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. 1997 Aug;63(8):3286–3290. doi: 10.1128/aem.63.8.3286-3290.1997

Glutathione S-transferase-encoding gene as a potential probe for environmental bacterial isolates capable of degrading polycyclic aromatic hydrocarbons.

G Lloyd-Jones 1, P C Lau 1
PMCID: PMC168628  PMID: 9251217

Abstract

Homologs of the glutathione S-transferase (GST)-encoding gene were identified in a collection of aromatic hydrocarbon-degrading Sphingomonas spp. isolated from New Zealand, Antarctica, and the United States by using PCR primers designed from the GST-encoding gene of Sphingomonas paucimobilis EPA505. Sequence analysis of PCR fragments generated from these isolates and of the GST gene amplified from DNA extracted from polycyclic aromatic hydrocarbon (PAH)-contaminated soil revealed a high degree of conservation, which may make the GST-encoding gene a potentially useful marker for PAH-degrading bacteria.

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Selected References

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  1. Armstrong R. N. Glutathione S-transferases: structure and mechanism of an archetypical detoxication enzyme. Adv Enzymol Relat Areas Mol Biol. 1994;69:1–44. doi: 10.1002/9780470123157.ch1. [DOI] [PubMed] [Google Scholar]
  2. Balkwill D. L., Drake G. R., Reeves R. H., Fredrickson J. K., White D. C., Ringelberg D. B., Chandler D. P., Romine M. F., Kennedy D. W., Spadoni C. M. Taxonomic study of aromatic-degrading bacteria from deep-terrestrial-subsurface sediments and description of Sphingomonas aromaticivorans sp. nov., Sphingomonas subterranea sp. nov., and Sphingomonas stygia sp. nov. Int J Syst Bacteriol. 1997 Jan;47(1):191–201. doi: 10.1099/00207713-47-1-191. [DOI] [PubMed] [Google Scholar]
  3. Berthelet M., Whyte L. G., Greer C. W. Rapid, direct extraction of DNA from soils for PCR analysis using polyvinylpolypyrrolidone spin columns. FEMS Microbiol Lett. 1996 Apr 15;138(1):17–22. doi: 10.1111/j.1574-6968.1996.tb08128.x. [DOI] [PubMed] [Google Scholar]
  4. Fredrickson J. K., Balkwill D. L., Drake G. R., Romine M. F., Ringelberg D. B., White D. C. Aromatic-degrading Sphingomonas isolates from the deep subsurface. Appl Environ Microbiol. 1995 May;61(5):1917–1922. doi: 10.1128/aem.61.5.1917-1922.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Furukawa K., Hayase N., Taira K., Tomizuka N. Molecular relationship of chromosomal genes encoding biphenyl/polychlorinated biphenyl catabolism: some soil bacteria possess a highly conserved bph operon. J Bacteriol. 1989 Oct;171(10):5467–5472. doi: 10.1128/jb.171.10.5467-5472.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Furukawa K., Simon J. R., Chakrabarty A. M. Common induction and regulation of biphenyl, xylene/toluene, and salicylate catabolism in Pseudomonas paucimobilis. J Bacteriol. 1983 Jun;154(3):1356–1362. doi: 10.1128/jb.154.3.1356-1362.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gibson D. T., Mahadevan V., Jerina D. M., Yogi H., Yeh H. J. Oxidation of the carcinogens benzo [a] pyrene and benzo [a] anthracene to dihydrodiols by a bacterium. Science. 1975 Jul 25;189(4199):295–297. doi: 10.1126/science.1145203. [DOI] [PubMed] [Google Scholar]
  8. Gish W., States D. J. Identification of protein coding regions by database similarity search. Nat Genet. 1993 Mar;3(3):266–272. doi: 10.1038/ng0393-266. [DOI] [PubMed] [Google Scholar]
  9. Hayes J. D., Pulford D. J. The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol. 1995;30(6):445–600. doi: 10.3109/10409239509083491. [DOI] [PubMed] [Google Scholar]
  10. Hofer B., Backhaus S., Timmis K. N. The biphenyl/polychlorinated biphenyl-degradation locus (bph) of Pseudomonas sp. LB400 encodes four additional metabolic enzymes. Gene. 1994 Jun 24;144(1):9–16. doi: 10.1016/0378-1119(94)90196-1. [DOI] [PubMed] [Google Scholar]
  11. Kim E., Aversano P. J., Romine M. F., Schneider R. P., Zylstra G. J. Homology between genes for aromatic hydrocarbon degradation in surface and deep-subsurface Sphingomonas strains. Appl Environ Microbiol. 1996 Apr;62(4):1467–1470. doi: 10.1128/aem.62.4.1467-1470.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. La Roche S. D., Leisinger T. Sequence analysis and expression of the bacterial dichloromethane dehalogenase structural gene, a member of the glutathione S-transferase supergene family. J Bacteriol. 1990 Jan;172(1):164–171. doi: 10.1128/jb.172.1.164-171.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lau P. C., Garnon J., Labbé D., Wang Y. Location and sequence analysis of a 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-encoding gene (bpdF) of the biphenyl/polychlorinated biphenyl degradation pathway in Rhodococcus sp. M5. Gene. 1996 May 24;171(1):53–57. doi: 10.1016/0378-1119(96)00025-x. [DOI] [PubMed] [Google Scholar]
  14. McCarthy D. L., Navarrete S., Willett W. S., Babbitt P. C., Copley S. D. Exploration of the relationship between tetrachlorohydroquinone dehalogenase and the glutathione S-transferase superfamily. Biochemistry. 1996 Nov 19;35(46):14634–14642. doi: 10.1021/bi961730f. [DOI] [PubMed] [Google Scholar]
  15. Mueller J. G., Chapman P. J., Blattmann B. O., Pritchard P. H. Isolation and characterization of a fluoranthene-utilizing strain of Pseudomonas paucimobilis. Appl Environ Microbiol. 1990 Apr;56(4):1079–1086. doi: 10.1128/aem.56.4.1079-1086.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nishida M., Kong K. H., Inoue H., Takahashi K. Molecular cloning and site-directed mutagenesis of glutathione S-transferase from Escherichia coli. The conserved tyrosyl residue near the N terminus is not essential for catalysis. J Biol Chem. 1994 Dec 23;269(51):32536–32541. [PubMed] [Google Scholar]
  17. Orser C. S., Dutton J., Lange C., Jablonski P., Xun L., Hargis M. Characterization of a Flavobacterium glutathione S-transferase gene involved reductive dechlorination. J Bacteriol. 1993 May;175(9):2640–2644. doi: 10.1128/jb.175.9.2640-2644.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Perito B., Allocati N., Casalone E., Masulli M., Dragani B., Polsinelli M., Aceto A., Di Ilio C. Molecular cloning and overexpression of a glutathione transferase gene from Proteus mirabilis. Biochem J. 1996 Aug 15;318(Pt 1):157–162. doi: 10.1042/bj3180157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ponce M. R., Micol J. L. PCR amplification of long DNA fragments. Nucleic Acids Res. 1992 Feb 11;20(3):623–623. doi: 10.1093/nar/20.3.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Suyama A., Iwakiri R., Kimura N., Nishi A., Nakamura K., Furukawa K. Engineering hybrid pseudomonads capable of utilizing a wide range of aromatic hydrocarbons and of efficient degradation of trichloroethylene. J Bacteriol. 1996 Jul;178(14):4039–4046. doi: 10.1128/jb.178.14.4039-4046.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994 Nov 11;22(22):4673–4680. doi: 10.1093/nar/22.22.4673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Vuilleumier S. Bacterial glutathione S-transferases: what are they good for? J Bacteriol. 1997 Mar;179(5):1431–1441. doi: 10.1128/jb.179.5.1431-1441.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wang Y., Lau P. C., Button D. K. A marine oligobacterium harboring genes known to be part of aromatic hydrocarbon degradation pathways of soil pseudomonads. Appl Environ Microbiol. 1996 Jun;62(6):2169–2173. doi: 10.1128/aem.62.6.2169-2173.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wang Y., Lau P. C. Sequence and expression of an isocitrate dehydrogenase-encoding gene from a polycyclic aromatic hydrocarbon oxidizer, Sphingomonas yanoikuyae B1. Gene. 1996 Feb 2;168(1):15–21. doi: 10.1016/0378-1119(95)00732-6. [DOI] [PubMed] [Google Scholar]
  25. White D. C., Sutton S. D., Ringelberg D. B. The genus Sphingomonas: physiology and ecology. Curr Opin Biotechnol. 1996 Jun;7(3):301–306. doi: 10.1016/s0958-1669(96)80034-6. [DOI] [PubMed] [Google Scholar]
  26. Williams P. A., Murray K. Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid. J Bacteriol. 1974 Oct;120(1):416–423. doi: 10.1128/jb.120.1.416-423.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Zablotowicz R. M., Hoagland R. E., Locke M. A., Hickey W. J. Glutathione-s-transferase activity and metabolism of glutathione conjugates by rhizosphere bacteria. Appl Environ Microbiol. 1995 Mar;61(3):1054–1060. doi: 10.1128/aem.61.3.1054-1060.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Zylstra G. J., Gibson D. T. Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli. J Biol Chem. 1989 Sep 5;264(25):14940–14946. [PubMed] [Google Scholar]

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