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. 1997 Jul;63(7):2944–2948. doi: 10.1128/aem.63.7.2944-2948.1997

PCR-mediated detection of acidophilic, bioleaching-associated bacteria.

P De Wulf-Durand 1, L J Bryant 1, L I Sly 1
PMCID: PMC168590  PMID: 9212441

Abstract

The detection of acidophilic microorganisms from mining environments by culture methods is time consuming and unreliable. Several PCR approaches were developed to amplify small-subunit rRNA sequences from the DNA of six bacterial phylotypes associated with acidic mining environments, permitting the detection of the target DNA at concentrations as low as 10 fg.

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Selected References

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