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. 1997 Jul;63(7):2765–2770. doi: 10.1128/aem.63.7.2765-2770.1997

Benzoate degradation via the ortho pathway in Alcaligenes eutrophus is perturbed by succinate.

F Ampe 1, J L Uribelarrea 1, G M Aragao 1, N D Lindley 1
PMCID: PMC168572  PMID: 9212423

Abstract

During batch growth of Alcaligenes eutrophus on benzoate-plus-succinate mixtures, substrates were simultaneously metabolized, leading to a higher specific growth rate (mu = 0.56 h-1) than when a single substrate was used (mu = 0.51 h-1 for benzoate alone and 0.44 h-1 for succinate alone), without adversely affecting the growth yield (0.57 Cmol/Cmol). Flux distribution analysis revealed that succinate dehydrogenase most probably controls the rate of total succinate consumption (the maximum flux being 9.7 mmol.g-1.h-1). It is postulated that the relative consumption rate of each substrate is in part related to modified levels of gene expression but to a large extent is dependent upon the presence of succinate, end product of the beta-ketoadipate pathway. Indeed, the in vitro beta-ketoadipate-succinyl coenzyme A transferase activity was seen to be inhibited by succinate, a coproduct of the reaction.

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Selected References

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