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. 1976 Sep;28(5):474–481.

A new pyruvate kinase variant (PK Osaka) demonstrated by partial purification and condensation.

K Shinohara, S Miwa, K Nakashima, E Oda, T Kageoka
PMCID: PMC1685104  PMID: 984043

Abstract

A qualitative variant of erythrocyte and liver pyruvate kinases (PK Osaka) was detected in a family in which two siblings have extremely low PK activity by semipurification with DEAE cellulose chromatography and subsequent concentration of the enzyme solutions. This was previously reported to be a quantitative variant based on studies of crude tissue preparations. The molecular aberrations were characterized by slow mobility upon electrophoresis, abnormal kinetics for phosphoenolpyruvate, and low affinity for anti-human erythrocyte PK serum. The mutant PK L was similar both electrophoretically and immunologically to PK R.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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