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. 1997 May;63(5):2082–2085. doi: 10.1128/aem.63.5.2082-2085.1997

Identification of Salmonella abortusovis by PCR amplification of a serovar-specific IS200 element.

C R Beuzón 1, A Schiaffino 1, G Leori 1, P Cappuccinelli 1, S Rubino 1, J Casadesús 1
PMCID: PMC168497  PMID: 9143137

Abstract

Field and collection isolates of Salmonella abortusovis carry one IS200 element in a distinct chromosome location. IS200 is not found in the corresponding region of the chromosome of other Salmonella serovars. Sequencing of the boundaries of the S. abortusovis-specific IS200 insertion permitted the design of primers for the amplification of this IS200 element by PCR. Isolates of S. abortusovis are identified by the amplification of a DNA fragment of about 900 bp or larger. PCR amplification of DNA from salmonellae other than S. abortusovis yields either a fragment of about 200 bp or no product. The high specificity of the assay is confirmed by the absence of cross-reactivity with the following templates: (i) sheep DNA, (ii) DNAs from abortion-causing agents other than S. abortusovis, and (iii) DNAs from microorganisms that do not cause abortion but are common in flocks.

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Selected References

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