. CLUSTAL V: improved software for multiple sequence alignment. Comput Appl Biosci. 1992 Apr;8(2):189–191. doi: 10.1093/bioinformatics/8.2.189. [DOI] [PubMed] [Google Scholar]
  12. Hiraishi A., Furuhata K., Matsumoto A., Koike K. A., Fukuyama M., Tabuchi K. Phenotypic and genetic diversity of chlorine-resistant Methylobacterium strains isolated from various environments. Appl Environ Microbiol. 1995 Jun;61(6):2099–2107. doi: 10.1128/aem.61.6.2099-2107.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Neefs J. M., Van de Peer Y., Hendriks L., De Wachter R. Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Res. 1990 Apr 25;18 (Suppl):2237–2317. doi: 10.1093/nar/18.suppl.2237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Olsen G. J., Lane D. J., Giovannoni S. J., Pace N. R., Stahl D. A. Microbial ecology and evolution: a ribosomal RNA approach. Annu Rev Microbiol. 1986;40:337–365. doi: 10.1146/annurev.mi.40.100186.002005. [DOI] [PubMed] [Google Scholar]
  15. Ridgway H. F., Olson B. H. Chlorine resistance patterns of bacteria from two drinking water distribution systems. Appl Environ Microbiol. 1982 Oct;44(4):972–987. doi: 10.1128/aem.44.4.972-987.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tsuji K., Tsien H. C., Hanson R. S., DePalma S. R., Scholtz R., LaRoche S. 16S ribosomal RNA sequence analysis for determination of phylogenetic relationship among methylotrophs. J Gen Microbiol. 1990 Jan;136(1):1–10. doi: 10.1099/00221287-136-1-1. [DOI] [PubMed] [Google Scholar]
  17. Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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