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. 1997 Jun;63(6):2449–2453. doi: 10.1128/aem.63.6.2449-2453.1997

Catalytic properties of the cellulose-binding endoglucanase F from Fibrobacter succinogenes S85.

S R Malburg 1, L M Malburg Jr 1, T Liu 1, A H Iyo 1, C W Forsberg 1
PMCID: PMC168539  PMID: 9172367

Abstract

The celF gene from the predominant cellulolytic ruminal bacterium Fibrobacter succinogenes encodes a 118.3-kDa cellulose-binding endoglucanase, endoglucanase F (EGF). This enzyme possesses an N-terminal cellulose-binding domain and a C-terminal catalytic domain. The purified catalytic domain displayed an activity profile typical of an endoglucanase, with high catalytic activity on carboxymethyl cellulose and barley beta-glucan. Immunoblotting of EGF and the formerly characterized endoglucanase 2 (EG2) from F. succinogenes with antibodies prepared against each of the enzymes demonstrated that EGF and EG2 contain cross-reactive epitopes. This data in conjunction with evidence that the proteins are the same size, share a 19-residue internal amino acid sequence, possess similar catalytic properties, and both bind to cellulose allows the conclusion that celF codes for EG2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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