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American Journal of Human Genetics logoLink to American Journal of Human Genetics
. 1986 May;38(5):641–648.

Chromosomal assignment of the genes for human aldehyde dehydrogenase-1 and aldehyde dehydrogenase-2.

L C Hsu, A Yoshida, T Mohandas
PMCID: PMC1684817  PMID: 3013004

Abstract

Chromosomal assignment of the genes for two major human aldehyde dehydrogenase isozymes, that is, cytosolic aldehyde dehydrogenase-1 (ALDH1) and mitochondrial aldehyde dehydrogenase-2 (ALDH2) were determined. Genomic DNA, isolated from a panel of mouse-human and Chinese hamster-human hybrid cell lines, was digested by restriction endonucleases and subjected to Southern blot hybridization using cDNA probes for ALDH1 and for ALDH2. Based on the distribution pattern of ALDH1 and ALDH2 in cell hybrids, ALDH1 was assigned to the long arm of human chromosome 9 and ALDH2 to chromosome 12.

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Selected References

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  1. Crow K. E., Kitson T. M., MacGibbon A. K., Batt R. D. Intracellular localisation and properties of aldehyde dehydrogenases from sheep liver. Biochim Biophys Acta. 1974 May 20;350(1):121–128. doi: 10.1016/0005-2744(74)90209-5. [DOI] [PubMed] [Google Scholar]
  2. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  3. Eckfeldt J. H., Yonetani T. Subcellular localization of the F1 and F2 isozymes of horse liver aldehyde dehydrogenase. Arch Biochem Biophys. 1976 Aug;175(2):717–722. doi: 10.1016/0003-9861(76)90564-6. [DOI] [PubMed] [Google Scholar]
  4. Goedde H. W., Harada S., Agarwal D. P. Racial differences in alcohol sensitivity: a new hypothesis. Hum Genet. 1979 Oct 2;51(3):331–334. doi: 10.1007/BF00283404. [DOI] [PubMed] [Google Scholar]
  5. Greenfield N. J., Pietruszko R. Two aldehyde dehydrogenases from human liver. Isolation via affinity chromatography and characterization of the isozymes. Biochim Biophys Acta. 1977 Jul 8;483(1):35–45. doi: 10.1016/0005-2744(77)90005-5. [DOI] [PubMed] [Google Scholar]
  6. Holmes R. S., Timms G. P. Mouse aldehyde dehydrogenase genetics: positioning of Ahd-1 on chromosome 4. Anim Blood Groups Biochem Genet. 1981;12(1):1–5. doi: 10.1111/j.1365-2052.1981.tb01524.x. [DOI] [PubMed] [Google Scholar]
  7. Hsu L. C., Tani K., Fujiyoshi T., Kurachi K., Yoshida A. Cloning of cDNAs for human aldehyde dehydrogenases 1 and 2. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3771–3775. doi: 10.1073/pnas.82.11.3771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ikawa M., Impraim C. C., Wang G., Yoshida A. Isolation and characterization of aldehyde dehydrogenase isozymes from usual and atypical human livers. J Biol Chem. 1983 May 25;258(10):6282–6287. [PubMed] [Google Scholar]
  9. Kitabatake N., Sasaki R., Chiba H. Localization of bovine liver aldehyde dehydrogenase isozymes and their immunological properties. J Biochem. 1981 Apr;89(4):1223–1229. [PubMed] [Google Scholar]
  10. Mohandas T., Sparkes R. S., Sparkes M. C., Shulkin J. D., Toomey K. E., Funderburk S. J. Regional localization of human gene loci on chromosome 9: studies of somatic cell hybrids containing human translocations. Am J Hum Genet. 1979 Sep;31(5):586–600. [PMC free article] [PubMed] [Google Scholar]
  11. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  12. Santisteban I., Povey S., West L. F., Parrington J. M., Hopkinson D. A. Chromosome assignment, biochemical and immunological studies on a human aldehyde dehydrogenase, ALDH3. Ann Hum Genet. 1985 May;49(Pt 2):87–100. doi: 10.1111/j.1469-1809.1985.tb01680.x. [DOI] [PubMed] [Google Scholar]
  13. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  14. Teng Y. S. Human liver aldehyde dehydrogenase in Chinese and Asiatic Indians: gene deletion and its possible implications in alcohol metabolism. Biochem Genet. 1981 Feb;19(1-2):107–114. doi: 10.1007/BF00486141. [DOI] [PubMed] [Google Scholar]
  15. Timms G. P., Holmes R. S. Genetics of aldehyde dehydrogenase isozymes in the mouse: evidence for multiple loci and localization of Ahd-2 on chromosome 19. Genetics. 1981 Feb;97(2):327–336. doi: 10.1093/genetics/97.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yen P. H., Marsh B., Mohandas T. K., Shapiro L. J. Isolation of genomic clones homologous to transcribed sequences from human X chromosome. Somat Cell Mol Genet. 1984 Nov;10(6):561–571. doi: 10.1007/BF01535221. [DOI] [PubMed] [Google Scholar]
  17. Yoshida A., Wang G., Davé V. Determination of genotypes of human aldehyde dehydrogenase ALDH2 locus. Am J Hum Genet. 1983 Nov;35(6):1107–1116. [PMC free article] [PubMed] [Google Scholar]
  18. Zaret K. S., Yamamoto K. R. Reversible and persistent changes in chromatin structure accompany activation of a glucocorticoid-dependent enhancer element. Cell. 1984 Aug;38(1):29–38. doi: 10.1016/0092-8674(84)90523-3. [DOI] [PubMed] [Google Scholar]
  19. von Bahr-Lindström H., Hempel J., Jörnvall H. The cytoplasmic isoenzyme of horse liver aldehyde dehydrogenase. Relationship to the corresponding human isoenzyme. Eur J Biochem. 1984 May 15;141(1):37–42. doi: 10.1111/j.1432-1033.1984.tb08152.x. [DOI] [PubMed] [Google Scholar]

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